1.The in vitro and in vitro antioxidant activities of the ethyl acetate extracted from Radix Polygoni multiflori
Journal of Medicinal Materials - Hanoi 2005;10(2):59-63
Radix Polygoni multiflori has been used Vietnamese traditional medicine for a long time. This paper presented the remarkable antioxidant effects of the AcOET extract from Radix Polygoni multiflori with evidences obtained from various antioxidant tests: inhibition of lipid peroxidation in cell hemogenates, of anion superoxide production, stimulation of SOD activity using Ransod kits. The extract protected the splenocytes from H20¬2- induced injuries and protected brain cells from oxidative stress caused by an ischemia/reperfusion course. These results suggested that application of Radix Polygoni multiflori as antioxidant agent will be beneficial in prevention and treatment of diseases of the elderly
Acetates
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Antioxidants
2.Chemical constituents from ethyl acetate soluble extraction of Litsea cubeba.
Huan XIA ; Ling-Yan WANG ; Gui-Yang XIA ; Xiao-Hong WEI ; Ya-Nan WANG ; Sheng LIN
China Journal of Chinese Materia Medica 2020;45(24):5877-5883
Chemical investigation on the constituents of the ethyl acetate soluble extraction of Litsea cubeba has resulted in the isolation and structure elucidation of thirty compounds, including one sesquiterpene(1), four monoterpenes(2-5), two γ-butyrolactone derivatives(6 and 7), seven tyramine derivatives(8-14), fifteen aromatic compounds(15-29), and one pyrone derivative(30) via various chromatographic techniques and spectroscopic data analysis(MS, IR, 1 D and 2 D NMR). Compounds 1-7, 13 and 14 were obtained from the genus Litsea for the first time.
Acetates
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Litsea
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Monoterpenes
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Sesquiterpenes
3.Chemical composition and antimicrobial activities of wood vinegars from carambola, coconut shells and mango against selected plant pathogenic microorganisms
Nur Adliza Baharom ; Mohammad Hariz Abdul Rahman ; Mohammad Shahid Shahrun ; Farah Huda Sjafni Suherman ; Siti Nur Hafizah Masdar
Malaysian Journal of Microbiology 2020;16(6):438-445
Aims:
This work aimed to evaluate the antimicrobial activities of three different sources of wood vinegars obtained from
pyrolysis of carambola (Averrhoa carambola), coconut shells (Cocos nucifera) and mango (Mangifera indica) and to
identify their chemical composition.
Methodology and results:
Agar well diffusion technique was employed to assay the antifungal activity of the wood
vinegars against Fusarium oxysporum, Colletotrichum gleosporoides, and Pestalotiopsis microspora and disc diffusion
technique for antibacterial screening against Ralstonia solanacearum. The chemical compositions of these wood
vinegars were also analyzed using GC-MS by employing the headspace technique. All wood vinegars exhibited
antimicrobial activity against tested pathogens. Wood vinegar from carambola exhibited the most promising antimicrobial
effect followed by coconut shells and mango. The GC-MS analysis revealed the wood vinegars from coconut shells,
carambola, and mango are different in the chemical composition and active compounds. Major compounds identified in
coconut shells are furfural, phenol, benzofuran, acetic acid, hexanal, ethanone, and formic acid. In carambola, the main
compounds are furfural, imidazole, 3-pyridinecarboxaldehyde, benzaldehyde, phenol, benzofuran, indene, acetic acid,
indazole, naphthalene, cyclohexanecarboxylic acid, palmitamide, palmitic acid, heptadecanenitril, and sterylamide.
Meanwhile, the main chemical compounds in the pruning of mango-based vinegar consist of toluene, furfural, imidazole,
annulene, benzaldehyde, phenol, carbamic acid, acetic acid, naphthalene, heptadecanenitril, and stearylamide.
Conclusion, significance and impact of study
It is suggested that wood vinegar from carambola, coconut shells, and
mango is a promising antimicrobial agent in plant disease control, showing good potential for inhibition of selected plant
pathogenic microorganisms.
Anti-Infective Agents
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Acetates
4.The relationship between methane production metabolic flux and microorganisms in a microbial electrolytic cell coupled anaerobic digestion.
