Objective: To investigate the clinical features of chronic renal failure (CRF) complicated by primary hypothyroidism.Methods: Thyroid function, thyroid microsome antibody ( TM), and thyroid globulin antibody (TG) were determined withradioimmunoassay in 23 CRF complicated by primary hypothyroidism patients, and the serum calcium, phosphorus, potassi-um, cholesterol, glucose, urea nitrogen, hemoglobulin, and endogenous creatinine clearance rate were also assayed. Thirty-onepatients with only CRF were in control group. Results:The level of thyroid-stimulating hormone (TSH), Ch, and Ca in pa-tients with CRF complicated by primary hypothyroidism were higher than that in control group,and serum free T4 (FT4),K,and P were lower than that in control group. The others had no difference between two groups. TG and TM increased in2 cases. Conclusion:The most important criteria in diagnosing CRF complicated by primary hypothyroidism is TSH twotimes higher than normal upper limit. The others include decreased FT4, normal level of Ca, P, and K, and increased Ch,TG,and TM. The diagnosis should be performed combined with clinical manifestations.

Objective:To study the medicine storage management mode in our hospital. Methods: The medicine sales and inventory data were obtained from 2010 to 2013 by the HIS of our hospital. Medicine storage turnover ratio was calculated by using Microsoft Excel. The efficiency in the inventory management was evaluated by medicine storage turnover ratio. The storage control in our hospital was analyzed by the relevant references. Results: The medicine storage turnover ratio from 2010 to 2013 in our hospital was improved to 20. 06 times, the turnover days was reduced to 18 days which met the criteria for the review of the grade A class 3 hospital. Conclusion: The medicine storage management should be scientifically adjusted on the basis of the clinical characteristics and the use of medicines in the hospital. In this way, the improvement of medicine storage turnover ratio, the optimized control of the medicine stocks and the improvement of the social and economic benefit of the hospital will be realized.

Objective:To evaluate the performance of reticulocyte (Ret) detected by Sysmex XN-3000 automatic blood analyzer.Methods: 96 blood samples were collected randomly in clinical detection of reticulocytes, and respectively were detected by XN-3000 automatic blood analyzer and microscopy. The precision, stability, linearity and carryover of Ret in blood samples detected by XN-3000 automatic blood analyzer were evaluated by the correlation analysis of the results from the two methods and the correlation analysis would be completed by microscopy.Results: precision, linear range and sensitivity of Ret measured by XN-3000 were within the allowable range. The stability did not change significantly below 4℃ within 48h (CV<1%). The carryover rate was 0.14%. The results measured by XN-3000 were well compared with microscopy (r=0.979).Conclusion: Sysmex XN-3000 automatic blood analyzer is applied to the clinical detection of large quantities of specimens and it is ideal for measuring Ret.

Objective To investigate the influence of renal function on serum BNP in the diagnosis of CRF with heart failure by observing the relationship between eGFR and BNP in serum and comparing cut-off values of BNP in different eGFR levels. Methods The elderly participants were enrolled in the study, including 52 patients with heart failure, and 29 patients without heart failure and 30 healthy controls. Serum BNP level was measured by ELISA.Results The level of serum BNP increased significantly in subjects with heart failure compared with those with renal dysfunction for no-heart failure patients (P ＜ 0.05) and healthy controls. BNP level was significantly higher in CRF no-heart failure patients than in control subjects. eGFR showed negative correlation with BNP in ESRD no-heart failure patients (γ= -0. 581, P ＜ 0.01). There was no correlation between eGFR and ESRD with heart failure patients (γ= - 0.081, P ＞ 0.05). The AUC of BNP in patients (eGFR 30 ～ 60 ml) was 0. 951, when cut-off value was 1 500 ng/L,the sensitivity and specificity of BNP were 96.4% and 86. 7% respectively. The AUC of BNP in patients(eGFR ＜30 ml)was 0. 860, when cut-off value was 1 850 ng/L,the sensitivity and specificity of B NP were 66.7% and 92.9%respectively. Conclusions Heart failure was major factor result in higher levels of BNP in chronic renal failure with heart failure patients. BNP could be used as a diagnostic marker for CRF with heart failure patients.

Objective To investigate principle and clinic pathologic characteristics of autoimmune thyroid disease(AITD) associated nephrosis. Methods By introducing 5 cases and related reference to review clinic pathologic characteristics of AITD associated nephrosis. Results AITD associated nephrosis regard proteinuria as principal, and a handful of performance is nephritic syndrome and nephritis syndrome. Pathologic characteristics are diversity. It is usually membranous nephrology, mesangial proliferative glomerulonephritis and focal and sclerosing glomerulonephritis. Conclusion Treatment of AITD associated nephrosis itself and immunosuppressive agent therapy might be useful to get remission of it.

A rapid, sensitive, label-free and separation-free analytical method for determination of melamine ( MA) was developed based on surface enhanced Raman scattering ( SERS ) effect of gold nanoparticles. Through tri-sodium citrate reduction method, gold nanoparticles with average diameter of 30 nm were obtained. The melamine detection platform was constructed after self-assembling 4-mercapto phenylboronic acid (4-MPBA) on the surface of gold nanoparticles through Au S covalent bond. When MA existed in solution, 4-MPBA functionalized gold nanoparticles would aggregate because of strong hydrogen bond interaction between MA and 4-MPBA. Moreover, following increase of the concentration of MA, gold nanoparticles would aggregate more intensively and form more hot spots. As a result, Raman signal of 4-MPBA and MA was enhanced greatly. The characteristic Raman peaks of 4-MPBA and MA respectively located at 1076 cm-1 and 715 cm-1 . Hence, the qualitative and quantitative detection for MA were realized based on the ratio value of I715 cm-1 to I1076 cm-1 . The linear range of MA detection was 0 . 1 μmol/L-1. 5 μmol/L. The limit of detection (LOD) reached 0. 02 μmol/L in terms of three times signal to noise.

