0.05),and with significant differences as the TCM group and the WM group compared with TCM plus WM group(P

Sepsis,a complex clinical syndrome resulting from a harmful and damaging host response to infection, is the leading cause of mortality in intensive care units.Early diagnosis and appropriate intervention can improve the prognosis of patients with sepsis.Many studies have confirmed that sepsis at different stages is in different immune status.Priority used to be given to systemic inflammatory response, but immune-suppression has become the focus of study. Immune-suppression and secondary infection are the major causes of death of patients with sepsis.Study of sepsis is shifting to immune-suppression and its regulation mechanisms.

Endoscopic retrograde cholangiopancreatography (ERCP)has become an important method for the diagnosis and treatment of cholangio -pancreatic duct diseases.Post -ERCP pancreatitis (PEP)is the most common complication and its incidence has increased up to 40% in recent years.The prevention of PEP has always been a hot topic in clinical research.This article reviews the research advances in the pharmacological prevention of PEP and points out that controlled clinical trials with a large sample size are still needed to investigate the effect of preventive drugs,and further studies should focus on optimal medication time,route of administration,and dose.In addition,de-mographic features of populations from different countries and different age groups should be taken into consideration.

Objective To study the influence of oral antigens on the expression of costimulatory molecules CD80/CD86 on the surface of M and to investigate the genesis of pregnancy immunological tolerance induced by oral antigens through CD80/CD86. Methods Mice of spontaneous abortion(CBA/J?DBA/2) were divided into two groups: immune group and non-immune group.Trophoblast membrane antigen-2(TMA2)or ovum albumin(OVA) was administered orally to the mice in the immune group.Mice of normal pregnancy(CBA/J?BALB/c) were served as controls.The number of CD80/CD86 M of spleen and mesenteric lymph node(MLN) were determined by double-label FCM method. Results In the MLN,CD80 M of non-immune group was increased significantly as compared with that of the control group(P

Objective To investigate the value of serum?-human chorionic gonadotropin(?-HCG) and progesterone monitoring in medicinal conservative treatment for ectopic pregnancy. Methods Sixty patients with ectopic pregnancy treated with methotrexate were divideded into group A(n=39,?-HCG0.05).The time of serum progesterone level declining to normal level was significantly shorter than that of ?-HCG in both group A and group B(P

0.05). Conclusion:Ti-29Nb-13Ta-4.6Zr alloy is histocompatible.

Objective To explore the effects of different infants-feeding patterns on preschoolers' physical development, intelligence development and bone mineral density (BMD). Methods A total of 953 preschoolers from seven cities and two rural areas in China were recruited by multiple stage stratified cluster sampling methods from November 2011 to April 2012. Physiology measurement was performed to get their height and weight and to calculate body mass index(BMI). BMD of distal radius was measured by ultrasound BMD scanner. The intelligence quotient was assessed by Standford-Binet Test of Intelligence(Chinese Version). The demographic factors and the feeding ways were investigated by an interviewer-administered questionnaire and all subjects were divided into three groups : exclusive breastfeeding, mixed feeding and artificial feeding group. ANOVA analysis, Chi-square test and non-parametric test were used to analyze the data. Results There were 476, 335 and 142 children in exclusive breastfeeding, mixed feeding and artificial feeding groups, respectively. The exclusive breastfeeding rate in urban areas was significantly lower than that in rural areas [47.2%(343/726) vs 58.6%(133/227), χ2=9.780, P=0.008]. The height-for-age Z score and weight-for-age Z score were significantly different among children with different feeding ways (χ2=10.728 and 12.175, P=0.005 and 0.002). The children in the exclusive breast feeding group showed lower height-for-age Z score and lower weight-for-age Z score comparing with those in the mixed feeding and artificial feeding groups (all P<0.05). The value of speed of sound of BMD was significantly different among exclusive breastfeeding, mixed feeding and artificial feeding groups [(3 640.2±142.0) vs (3 613.9±141.9) and (3 613.4±143.0) m/s, F=3.946, P=0.020], and that in the exclusive breastfeeding group was higher than in the mixed feeding group (P<0.05). No significant difference was found in BMI-for-age Z score, intelligence score and food allergy rate and food intolerance rate among the three groups(all P>0.05). Conclusions Feeding patterns during the first several months after birth may affect the weight, height and BMD in preschool-children, but not the intelligence development of these children.

