Objective To understand the prevalence and epidemiological features of diabetes mellitus (DM ) in Nan′an district of Chongqing ,and to investigate the relationship between blood glucose with various factors of sex ,age and body mass index(BMI) to provide the basis for prevention and treatment of DM .Methods Take the local resident population as the investigation object ,By u-sing the epidemiological investigation method of random sampling ,the native permanent residents were taken as the respondents ,the epidemiological survey including the general conditions (gender ,age ,occupation and nationality ) ,height ,weight ,waist circumfer-ence ,waistline ,blood pressure ,heredity family history ,environmental factors ,personal dietary habits and social psychological factors was performed .Results Among 10 932 permanent residents ,DM was found in 447 cases ,the prevalence rate was 4 .09% ,in which the prevalence rate of females was significantly higher than that of males ;the prevalence rate of DM showed an upward trend with the age increase ;the incidence rate of DM in the obesity population was obviously higher than that in the normal population .The people with smoking history ,hypertension ,dyslipidemia have higher incidence rate than the common populations .Conclusion DM is a disease closely related with the factors of sex ,age ,obesity ,smoking ,hypertension and dyslipidemia .Early intervention control has important significance .

Objective To obtain monoclonal antibodies(McAb) against human IgE by recombinant IgE antigen.Methods BLAB/c mice were immunized with recombinant IgE.The spleen cells of immunized mice were used to prepare the McAb by hybridoma techniques.IgE-McAb was selected by indirect ELISA with culture supernatant of hybridoma cells.ELISA blocking assay were employed to identify the function of McAb.Alanine scanning mutagenesis were used to confirm the antigenic epitopes of McAb.Results A monoclonal antibody against IgE(178) was produced.The antigenic epitope of 178 was found to be spatially close to the receptor-binding site.Functional assay revealed that 178 could markedly inhibit IgE binding to receptors.Conclusion A hybridoma cell line secreting IgE-McAb stably was obtained by recombinant IgE antigen,and the IgE-McAb might block IgE binding to receptors via steric hindrance.