Objective To investigate the effect of different analgesia during labor and their side effect to the neonates. Methods Totally 130 puerperas were randomly divided into three groups: group A included 50 puerperas with analgesia by GT-4A workstation; group B included of 40 puerperas with mixed gas analgesia consisted of 50% of nitric oxide and 50% of oxygen; and group C(40 gravidas) as control group. The analgesic effect, duration of labor, mode of delivery and the amount of postpartum hemorrhage were observed. Results The analgesic effect in group A was significantly better than group B ( P

0.05). Conclusion Spasfon can effectively improve cervical dilatation during labor and it is well tolerated by both mother and newborn.

Objective To investigate the effect of cluster nursing on quality of life and mental state of cervical cancer patients receiving chemotherapy. Methods 72 cervical cancer patients receiving chemotherapy were randomly divided into control group and observation group.The control group was given routine care and the observation group was given cluster nursing on the basis of routine care.The quality of life core questionnaire (EORTC QOL-C30) and Symptom Checklist SCL-90 score were tested at the end of treatment. Results Compared with the control group, after using cluster nursing, the EORTC QOL-C30 functional areas of physical function role function, cognitive function , social function, emotional function of patients in the observation group were significantly improved( <0.05), the main symptoms of nausea areas vomiting and pain were decreased significantly ( <0.05),and sleep,diet,diarrhea and constipation were improved significantly (<0.05) . SCL-90 scale showed that the somatization,obsessive-compulsive symptoms,depression and anxiety in observation group were decreased significantly compared with the control group ( <0.05),the somatization, obsessive-compulsive symptoms and anxiety were increased significantly in control group compared with China norm (<0.05) .Conclusion Cluster nursing on patients with cervical cancer receiving chemotherapy can effectively improve quality of life and reduce the incidence of negative emotions.

Objective : To investigate the effect of exposure to low concentrations of benzene on miR-155 and miR-223 expression in peripheral blood lymphocytes among workers with benzene exposure. Methods : A hundred male employees at a risk of exposure to benzene (the exposed group) were randomly sampled from two small metal products manufacturing enterprises and one medium-sized chemical raw material and chemical products manufacturing enterprise in Ningbo City, Zhejiang Province, and 60 age-matched male employees without benzene exposure were randomly selected as the unexposed group. Age, body mass index ( BMI ), smoking status, alcohol consumption, disease history, medication history and routine blood testing results of subjects were collected using a questionnaire survey. The 8-hour time weighted average concentration ( CTWA ) of benzene was measured in the workplace using thermal desorption gas chromatography, and the urine 8-hydroxy-2' deoxyguanosine ( 8-OHdG ) levels were determined using high-performance liquid-chromatography tandem mass spectrometry (HPLC-MS/MS). The miR-155 and miR-223 expression was quantified in peripheral blood lymphocytes using quantitative fluorescent reverse transcription-polymerase chain reaction assay, and the factors affecting miR-155 and miR-223 expression were identified using multivariable logistic regression analysis. Results : The subjects in the exposed group had a mean age of ( 31.17±7.30 ) years, and were exposed to low concentrations of benzene ( CTWA, 0.05 to 0.30 mg/m3 ) , while the subjects in the unexposed group had a mean age of ( 32.52±6.15 ) years. There were no significant differences between the exposed and unexposed groups in terms of age, BMI, proportion of smokers or proportion of alcohol consumers ( P>0.05 ). There was no significant difference in the median relative miR-155 expression between the exposed and unexposed groups ( 0.953 vs. 1.293, P>0.05 ), and lower median relative miR-223 expression was quantified in the exposed group than in the unexposed group ( 0.540 vs. 1.433, P<0.05 ). Multivariable logistic regression analysis revealed that down-regulation of miR-223 expression correlated with exposure to benzene ( OR=2.719, 95%CI: 1.308-5.651 ). Conclusion Down-regulation of miR-223 expression may be associated with exposure to low concentrations of benzene.

Objective To explore the application of endometriosis fertility index (EFI) in guidance after laparoscopic surgery of endometriosis patients combined with infertility and to explore methods to improve pregnancy rate in different EFI groups. Methods A prospective research was done in endometriosis patients combined with infertility in Beijing Obstetrics and Gynecology Hospital from January 2010 to June 2011, after laparoscopic surgery, these 146 patients were divided into 3 groups by EFI score. Using different pregnancy guidance, these patients had 5 years follow-up. Results (1) The 5 years overall pregnancy rate was 89.0%(130/146). The pregnancy rate was 95.7%(45/47) in EFI≥9 group, 92.8%(77/83) in EFI 5-8 group and 8/16 in EFI≤4 group, three groups were all reach satisfactory pregnancy rate;the rate of the first two groups had no statistically significance (P=0.498), but had significant difference with the last group (P<0.01). (2) In EFI≥5 patients, pregnancy rate was the highest in 6 months after operation;in EFI≥9 group, the pregnancy rate was 66.7%(30/45), and EFI 5-8 group was 50.6%(39/77). (3) EFI≥9 group had the highest natural pregnancy rate [83.6% (46/55)], natural pregnancy rate was significant statistical different in different EFI groups (P=0.001). Conclusions EFI score is a useful evaluation in predicting and guiding pregnancy in endometriosis patients combined with infertility after laparoscopic surgery. EFI score guidance, strict post-operation management and positive pregnancy scheme could significantly improve the pregnancy rate of endometriosis patients with infertility.