Objective To compare the performance of high‐throughput ELISA and ECLIA in the determination of carcinoembry‐onic antigen (CEA) .Methods The CEA concentration of serum samples were respectively determinate by high‐throughput ELISA and ECLIA ,and the results were compared .Results Two kinds of detection methods could both accurately reflect the concentration of serum CEA .There was no significant difference between the results of two methods (P> 0 .05) .Within the linear range ,the CEA result of high‐throughput was correlate closely with that of ECLIA (r=0 .922 8 ,P<0 .01) .Conclusion High‐throughput ELISA can accurately detect the serum CEA concentration .

Purpose To study the expression of TMPRSS2, ERG and ETV1 in prostatic cancer and their clinical pathologic signifi-cance. Methods Tissue microarray and immunohistochemistry (MaxVision) were used to detect TMPRSS2, ERG and ETV1 expres-sion in 70 prostatic cancer tissues, 10 prostatic intraepithelial neoplasia tissues and 18 benign prostate tissues. Results There was no statistical significance on positive rate of the expression of TMPRSS2 among prostatic cancer tissues, prostatic intraepithelial neoplasia tissues and benign prostate tissues (P>0. 05). The positive rate (81. 4%) of ERG in prostatic cancer tissues was significantly higher than that in prostatic intraepithelial neoplasia tissues ( 30. 0%) and benign prostate tissues ( 0. 0 ) ( P <0. 05 ) . The positive rate (68. 6%) of ETV1 in prostatic cancer tissues was significantly higher than that in prostatic intraepithelial neoplasia tissues (50. 0%) and benign prostate tissues (22. 2%) (P<0. 05). There was no correlation among the positive rates of TMPRSS2, ERG and ETV1 in prostatic cancer tissues and age (P>0. 05). The expression of TMPRSS2, ERG and ETV1 was positively correlated to Gleason score and clinical stage (P<0. 05). TMPRSS2 had positive correlation with ERG (rs =0. 465, P<0. 01). TMPRSS2 had positive correla-tion with ETV1 (rs =0. 590, P<0. 01). ERG had no positive correlation with ETV1 (rs =0. 151, P>0. 05). Conclusion ERG and ETV1 are expected to become therapeutic targets for prostate cancer. Detecting TMPRSS2, ERG and ETV1 at the same time is helpful to diagnosis and differential diagnosis of prostatic cancer, which might be new molecule markers of prostate cancer.

Objective:To establish and evaluate the clinical prediction model of stigma in patients with lymphoma so as to provide a basis for the assessment and response of stigma in patients with lymphoma.Methods:Convenience sampling method was used to select 130 patients with lymphoma who were hospitalized in Jiangsu Cancer Hospital from June to December 2019. We collected patients' clinical data, and assessed patients' quality of life and stigma. The R software was used to analyze and process the data, and screened characteristic factors were predicted by combining with the LASSO regression model. Multivariate Logistic regression analysis was used to analyze the influencing factors of stigma in patients with lymphoma. We constructed a nomogram model to evaluate the identification, calibration and clinical applicability of the predictive model through C index, calibration chart and decision curve analysis.Results:A total of 120 patients' valid data were gathered. Patients with lymphoma had a high level of stigma. The predictive factors of the predictive model included whether to be hospitalized for the first time, age, patient's medical insurance type, scores of the thirtieth and twenty-seventh question of the quality of life questionnaire. The model showed good predictive ability. The C index of training group, overall sample and validation group were 0.824, 0.776 and 0.684 respectively, and the C index performed well.Conclusions:The nomogram model developed in this research can help clinical nurses early identify lymphoma patients with a high level stigma, and give targeted response plans, which can greatly improve the quality of life and prognosis of patients, and is worthy of clinical application.