Objective To compare the phenotype of myeloid derived suppressor cells (MDSCs) separated from the bone marrow of mice 3 d and 7 d after cecal ligation and puncture ( CLP) and to elucidate its potential role in the accumulation and immuno-function of MDSCs by determining the expression of microRNA-146a(miR-146a)in order to explore the effect of miR-146 a on immonosuppression of MDSCs in sepsis .Methods A septic model was prepareol by CLP in adult male C57BL/6J mice.MDSCs(expressing cell-surface CD11b and GR-1 antigens )from bone marrow were harvested 3 and 7 days after CLP and were separated with magnetic bead sorting technique .Then,cytokines secretion and arginase-I activity were detected and the T cell proliferation in vitro and the expression of miR-146a of MDSCs (3 d and 7 d after CLP)were observed.Results MDSCs secreted mostly such promoting inflammatory factors as TNF-α, IL-6 3 days after CLP, but 7 days after CLP , they primarily secreted IL-10 and TGF-βwhich were anti-inflammatory factors . MDSCs had potent immunosuppressive properties by increasing T cell suppression in a late anti-inflammatory phase ( CLP3 d vs CLP7 d, P<0.05).In the meantime,miR-146a of the MDSCs in bone marrow was overexpressed in septic mice at 7 days(P<0.05). Moreover,the expression of miR-146a of the MDSCs in bone marrow of septic mice was higher at 7 days than at 3 days after CLP(P<0.05).Conclusion The data indicate that the phenotype of MDSCs evolves through early pro -inflammatory phase into the late anti-inflammatory phase .MDSCs have potent immunosuppressive properties in the late phase of sepsis . miR-146 a might play a crucial role in the regulation of immunosuppressive activity of MDSCs in late sepsis .

Objective To investigate the effect of infusion of sodium bicarbonate in amniotic cavity and exchange of amniotic fluid for fetus with distress and acidosis. Methods The patients included 40 cases of oligohydramnios with mild and serious abnormality of fetal heart rate and amniotic fluid contamination of degree Ⅱ or more during the labor. The 40 cases had exchange of amniotic fluid with infusion under continuous monitoring. Twenty of them had infusion with 5% sodium bicarbonate into amniotic cavity; the other 20 cases received 5% sodium bicarbonate intravenous in fusion. After the labor all the patients had test of arterial blood gas in umbilical cord and the fetuses were evaluated with Apgar score. Results (1)the effective rate was 88% in the group of infusion into amniotic cavity and 85% in the group of exchange of amniotic fluid. (2)The arterial blood pH, PO_2, HCO~-_3, ABE, SBE in the group of amniotic cavity infusion with 5% sodium bicarbonate were all higher than group of Ⅳinfusion, however PCO_2 was significantly lower than the group of Ⅳ(P

Objective To observe the change regularity of IL-1?, IL-6, IL-10 and explore the mechanism of Yingfen syndrome (YS) in epidemic febrile diseases. Methods One hundred and four cases were divided into acute leukemia (AL) group and non-acute leukemia (NAL) group according to different diseases. According to syndrome differentiation of TCM, AL group was classified to 10 cases for Weifen syndrome (WS), 12 cases for Qifen syndrome (QS), and 20 cases for YS. NAL group was classified to 20 cases for WS, 24 cases for QS and 18 cases for YS. Fifteen healthy persons were selected for comparison. The levels of IL-1?, IL-6 and IL-10 in YS, WS and QS of various diseases were observed. Results In YS period, IL-1? and IL-6 increased continuously, while IL-10 decreased markedly, compared with healthy people (P 0.05), while on difference in IL-6, IL-10 between different diseases (P