2.In-vitro and in-vivo evaluation of the antibacterial potential of Typha elephantina
Ahmad, B. ; Yousafzai, A.M. ; Zeb, A. ; Khan, A.A. ; Attaullah, M. ; Ahmad, S.
Tropical Biomedicine 2021;38(No.1):1-7
The present study was aimed to evaluate the in-vitro and in-vivo antibacterial effects of the
Typha elephantina aqueous extract (TE.AQ), ethanolic extract (TE.ET) and T. elephantina methanolic
extract (TE.ME) against eight selected clinical pathogens. The test samples were tested for
in-vitro analysis (by disc diffusion method) at different concentrations of 5, 15, 25, 50 and 100
mg/dL against both gram positive and gram-negative strains. The highest potential was
observed in TE.ME at a concentration of 100 mg/dL against Pseudomonas aeruginosa exhibiting
19.67 ± 0.577 mm zone of inhibition (ZOI). The same fraction also showed good activity
against Staphylococus aureus with ZOI of 17.50 ± 0.70 mm. The TE.ET was found most active
against P. aeruginosa and Streptococcus pyogenes having ZOI of 18.53 ± 0.503 and 16.2 ± 1.55 mm
respectively at a concentration of 100 mg/dL. The most sensitive bacteria P. aeruginosa was
selected for in-vivo study (using poultry chicks) for induction of infection in chicks. The effects
of TE.AQ, TE.ET and TE.ME were determined at concentrations of 300 mg/kg body weight
based on hematological parameters, liver enzymes and gross pathological findings of lungs
and livers. The findings of the in-vivo study in chick’s model showed that treatment of
experimental animals with TE.ME significantly restored the hematological parameters, liver
enzymes and architecture of lungs and livers. Based on scientific evidence, the current study
suggests that TE.ME may serve as a best and new natural antibacterial agent and can be
used against infections caused by P. aeruginosa.