Objective To investigate the effects of Modifded DaHuang ZheChong Pill on T lymphocyte subpopulation of S180 tumor-bearing mice.Methods The mice were built to the S180 sarcoma model.The experimental animals were divided into 5 groups:High-dose Modifded DaHuang ZheChong Pill group.middle-dose Modifded DaHuang ZheChong Pill group,low-dose Modifded DaHuang ZheChong Pill group,model group,and normal group.The proportion of the T lymphocyte subpopulation was detected by Flow Cytometer in the different experimental groups.Results Modifded DaHuang ZheChong pill could control radio of CD4/CD8,especially,in the high dosage group.Conclusion Modifded DaHuang ZheChong Pill could remit and improve the immune function in tumor-bearing mice,showing the best results in High-dose Modifded DaHuang ZheChong Pill group.

The property theory of Chinese herbal medicine (CHM) is regarded as the core and basic of Chinese medical theory, however, the underlying mechanism of the properties in CHMs remains unclear, which impedes a barrier for the modernization of Chinese herbal medicine. The properties of CHM are often categorized into cold and heat according to the theory of Chinese medicine, which are essential to guide the clinical application of CHMs. There is an urgent demand to build a cold/heat property classification model to facilitate the property theory of Chinese herbal medicine, as well as to clarify the controversial properties of some herbs. Based on previous studies on the cold/heat properties of CHM, in this paper, we described a novel strategy on building a cold/heat property classification model based on herbal bio-effect. The interdisciplinary cooperation of systems biology, pharmacological network, and pattern recognition technique might lighten the study on cold/heat property theory, provide a scientific model for determination the cold/heat property of herbal medicines, and a new strategy for expanding the Chinese herbal medicine resources as well.
Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Herbal Medicine ; Humans ; Phytotherapy ; Plants, Medicinal ; chemistry

Objective: To explore the effect of activation of peroxisome proliferator-activated receptor gamma (PPARγ) on cell cycle arrest of gastric carcinoma cell line MGC803. Methods: The inhibitory effect of pioglitazone (PGZ) on proliferation of MGC803 cells was analyzed by MTT assay. Cell cycle were detected by flow cytometry (FCM). The protein expression of PPARγ, cyclinD1 and cell cycle protein-dependent kinase CDK4 in MGC803 cells was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Treatment with 0.1-10 μmol/L, PGZ for 96 h significantly inhibited cell proliferation. The proportion of MGC803 cells at G1 phase was significantly increased when treated with 10 μmol/L PGZ for 48, 72 and 96 h, and showed an apparent G1 phase arrest. The expression of PPARγ was at a low level in MGC803 cells and significantly up-regulated when treated with 10 μmol/L, PGZ for 48 h (P < 0.01). The expression of CDK4 in MGC803 cells was significantly down-regulated when treated with 10 μmol/L, PGZ for 96 h (P < 0.01) and the expression of cyclinD1 was slightly down-regulated. Conclusion: Activation of PPARγ significantly induces G1 phase arrest, which is associated with down-regulation of the expression of CDK4 and cyclinD1.

AIM: To observe the clinical efficiency of intravitreal conbercept on diabetic macular edema(DME).METHODS: This was a single arm, open-babel prospective study.Twenty eyes from 20 patients (12 males and 8 females) with DME diagnosed by fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) were enrolled.Before the injection, best-corrected visual acuity (BCVA) of early treatment of diabetic retinopathy study (ETDRS), non-contact tonometer, ophthalmoscope, fundus photography, fundus fluoresein angiograph (FFA), and OCT were examined.All affected eyes were treated with intravitreal conbercept 0.05mL (10mg/mL).Patients were followed up for 6 to 11mo, with a mean duration of 8.55±1.96mo.Post-treatment BCVA, CMT, leakage of macular edema and complications were compared with baseline using repeat analysis.RESULTS: The initial average visual acuity (ETDRS letters) were 43.35±17.45, range from 9 to 70.The initial average central macular thickness (CMT) was 576.30±167.92μm, range from 337 to 987μm.The mean BCVA showed significant improvement during 1, 3, 6mo post-treatment and the latest follow up, with a mean increase of 11.2±5.9, 13.8±7.9, 15.7±6.8 and 14.7±8.6, respectively (P<0.01).The changes of BCVA between before and at 1mo after treatment were different compared with the changes between before and at 6mo (P<0.01).During the latest follow up, the mean BCVA was obviously improved in 10 eyes (50%), improved in 7 eyes (35%), stable in 3 eyes (15%).Likewise, the mean CMT significantly decreased during the follow-up period with a mean CMT reduction of 183.8±159.5, 292.9±169.0, 271.4±167.2 and 286.4±166.9μm respectively (P<0.001).The CMT at 1mo were different with that 3, 6mo and final follow-up (P<0.01).During the latest follow up, macula lutea leakage disappeared in 6 eyes (30%), decreased in 12 eyes (60%) and increased in 2 eyes (10%).No adverse events such as secondary retinal detachment or endophthalmitis were found during the follow-up.CONCLUSION: Intravitreal conbercept significantly improve visual acuity and macular edema exudation.