Objective To study the diagnostic value of hepatobiliary imaging at 6 h post-injection of 99 Tcm-diethyl iminodiacetic acid ( EHIDA) ( HI-6) combined with serum gamma-glutamyltransferase (γ-GT)/total bile acid ( TBA) ratio ( combined diagnosis) in children with congenital extrahepatic biliary atre-sia (CEBA). Methods Clinical data of 194 pediatric patients (118 males, 76 females, mean 70.9 d) with jaundice from May 2013 to March 2017 were retrospectively analyzed. All patients underwent surgery. According to the operation and pathologic diagnosis, patients were divided into CEBA group ( 113 cases) , infant hepatitis syndrome ( IHS) group ( 81 cases) . Serumγ-GT, TBA and the ratio ofγ-GT/TBA of the 2 groups were compared. Receiver operating characteristic ( ROC) curves were drawn to determine threshold values of the 3 parameters for diagnosis of CEBA. Patients also underwent 99 Tcm-EHIDA hepatobiliary dy-namic imaging. The diagnostic efficacies of HI-6, γ-GT, γ-GT/TBA ratio, and combined diagnosis were compared. Two-sample t test and χ2 test were used to analyze the data. Results There were significant differences in γ-GT/TBA ratio,γ-GT and TBA content between the 2 groups (t values:8.217, 9.298 and 2.426, all P<0.05). The serumγ-GT andγ-GT/TBA ratio had high diagnostic accuracies (area under ROC curve ( AUC):0.884 and 0.863) . The sensitivity, specificity, accuracy and positive predictive value of HI-6 in the diagnosis of CEBA were 91.15％(103/113), 39.51％(32/81), 69.59％(135/194) and 67.76％(103/152);the parameters of γ-GT were 69.91％(79/113), 93.83％(76/81), 79.90％(155/194) and 94.05％(79/84);and those ofγ-GT/TBA ratio were 71.68％(81/113), 92.59％(75/81), 80.41％(156/194) and 93.10％(81/87). The sensitivity of HI-6 was significantly higher than that of γ-GT and GGT/TBA ratio (χ2 values:16.256 and 14.154, both P<0.05) , but the specificity, accuracy and positive predic-tive value were significantly lower than those ofγ-GT/TBA ratio (χ2 values:50.899, 6.062 and 20.054, all P<0.05). The specificity, accuracy and positive predictive value of the combined diagnosis were 95.06％(77/81), 92.78％(180/194) and 96.26％(103/107) respectively, which were significantly higher than those of HI-6 (χ2 values:56.786, 34.168 and 31.335, all P<0.05) . Conclusions HI-6 combined withγ-GT/TBA ratio can significantly improve the diagnostic specificity and accuracy for CEBA. This method is more time-saving, simple and reliable, and has important clinical value.

Objective To explore the application effect of constrained patrol record form in safety management of agitated patients in neurosurgery. Methods A total of 84 cases of agitated patients with brain injury in the neurosurgery of our hospital from October 2014 to March 2015 were selected and divided into the control group and the observation group by random number table method, with 42 cases in each group. Patients in the control group were given routine constraint care management, while patients in the observation group were given safety management with the use of constrained patrol record form. The quality of constraint care was compared between two groups. Satisfaction of the nurses to the record form and the satisfaction of patients and their families to nursing were also compared between two groups. Results Before the application of constrained patrol record form, the number of complaints in the control group was 16, and the writing quality of nurses′constrained patrol record form was (91.42±10.21); after the application of constrained patrol record form, the number of complaints was 2 and the writing quality of record form was (97.12±14.11) (P<0.05). During the constrained period, the incidence rate and the number of cases of complications in the observation group were lower than that in the control group; and the nurses′s satisfaction to record form and patients′ satisfaction to nursing in the observation group were higher than that in the contro group ( P<0. 05 ) . Conclusions The application of constrained patrol record form in the safety management of agitated patients in neurosurgery, can effectively improve the nursing quality of agitated patients, reduce the occurrence of accidents, protect the safety of patients and improve the satisfaction of patients and their families.

