1.Viability and DNA Damage of Buccal Mucosa Cells in Patients Exposed to Panoramic X-ray
Ryna Dwi Yanuaryska ; Afit Aditya Atmoko ; Isti Rahayu Suryani ; Rurie Ratna Shantiningsih
Archives of Orofacial Sciences 2021;16(SUPP 1):43-49
ABSTRACT
Panoramic X-ray is well known to cause DNA damage and induces cellular death. The aim of the
present study was to evaluate the cytotoxicity of radiation exposure from panoramic radiography on
human buccal mucosa cells by assessing the cell viability using the simple-trypan blue exclusion test.
The genotoxicity effect was evaluated by assessing comet assay score. This research included a total of
20 healthy patients who had panoramic radiography for a routine dental examination. Buccal mucosa
cells were collected from all participants before X-ray exposure and at 30 min or 24 h after exposure in
Groups 1 and 2, respectively, and subjected to a comet assay and trypan blue exclusion test to assess
cell viability and DNA damage. Cell viability was calculated as the ratio of live (translucent) to total
counted cells. Comet assay output images were analysed using OpenComet software and a visual score
by measuring the percentages of tail DNA and summing the visual score, respectively. A statistically
significant (p < 0.05) reduce in cell viability was observed at 30 min after exposure, furthermore there is
no more reduction after 24 h. Both comet assay measurements showed a significant (p < 0.05) increase
in the percentage of tail DNA and visual score at 30 min after exposure, then tend to decrease after 24 h
of exposure, although it was not significant (p > 0.05). The results showed that panoramic radiography
interfered cell viability and induced DNA damage in buccal mucosa cells within 30 min after exposure,
but these effects were ceased after 24 h.
Mouth Mucosa--cytology
;
Radiography, Panoramic