Hongzhou LIU ; Sixia YANG ; Nan WANG ; Haibo LIU ; Jianchang LI
Chinese Journal of Biotechnology 2022;38(5):1889-1902
In this study, voltage was used as a disturbance factor to investigate the relationship between microbial community and methane (CH4) production flux in a microbial electrolytic cell coupled anaerobic digestion (MEC-AD). Metabolic flux analysis (MFA) was used to explore the relationship between the CH4 metabolic flux produced and the microbes. The results showed that both methane production flux and hydrogen production flux changed significantly upon voltage disturbance, while the voltage disturbance had little effect on acetic acid production flux. The maximum CH4 production flux under 0.6 V disturbance was 0.522±0.051, which increased by 77% and 32%, respectively, compared with that of the control group under 1.0 V (0.295±0.013) and under 1.4 V (0.395±0.029). In addition, an average of 15.7%±2.9% of H2 (flux) was used to reduce CO2 to produce CH4 and acetic acid, and an average of 27.7%±6.9% of acetic acid (flux) was converted to CH4. Moreover, the abundance of Lachnospiraceae significantly affected the flux of acetic acid. The flux of CH4 production is positively correlated with the abundances of Petrimonas, Syntrophomonas, Blvii28, and Acinetobacter, and negatively correlated with the abundances of Tuzzerella and Sphaerochaeta. The species that affected the flux of H2 and CH4 were similar, mostly belonging to Bacteroides, Clostridium, Pseudomonas and Firmicutes. Furthermore, the interspecies interaction is also an important factor affecting the MEC-AD methanogenesis flux.
Acetates
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Anaerobiosis
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Bioreactors
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Electrolysis
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Methane
5.Association of cytoplasmic phospholipase A2 gene polymorphism with bronchial asthma and response to montelukast in children.
Qing GUO ; Zhao-Bo SHEN ; Xiao-Min SUN ; Dan CHEN ; Ping KANG
Chinese Journal of Contemporary Pediatrics 2019;21(2):155-160
OBJECTIVE:
To study the association of cytoplasmic phospholipase A2 (PLA2G4) rs932476 polymorphism with the development of bronchial asthma and the response to montelukast, a leukotriene receptor antagonist, in children.
METHODS:
A total of 128 children with bronchial asthma were enrolled as case group, and 100 healthy children were enrolled as control group. The genotype and allele frequencies of PLA2G4 rs932476 were compared between the two groups. The children in the case group were administered with montelukast except routine treatment for 2 months, and the changes in serum levels of leukotriene B4 (LTB4), interleukin-4 (IL-4), immunoglobulin E (IgE), and interferon gamma (IFN-γ), pulmonary function and fractional exhaled nitric oxide (FeNO) after treatment were observed.
RESULTS:
There were no significant differences in the genotype and allele frequencies of PLA2G4 rs932476 between the case and control groups, as well as between the groups with different severities of asthma (P>0.05). After treatment, the children with AA genotype had a significantly higher overall response rate than those with GG genotype. After treatment, the case group had significant reductions in the serum levels of IgE and IL-4 and a significant increase in the level of IFN-γ (P<0.05). After treatment, the children with GG genotype had a higher serum level of IL-4 and a lower level of IFN-γ than those with AA genotype. After treatment, the case group had significant increases in pulmonary function parameters, and the children with AA genotype had significantly higher parameters than those with GG genotype. The case group had a significant reduction in the level of FeNO, and the children with AA genotype had a significantly lower level than those with GG genotype after treatment. The case group had a significantly higher serum level of LTB4 than the control group before treatment (P<0.05). After treatment the case group had a significant reduction in the serum level of LTB4 (P<0.05). The children with GG genotype had a significantly higher level of LTB4 than those with AA genotype after treatment (P<0.05).
CONCLUSIONS
PLA2G4 rs932476 polymorphism is not associated with the susceptibility and severity of bronchial asthma in children, but it may has certain influence on children's response to the leukotriene receptor antagonist montelukast, possibly by affecting the level of LTB4.
Acetates
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Asthma
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Child
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Humans
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Leukotriene Antagonists
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Quinolines
6.A Case of Pressure Urticaria Treated with a Leukotriene Inhibitor.