Objective To explore the impacts of the personalized nursing interventions on medical compliance of patients with rheumatoid arthritis ( RA) . Methods A total of 60 patients with RA were randomly classified into experimental group ( 30 cases ) and control group ( 30 cases ) . The patients of control group received routine medicine treatment and specialist nursing care. In the experimental group, personal profiles were established and all patients of the experimental group applied personalized nursing interventions based on control group. The rehabilitation status and medical compliance were observed before and after interventions in two groups, and treatment efficacy was evaluated by American College of Rheumatology ( ACR) and compared and analyzed between two groups. Results After 12 weeks nursing interventions, the improvement of ACR20 in the experimental group was better than that of the control group (χ2 =4. 344, P <0. 05 ); early morning stiffness, joint swelling and tenderness of the experimental group ameliorated compared with the control group (t= -4. 524,-8. 283,-5. 842;P<0. 01); the difference of medical compliance was statistical significant between two groups (χ2 =10. 76,P < 0. 05). Conclusions For patients with RA, personalized nursing interventions can effectively improve their medical compliance and clinical efficacy.

Objective:To explore the correlation among resilience, self-management and fear of progression in patients with inflammatory bowel disease, so as to provide a reference for clinical treatment and nursing of patients.Methods:From January 2020 to December 2021, 141 patients with inflammatory bowel disease who were hospitalized in the Department of Gastroenterology, the Affiliated Huaian First People's Hospital of Nanjing Medical University, were selected by convenience sampling as the research object. Questionnaires were conducted on the patients using the General Information Questionnaire, the Self-Management Behavior Scale for Patients with Inflammatory Bowel Disease, Connor-Davidson resilience scale (CD-RISC) , and the Fear of Progression Questionnaire-Short Form (FoP-Q-SF) .Results:Among 141 patients with inflammatory bowel disease, the total scores of the Self-Management Behavior Scale for Patients with Inflammatory Bowel Disease, CD-RISC, and FoP-Q-SF were (126.69±26.67) , (67.65±12.52) , and (30.25±9.23) , respectively. Pearson correlation analysis showed that the total score of the CD-RISC in patients with inflammatory bowel disease was negatively correlated with the total score of the FoP-Q-SF ( r=-0.68, P<0.05) . The total score of the Self-Management Behavior Scale for Patients with Inflammatory Bowel Disease was negatively correlated with the total score of the FoP-Q-SF ( r=-0.52, P<0.05) , and the total score of the CD-RISC was positively correlated with the total score of the Self-Management Behavior Scale for Patients with Inflammatory Bowel Disease ( r=0.72, P<0.05) . Conclusions:Both resilience and self-management in patients with inflammatory bowel disease were negatively correlated with fear of progression. Medical and nursing staff can enhance the resilience of patients and improve their disease self-management by formulating relevant intervention strategies, thereby effectively reducing their fear of progression.

Objective:To explore the status and influencing factors of stigma in patients with pituitary adenoma.Methods:From January 2020 to May 2021, a total of 106 patients with pituitary adenoma in the Affiliated Huai'an No.1 People 's Hospital of Nanjing Medical University were selected using the convenience sampling method. Internalized Stigma of Mental Illness, Medical Coping Style Questionnaire and Connor-Davidson Resilience Scale were used to investigate them. Binary Logistic regression analysis was used to evaluate the influencing factors of stigma in patients with pituitary adenoma. Results:Among 106 patients with pituitary adenoma, 87 (82.08%) had stigma. The stigma score of patients with pituitary adenoma was (2.58±0.61) . Binary Logistic regression analysis results showed that family monthly income, coping style and mental elasticity were the influencing factors of stigma in patients with pituitary adenoma ( P<0.05) . Conclusions:Stigma is common in patients with pituitary adenoma. Medical staff should take targeted intervention measures according to the influencing factors of stigma in patients with pituitary adenoma, so as to help patients reduce stigma.