Objective To investigate the training needs of health emergency professionals in centers for disease control and prevention (CDC) ,and to provide evidence for making training plan .Methods Totally 66 health emergency professionals who par‐ticipated in health emergency training class of CDC were surveyed with questionnaires in July ,2014 .The items included training content ,mode ,time ,teachers ,assessment forms and graduation way .Results Forty one persons (62 .12% of all subjects) selected health emergency disposal of all kinds of emergencies as the training contents ,and case analysis as the training mode .There were no statistical significances for the differences of the proportions of the professional staffs between different genders ,education levels ,ti‐tles ,categories and agencies (P>0 .05) .52 persons (78 .79% ) considered that training frequency of 1-2 times per year was appro‐priate ,and 53 persons (80 .30% ) considered that the most appropriate duration for each training was 2 -3 days .Domestic experts as a training teacher had the highest proportion (56 .06% ) ,followed by health emergency management cadres (34 .85% ) ,and foreign experts (6 .06% ) .The proportion of selecting university professor as a training teacher was lowest (3 .03% ) .71 .21% (47 persons) selected analog dealing with practical problems as assessment form ,and 71 .21% (47 persons) selected granting credits as graduation way .Gender and agencies were two important influencing factors for selecting different graduation ways (P< 0 .05) . Conclusion Training program of health emergency should be made according to the training needs .Appropriate training content and form should be selected in order to improve the quality and effectiveness of training ,and to improve the ability of the health e‐mergency professionals .

OBJECTIVETo study the chemical constituents from Hypericum perforatum.

METHODCompouds were isolated by chromatographic techniques. Their structures were identified by spectral methods. The inhibitory activity of recombinant human PTP1B was evaluated.

RESULTNine compounds were elucidated as D-Mannitol (1), 1,2-benzenedicarboxylic acid bis(1-methylpropyl) ester (2), (7E, 6R,9S)-9-hydroxy-4,7-megastigmadien-3-one (3), (6S,9R)-roseoside (4) , 2,6-dimethoxy-4-hydroquinone-l-O-beta-D-glucopyranoside (5), 2,6-di-methoxy-4-hydroxybenzyl alcohol 1 -O-beta-D-glucopyranoside (6), syringate 4-O-beta-glucopyranoside (7), hypericin (8), skyrin-6-0-beta-D-glucopyranoside (9) , (R)-2,3-dihydroxypropyl 3,4-dihydroxy-benzoate (10). At a concentration of 2 micromol x L(-1), compound 8 inhibited recombinant human PTP1B with inhibitory rate of 96.4% and IC50 of 2.5 micromol x L(-1).

CONCLUSIONcompound 10 was new, compounds 2-4, 7 were obtained from Hypericum for the first time and compounds 5-6 was isolated from this plant for the first time. Compound 8 showed remarkable PTP1B inhibitory activity.

Anthraquinones ; chemistry ; isolation & purification ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Hypericum ; chemistry ; Molecular Structure ; Norisoprenoids ; chemistry ; isolation & purification

The chemical constituents of Parthenocissus quinque were investigated. The chemical constituents were isolated by column chromatography on silical gel and sephadex LH-20. Their structures were elucidated on the basis of spectral analysis and of comparison of physical constant. Nine compounds were isolated from this plant and the structures of them were identified as 3,4,5-trihydroxy- benzoic acid (1), piceatannol (2), resveratrol (3), resveratrol trans-dehydrodimer (4), cyphoste mmin B (5), pallidol (6), cyphostemmin A (7), quercetin-3-O-alpha-L-rhamnoside (8), myricetin-3-O-alpha-L-rhamnoside (9), respectively. Compounds 1, 4-9 were isolated from this plant for the first time.
Plant Extracts ; analysis ; isolation & purification ; Vitaceae ; chemistry

Objective To explore the characteristics regarding temporal,spatial and spatiotemporal distribution on severe fever with thrombocytopenia syndrome (SFTS) in Henan province.Methods Surveillance data related to SFTS was collected in Henan province,from year 2014 to 2016.Descriptive method was used to analyze the distribution of SFTS.1.7.0 software related to the Public health geographic information system (PHGIS),was applied to draw the spatial distribution map of SFTS.Chi-square test was used to compare the different incidence rates.Results A total of 2 781 SFTS cases,including 34 deaths,were reported in Henan province from 2014 to 2016,with an average annual fatality rate as 1.22％.There were statistically significant differences for the incidence rates of SFTS between different years (P＜0.01).Cases were mainly concentrated from April to October,which accounted for 96.66％ of the total number,with the incidence peak seen in May.Incidence rates of SFTS in spring,summer,autumn were higher than that in winter.The cases were scattering around in 26 counties of 8 cities.Xinyang city reported 2 714 cases,accounting for 97.59％ of the total number of cases in the province.The average annual incidence rate in Xinyang city was 17.22 per 100 000,much higher than that for the whole Henan province (0.98 per 100 000),with statistically significant difference (P＜0.01).Six counties reported having death cases,that accounted for 23.08％ of the total number of counties,reported to have death cases.Two kinds of incidence patterns of SFTS were noticed in Henan province,with aggregation in some local regions or sporadic in individual counties.The number of counties with reporting cases increased annually.The epidemic area was expanding and gradually spreading from south to north areas of the province.Conclusions SFTS was characterized with both temporal and spatial clusters in Henan province.Effective prevention and control measures should be made in accordance with the spatiotemporal distribution and the trend on SFTS.