● AIM: To investigate the sequential changes of HIF-1 α Protein and mRNA in hypoxic bovine retinal microvessel endothelial cells.● METHODS: The bovine retinal microvessel endothelial cells were cultured in normoxic and CoCl2-induced hypoxic conditions respectively. Expressions of HIF-1 α Protein were measured with immunohistochemical staining, and RT-PCR was used to determine the HIF-1 α mRNA.● RESULTS: HIF-1 α began to increase 1h after hypoxia,and reached the peak at 4h. After 16h, it declined significantly. Compared with the normoxic group, the expression of HIF-1 α protein in the hypoxic groups had significant difference (P＜0.01), and HIF-1 α mRNA expression was unchanged under hypoxia.● CONCLUSION: HIF-1 α participates in the hypoxic procedures in retinal microvessel endothelial cells, and hypoxia induce time-dependent changes of HIF-1 α protein expression, which is not modulated on the transcription level. Analysis of HIF-1 α expression revealed a temporal and spatial changes with regard to the hyperoxic repression, indicating that HIF-1 may play a major role in the development of retinopathy of prematurity and other ischemic retinal disorders such as diabetic retinopathy.

Child ; Humans ; Myelodysplastic Syndromes ; diagnosis ; Prognosis

Periprosthetic osteolysis and aseptic loosening after total hip arthroplasty are the common causes for revision surgery,which are initiated by wear particles released from the articular surfaces of prosthetic components.These components include ultra-high molecular weight polyethylene,titanium alloy,Al2O3,ZrO2 and polymethylmethacrylate.Wear particles activate macrophage via phagocytosis or pattern recognition receptor,resulting in release of inflammatory mediators such as macrophage colony-stimulating factor (M-CSF),macrophage chemotactic protein-1 (MCP-1),macrophage inhibitory protein-1 (MIP-1),IL-1β,IL-6 and TNF-α.Furthermore,wear particles stimulate M0 macrophage to polarize to M1 macrophage,promoting the release of inflammatory mediators.The periprosthetic osteolysis process is a cell/cytokine-mediated biological cascade induced by macrophage activation,involving the monocyte/macrophage cell line,such as macrophages,osteoclasts,and dendritic cells.The mesenchymal cells,including osteoblasts,osteocytes,fibroblasts and lymphocytes,lead to a deviation in the balance of osteoblast-osteoclast interactions within the basic multicellular unit,resulting in periprosthetic osteolysis.Fibrobalst,dendritic,and lymphocyte cells can enhance the recruitment and activation of macrophages by secreting MCP-1,MIP-1,and IL-8.Furthermore,the upregulated expression of RANKL,TNF-α,and IL-1 β facilitates the osteoclast differentiation from osteoclast precursor.TNF-α,IL-1 β together with wear particles can upregulate the expression of IL-6,MCP-1 and M-CSF of osteoblast and promote the recruitment and activation of macrophages.Nuclear transcriptional factor NF-κB plays a vital role in mediating periprosthetic inflammatory response and gene expression of bone metabolism.Different inflammatory factors can induce different expression of its downstream gene,ultimately,promoting or hindering the osteolysis process.The individual difference of periprosthetic osteolysis may also relate to single nucleotide polymorphism of IL-1RA,IL-6 and MMP-1 gene.

BACKGROUND:Pericytes, which are widely distributed in the microvasculature except lymphatic vessels, are not only a constituent of microvessels, but also play an important role in microvascular occurrence, development, stability, maturity and remodeling. Its signal transduction is currently a hot spot. OBJECTIVE:To review recent advances in the signal pathways related to pericytes and angiogenesis. METHODS:We retrieved PubMed database, Wanfang database, CNKI database and China Biology Medicine disc for articles addressing pericytes and angiogenesis published from 1994 to 2014. The keywords were “pricytes; endothelial cels; microvessels; signal transduction” in English and Chinese, respectively.RESULTS AND CONCLUSION:In the early stage of capilary formation, recruitment of pericytes contributes to the occurrence and development of new blood capilaries. However, in the late stage of angiogenesis, pericytes inhibit endothelial cel proliferation and promote endothelial cel differentiation, thereby promoting vascular maturation, maintaining normal structure and regulating their permeability. In this process, signaling pathways of platelet-derived growth factor B/platelet-derived growth factor receptor β, transforming growth factor β, angiopoietin-1/Tie-2, human heparin binding epidermal growth factor/ErbBs, and stromal cel derived factor 1α/CXCR4 play an important regulatory role in pericytes and angiogenesis.

Objective To analyze the clinical efficacy and safety of amiodarone in the treatment of the elderly patients with ventricular arrhythmia.Methods 136 elderly patients with ventricular arrhythmia were randomly divided into the observation group (n =68cases) and control group (n =68cases).The observation group was given amiodarone by intravenous to treat the elderly ventricular arrhythmia,and patients in the control group were treated with combination therapy.After treatment for 4 weeks,the treatment outcomes were observed and compared.Results The obvious effective rate and total effective rate of the observation group was 51.47％,92.65％).which were significantly higher than those in the control group (51.47 ％,92.65 ％) (P ＜ 0.05).There was no significant difference in the incidence rate of adverse effect between the two groups (P ＞ 0.05).Conclusion Amiodarone has good clinical effect in treatment of elderly patients with ventricular arrhythmia,it is worthy of clinical use.

Objective To investigate the value of TAP in the early prediction of severe acute pancreatitis (SAP) and to compare it with acute physiology and chronic health evalutionⅡ (APACHEⅡ).Methods We observed the association between TAP concentration and the severity of acute pancreatitis with competitive enzyme-linked immunosorbent assay(ELISA). Urine samples were collected for the measurement of TAP concentration at admission, 24,48,and 72*!h after from 41 patients with acute pancreatitis (AP) (12 severe cases, and 29 mild) who presented within 48*!h of the onset of symptoms and from 11 control patients, while APACHEⅡ scores were recorded at 48*!h after admission. Results The TAP at admission of SAP (95*!nmol/L) was signficantly higher than that of mild (20*!nmol/L; P