BACKGROUND:In recent years,epidemiological studies have shown that sleep patterns are risk factors for osteoarthritis,but the causal relationship between sleep characteristics and osteoarthritis remains unknown. OBJECTIVE:To investigate the causal relationship between seven sleep phenotypes and osteoarthritis,thereby providing a theoretical foundation for clinical prevention and intervention of osteoarthritis. METHODS:Seven sleep-related features,namely sleep duration,wake-up time,daytime napping,morning/evening preference,snoring,insomnia,and hypersomnia,were selected from published genome-wide association studies.Instrumental variables for these sleep-related features were extracted.Instrumental variables for knee osteoarthritis and hip osteoarthritis were obtained from publicly available genome-wide association studies.Causal relationships between sleep characteristics and outcome risks were evaluated using two-sample and multivariable Mendelian randomization analyses.The inverse variance weighted method was employed as the primary Mendelian randomization approach.Various methods,including weighted median,weighted mode,Mendelian randomization-Egger regression,Mendelian randomization pleiotropy-residual sum and outlier,were utilized to detect and correct for the presence of pleiotropy. RESULTS AND CONCLUSION:The results of the inverse variance-weighted method in the two-sample Mendelian randomization study revealed a detrimental causal association between the duration of sleep and the incidence risk of knee osteoarthritis[odds ratio(OR)=0.621,95%confidence interval(CI):0.470-0.822,P=0.001].Concurrently,insomnia displayed a positive causal connection with hip osteoarthritis risk(OR=2.016,95%CI:1.249-3.254,P=0.005).Sensitivity analysis affirmed the robustness of these causal relationships,and Mendelian randomization-Egger intercept analysis found no evidence of potential horizontal pleiotropy(knee osteoarthritis:P=0.468,hip osteoarthritis:P=0.551).Moreover,the results from the multivariable Mendelian randomization analysis showed that the causal association between insomnia and hip osteoarthritis lacked statistical significance(P=0.715).In contrast,sleep duration exhibited a direct negative causal relationship with the incidence risk of knee osteoarthritis(OR=0.526,95%CI:0.336-0.824,P=0.005).Reverse Mendelian randomization analysis indicated that knee osteoarthritis did not influence sleep duration(P=0.757).These findings indicate a negative correlation between sleep duration and incidence risk of knee osteoarthritis,suggesting that correcting insufficient sleep might mitigate the incidence risk of knee osteoarthritis.

Knee osteoarthritis （KOA） is a common degenerative joint disease characterized primarily by the degeneration and damage of knee joint cartilage， accompanied by osteophyte formation and inflammation. In recent years， the prevalence of KOA has been increasing globally， significantly impacting the quality of life patients. However， the pathogenesis of KOA remains not fully understood， and current treatment methods are limited. Therefore， finding new therapeutic strategies is a research hotspot. Previous studies have found that the onset of KOA is related to abnormal mitochondrial regulation. Mitochondria， functioning as secondary messengers， play crucial roles in cellular respiration， reactive oxygen species （ROS） generation， and adenosine triphosphate （ATP） production through oxidative phosphorylation. Mitochondrial quality control is a pivotal mechanism for maintaining the morphology， quantity， and quality of mitochondria. The connection between mitochondrial quality control and the pathogenesis of KOA involves several factors， such as mitochondrial oxidative stress， mitophagy， imbalances in mitochondrial biogenesis， abnormal mitochondrial dynamics （fission and fusion）， and dysregulation of calcium ions. Metabolic abnormalities in the body lead to mitochondrial structural damage， which in turn contributes to the onset and progression of KOA. Traditional Chinese medicine （TCM） has made some progress in intervening in mitochondrial quality control， employing multi-faceted， multi-pathway， and multi-target strategies to treat KOA. Several studies have shown that mitochondrial quality control may be one of the therapeutic targets of TCM in treating KOA. However， there is currently a lack of comprehensive reviews summarizing the TCM interventions in mitochondrial quality control for treating KOA. This paper systematically reviewed the research progress in TCM treatment of KOA based on five aspects of mitochondrial quality control， aiming to provide a theoretical basis for the clinical prevention and treatment of KOA.