BACKGROUND: Detection of anti-Kidd antibody is important because of its clinical significance. If detection is difficult due to weak serological reactivity or dosage effect, use of an enzyme method could be helpful. However, despite use of an enzyme method, we still observed weak reactivity of anti-Kidd antibody. METHODS: All identified anti-Kidd antibody cases from Jan 2012 to Aug 2015 in Asan Medical Center were reviewed. Antibody identification test was performed using the column agglutination technique using Bio-Rad ID-DiaPanel with LISS/Coombs card, Bio-Rad ID-DiaPanel-P with NaCl/Enzyme card, and ID-DiaPanel-P with LISS/Coombs card. The test results were compared. RESULTS: Sixty cases of anti-JK(a) or anti-Jk(b) were detected and tested by enzyme method. Among them, 34 (56.6%) cases showed strengthened reactivity using the ID-DiaPanel-P with NaCl/Enzyme card method. However, 26 (43.4%) cases showed weakened reactivity. Of these, 13 cases that could be tested by an additional method using ID-DiaPanel-P with LISS/Coombs card containing anti-IgG and anti-C3d showed successfully strengthened reactivity. CONCLUSION: The reactivity of anti-Kidd antibodies that was not strengthened using ID-DiaPanel-P with NaCl/Enzyme card method could be successfully strengthened by use of the ID-DiaPanel-P with LISS/Coombs card.
Agglutination ; Antibodies* ; Chungcheongnam-do

The study carries on 87 E.coli strains. Using reversed passive latex agglutination (RPLA) technique to discover factor causing disease EspB to confirm EPEC. The result: 27 strains have no gene eae, 52 strains has eae possitive, 19 of them has Stx. In fact, following traditional, the rate infected EPEC was determined essentialy relying on slide agglutination reaction with antiserum group O of EPEC. This experience showed that the factor causing disease EspB in order to determine EPEC can apply at clinical laboratory or in community, although, most ETEC extract also EspB, Sxt and can also be determined in similar culture. This method is necessary need and significance in fact
Escherichia coli ; diagnosis ; Agglutination

BACKGROUND: The use of the microcolumn agglutination method for red cell antibody screening and identification is on the increase because it has several advantages over the conventional tube method. The aim of this study was to compare two microcolumn agglutination systems, the Ortho BioVue (Ortho-clinical Diagnostics, Amershman, Bucks, UK) and the DiaMed-ID (DiaMed Ag, Cressier, Morat, Switzerland), which are both popularly used in Korea. METHODS: We used 897 consecutive serum samples that were requested to undergo red cell antibody screening. They were collected from February, 2008 to March, 2008 at Seoul National University Boramae Hospital. All the serum samples were screened for red cell antibody by both microcolumn agglutination systems, and any positive sample by either of the two systems was re-tested for antibody identification by both systems. We followed the instructions of each manufacturer and we used the LISS/Coombs microcolumn agglutination method for red cell antibody screening and identification. RESULTS: The rate of positive screening was 0.8% by the Ortho BioVue and 0.7% by the DiaMed-ID with insignificant differences between the two systems (P=0.439). The two systems showed excellent overall concordance in screening, 99.4%. Among the 9 samples with positive screening results, we found specific antibodies in only four samples. The rate of identification was 29% (2/7) by the Ortho BioVue and 33% (2/6) by the DiaMed-ID. CONCLUSION: Both methods were very comparable on performing red cell antibody detection and identification. Thus, they could both be used in laboratories for routine tests in such a way as to compensate for any shortcomings of the other method.
Agglutination ; Antibodies ; Mass Screening

BACKGROUND: The use of the microcolumn agglutination method for red cell antibody screening and identification is on the increase because it has several advantages over the conventional tube method. The aim of this study was to compare two microcolumn agglutination systems, the Ortho BioVue (Ortho-clinical Diagnostics, Amershman, Bucks, UK) and the DiaMed-ID (DiaMed Ag, Cressier, Morat, Switzerland), which are both popularly used in Korea. METHODS: We used 897 consecutive serum samples that were requested to undergo red cell antibody screening. They were collected from February, 2008 to March, 2008 at Seoul National University Boramae Hospital. All the serum samples were screened for red cell antibody by both microcolumn agglutination systems, and any positive sample by either of the two systems was re-tested for antibody identification by both systems. We followed the instructions of each manufacturer and we used the LISS/Coombs microcolumn agglutination method for red cell antibody screening and identification. RESULTS: The rate of positive screening was 0.8% by the Ortho BioVue and 0.7% by the DiaMed-ID with insignificant differences between the two systems (P=0.439). The two systems showed excellent overall concordance in screening, 99.4%. Among the 9 samples with positive screening results, we found specific antibodies in only four samples. The rate of identification was 29% (2/7) by the Ortho BioVue and 33% (2/6) by the DiaMed-ID. CONCLUSION: Both methods were very comparable on performing red cell antibody detection and identification. Thus, they could both be used in laboratories for routine tests in such a way as to compensate for any shortcomings of the other method.
Agglutination ; Antibodies ; Mass Screening