Young Wook LEE ; Min Hee KANG ; Na Reu SEUNG ; Eun Ju PARK ; Chul Woo KIM ; Kwang Ho KIM ; Kwang Joong KIM
Korean Journal of Dermatology 2009;47(5):588-591
Delayed pressure urticria is a type of physical urticaria, characterized by the appearance of painful swelling for a few hours after physical stimulus. A 27-year-old woman suffered from pressure urticaria for eight months. The patient was unresponsive to conventional antihistamine, glucocorticoid, cyclosporine and azathioprine. Since the patient was treated with 10 mg/day montelukast and 1 mg/day ketotifen for 10 months, erythema and wheals have not developed. We report a case of pressure urticaria treated with a leukotriene inhibitor.
Acetates
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Adult
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Azathioprine
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Cyclosporine
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Erythema
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Female
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Humans
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Ketotifen
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Quinolines
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Urticaria
7.Chemical study on ethyl acetate portion of Ehretia thyrsiflora, boraginaceae species of Kudingcha.
Li LI ; Li-jia XU ; Zhen-dan HE ; Qin-qiong YANG ; Yong PENG ; Pei-gen XIAO
China Journal of Chinese Materia Medica 2008;33(17):2121-2123
OBJECTIVETo investigate the chemical constituents of Ehretia thyrsiflora.
METHODCompounds were isolated by using silica gel, Sephadex LH-20 and RP-C18 chromatography; their structures were elucidated by means of spectral data analysis.
RESULTSeven compounds were isolated and identified as methyl rosmarinate (1), caffeic acid (2), quercetin (3), kampferol (4), kaempferol 3-O-alpha-D-arabinoside (5), quercetin 3-O-alpha-D-arabinoside (6), and p-hydroxy benzoic acid (7).
CONCLUSIONAll these compounds were isolated from E. thyrsiflora for the first time. Compounds 2-7 were isolated from genus Ehretia for the first time.
Acetates ; analysis ; Boraginaceae ; chemistry ; Drugs, Chinese Herbal ; chemistry
9.Remarkable rate acceleration of SmI3-mediated iodination of acetates of Baylis-Hillman adducts in ionic liquid: facile synthesis of (Z)-allyl iodides.
Yun-kui LIU ; Hui ZHENG ; Dan-qian XU ; Zhen-yuan XU ; Yong-min ZHANG
Journal of Zhejiang University. Science. B 2006;7(3):193-197
Stereoselective transformation of Baylis-Hillman acetates 1 into corresponding (Z)-allyl iodides 2 has been achieved by treatment of 1 with samarium triiodide in THF. Remarkable rate acceleration of samarium triiodide-mediated iodination of 1 was found when ionic liquid 1-n-butyl-3-methyl-imidazolium tetrafluroborate ([bmim]BF(4)) was used as reaction media in stead of THF. This novel approach proceeds readily at 50 degrees C within a few minutes to afford (Z)-allyl iodides 2 in excellent yields. A mechanism involving stereoselective iodination of the acetates of Baylis-Hillman adducts by samarium triiodide is described, in which a six-membered ring transition state played a key role in the stereoselective formation of 2.
Acetates
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chemistry
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Iodides
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chemical synthesis
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Ions
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Kinetics
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Samarium
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chemistry
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Solutions
10.Chemical study on ethyl acetate soluble portion of Kadsura oblongifolia.
Haitao LIU ; Lijia XU ; Yong PENG ; Rongtao LI ; Peigen XIAO
China Journal of Chinese Materia Medica 2009;34(7):864-866
OBJECTIVETo study the chemical constituents in the stems of Kadsura oblongifolia.
METHODThe chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.
RESULTSeven compounds named kaempferol-3-O-alpha-L-arabinofuranoside (1), kaempferol-3-O-alpha-D-arabinopyranoside (2), quercetin-3-O-alpha-D-arabinopyranoside (3), quercetin-3-O-alpha- L-arabinofuranoside (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin (6), kaempferol (7) were isolated and identified.
CONCLUSIONCompounds 1-7 were obtained from this plant for the first time. Compounds 1-4 were isolated from Schisandraceae family for the first time.
Acetates ; chemistry ; Kadsura ; chemistry ; Plant Stems ; chemistry ; Solubility