1.Comparison of nutrient-rich and limited media in the production of biosurfactant by Achromobacter xylosoxidans BP(1)5
Silvia Kurnia Sari ; Ni&rsquo ; matuzahroh ; Fatimah ; Tri Nurhariyati ; Nastiti Trikurniadewi ; Ana Mariatul Khiftiyah ; Achmad Zainal Abidin ; Khudrotun Nisa Indriyasari
Malaysian Journal of Microbiology 2022;18(2):215-221
Aims:
This study aims to produce Achromobacter biosurfactant in nutrient-rich and nutrient-limited media.
Methodology and results:
This study conducted fermentation on nutrient-rich and nutrient-limited media using a minimal salt medium (MSM). Dextrose and sodium citrate were used as sole carbon supplemented with 0.5% yeast extract for nutrient-rich media, while nutrient-limited media used molasses and rice straw hydrolysate (RSH) at variations of concentrations of 100 ppm and 200 ppm. The research was performed over 120 h and evaluated from growth response, surface tension and emulsification activity. The study revealed that the best surface tension value was when 2% (w/v) sodium citrate was used as C-source and 0.5% (w/v) yeast extract as N-source, after 72 h upon incubation at 30 °C/120 rpm having 45.45 ± 2.19 mN/m with emulsification activity 24.54 ± 3.42%. Whereas the best result of the nutrient-limited medium was obtained by RSH at a concentration of 200 ppm having 48.86 ± 5.36 mN/m.
Conclusion, significance and impact of study
The experiment showed that nutrient-limited medium from rice straw hydrolysate could compete with the nutrient-rich medium. The use of rice straw will contribute to the reduction of biosurfactant production costs and valorisation of agricultural waste.
Achromobacter denitrificans
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Surface-Active Agents