BACKGROUND: Type and screen is recommended for efficient use of blood and reduction in workload in blood bank. Column agglutiation test is standardized and easy to perform, and provide clear and stable reactions that improve the interpretation of results. In this study, we compared column agglutination test(Ortho Diagnostic Systems Inc., USA) with conventional tube test and investigate its usefulness in irregular antibody screening and identification. METHODS: A total 182 samples were screened for irregular antibodies using column agglutination test and conventional tube test. And 18 patient's sera in which irregular antibodies previously screened by both tube and column agglutination tests were identified for irregular antibodies by tube and column agglutination tests. We compared the results of two tests. RESULTS: In the screening test, there was 96.7%(176/182) agreements between column agglutination test and conventional tube test. The column agglutination test showed stronger reactivity than tube test. In the irregular antibody identification, there was 88.8%(16/18) agreement between two tests and disagreement were seen in the identification of anti-P1 and anti-Leb antibodies. CONCLUSION: The results of column agglutination test are objective and superior to the conventional tube test in irregular antibody screening and identification tests. These results suggest that the column agglutination test will be useful and more convenient test in antibody screening and identification.
Agglutination Tests* ; Agglutination* ; Antibodies ; Blood Banks ; Mass Screening*

We report a case of Ael in a 44-year old woman. The patient' red cells were typed as O and her serum had both anti-A and anti-B, but the agglutination strength with A1 cell was weaker (2+) than with B cell (4+) in her serum. Additional tests showed that the red cells were not agglutinated by anti-A,B and A antigen on patient' RBC was demonstrated by adsorption-elution test. Her saliva contained H but no A substance, and the ABO genotyping test identified her blood type as AO. We concluded that this was a case of blood type Ael with anti-A.
Adult ; Agglutination ; Female ; Humans ; Saliva

No abstract available.
Agglutination* ; Animals ; Cats* ; Erythrocytes* ; Humans*

BACKGROUND: There is a recent trend of increased use of the gel test for detecting unexpected antibodies because of its simplicity and the ease with which a definitive interpretation can be made. The aim of the present study was to evaluate the diagnostic performance of newly developed DG Gel microtube column agglutination system as compared with the tube method and other two microtube column agglutination systems (DiaMed-ID, Ortho BioVue). METHODS: We collected 305 patients who were screened for unexpected antibody from February to July 2007. These samples were screened and we identified the unexpected antibody with the tube method and three microtube column agglutination systems. RESULTS: The highest estimated sensitivity of the screening test was 97.4% for DiaMed-ID. The highest estimated specificity of the screening test was 100% for DG Gel. The least number of discordant identification results was seven for the DG Gel. CONCLUSION: DG Gel has good diagnostic efficacy and accuracy for identifying unexpected antibody. DG Gel might be used as a replacement or supplement for the previous tests.
Agglutination ; Antibodies ; Humans ; Mass Screening ; Sensitivity and Specificity

Methods, data, and discussions of experiments concerning agglutination of human leukemic leukocytes with guinea pig serum and the alkaline phosphatase staining technique by the Azo-dye method are given. An important fact evident from studies of alkaline phosphatase is that there can be chemical differences between cells whose morphology is identical. Even though the results obtained here in the small group of leukemic cases studied by the guinea pig serum agglutination method are not very reproducible, the idea that an antigen-antibody reaction for the determination of leukemia exists, very appealing.
Agglutination ; *Alkaline Phosphatase ; Leukemia/*diagnosis ; *Leukocytes

Background: Only strains of V cholerae 01 that produce cholera toxin have been associated with epidemics and pandemics in the past; therefore, production of cholera toxin has become an important marker for identifying isolates with the potential to cause epidemics. The Reversed Passive Latex Agglutination test (RPLA) is a semi-quantitative determination of CT or LT in culture fluid. \r\n', u'Objective: To determine the cholera toxin by RPLA in order to evaluate the severity of the epidemic and find an interventional solution.\r\n', u'Subjects and methods: The technique of RPLA enables soluble antigen such as bacterial toxins to be detected in an agglutination assay. A total of 44 strains of V. cholerae 01 were tested for the production of the cholera toxin by RPLA.\r\n', u' Results and conclusion: The concentration of cholera toxin was determined from strains isolated in 2007 much higher than that from the strains isolated in 2004. These results can explain the severe cholera endemic in 2007 in comparison with the endemic in 2004. The RPLA test is a simple and reliable method for determining cholera toxin and suitable for epidemiologic studies on cholera. \r\n', u'
Reversed passive latex agglutination ; cholera toxin