1.Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics
Efaq Ali NOMAN ; Adel Ali Al Gheethi ; Nik Norulaini Nik Ab. Rahman ; Nagao HIDEYUKI ; Balkis Binti Haji A Talip ; Radin Maya Saphira Radin Mohamed ; Mohd. Omar AB.KADIR
Malaysian Journal of Microbiology 2018;14(2):88-95
Aims: The present study aimed to recognize the microstructure of conidiophores and spores of Penicilliumspp. which were isolated from clinical wastes. Methodology and results:The isolates of Penicilliumspp. were obtained from the solid clinical wastes on V8A medium and purified by single spore method. The culture characteristics were described in five culture media included; Czapek Yeast Extract Agar(CYA); Malt Extract Agar (MEA), Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA) and Czapek-Dox Agar(CZ) while the conidiophores and spores were described using light and Scanning Electronic Microscope (SEM).Penicilliumspp. observedsome differences in their culture characteristics. Among 11 Penicilliumspecies isolated in this study and identified based on culture and microscope morphology. Five species including P. simplicissium,P. waksmanii,P. corylophilum andP. decumbensas well as one species identified as T. wortmanniiwere described in detailusing SEM. Conclusion, significance and impact of study:The study revealed that the microstructure of the fungal spores and conidiophores play an important role in the taxonomy of fungi species based on the phenotypic method.
2.Biomass quality of Scenedesmus sp. cultivated in wet market wastewater
Radin Maya Saphira Radin Mohamed ; Adel Ali Saeed Al-Gheethi ; Najeeha Mohd APANDI ; Amir Hashim Mohd Kassim ; Noor Maisara JAIS
Malaysian Journal of Microbiology 2018;14(2):120-123
Aim:This study aims to assess the wet market wastewater efficiency as Scenedesmussp. biomass production medium based on the physical and chemical characteristic as well as the biomass compositions. Methodology and results:The experiments were set up in transparent glass tank (40 L) containing 20 L of wastewater. The efficiency of different concentrations (10, 15, 20 and 25%) of wet market wastewater diluted with distilled water was compared to Bold Basal Medium (BBM). The biomass was harvested after 12 days of the incubation period by centrifugation. The quantity of biomass yielded was estimated based on the microalgae cell concentrations, while quality of biomass yield was determined by GC-MS. The nutrient contents of the raw wet market wastewater were in the range required for microalgae growth. The wastewater with 20% of dilution exhibited high efficiency for Scenedesmussp. growth in comparison to BBM (4×107vs.1 ×106cell/mL respectively). The main compounds in the microalgae biomass included cycloheptane, cyclododecanol, 1-ethenyl-acetate, 2-trifluoroacetoxypentadecane and 9-octadecenoic acid (Z)-, methyl ester.Conclusion, significance and impact of study:Scenedesmussp. biomass can be produced using the wet market wastewate