This study aims to determine the knowledge, attitudes and ergonomic practices (KAP) of office workers in Malaysia in preventing work-related musculoskeletal disorders. An online crosssectional survey of 266 office workers in Malaysia was conducted. All participants were asked to complete a five-part questionnaire to extract information on sociodemographic data, prevalence of work-related muscle disorders (WMSD), level of knowledge, attitudes, and ergonomic practices. The prevalence of WMSD was measured using the Cornell MS Discomfort Questionnaire (CMDQ) with high reliability and validity, with a Cronbach's Alpha value exceeding 0.95, and an intraclass correlation coefficient (ICC)= 0.69-0.95. Ergonomic knowledge was measured using the Ergonomic Knowledge Questionnaire with high validity (0.98) and fair reliability (r= 0.75). The attitude questionnaire was measured using a Likert scale consisting of yes, no, and maybe. Ergonomic practices are obtained by using the Rapid Office Strain Assessment (ROSA). ROSA is a questionnaire with high inter-observer and intraobserver reliability (ICC 0.88 and 0.91). Participants answered a picture-based questionnaire consisting of 3 parts: (1) chair, (2) monitor and telephone, (3) mouse. Most office workers experienced WMSD (99.3%) with moderate discomfort (66.5%). The common complaints being upper back (83%), right upper arm (78.9%) and neck (78.6%). A total of 73.7% of office workers have poor and little knowledge about ergonomics. The majority of office workers (97%) have a positive attitude towards ergonomics. 75.2% of office workers in Malaysia have good ergonomic practices. Most office workers in Malaysia have poor ergonomic knowledge, positive attitudes and good ergonomic practices.