1.Study on the diuretic and electrolites excuted effecs of bach hac on experimental animals
Journal of Medical Research 2003;26(6):22-26
Diuretic effect of Bach hac fluid extract was investigated on white rat. Results showed that Bach hac (Acaramthus nasutin – Acanthaceae) in the dose of 4g/kg – 6g/kg body weight has increased the elimination of Na+, K+, Cl- and Ca++ ions through kidney, the effect was most obvious in the 2nd and 4th hours after administration under any doses.
Animal Experimentation
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diuretics
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Acanthaceae
2.Study on chemical components of Barleria lupulina Lindl., Acanthaceae
Pharmaceutical Journal 1998;272(12):13-14
Materials: leaves and stem of Kimvang (Barleria lupulina Lindl., Acanthaceae) in Mytho city, Cantho province. Methods: extraction by fractional extraction; qualitative analyis by test tube, thin layer chromatography (TLC) and spectrometry. The results: Kimvang contained 12 elements including silicone, magnesium, calcium, strontium, iron, manganum, titanum, copper, natrium, lead and phosphorum and saponine, triterpenoid, flavonoid, phytosterol, tamine pyrocatechic, carotenoid, reduced sugar and fat. The leaves of Kimvang contained compound F (derivative of flavon).
chemistry
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Acanthaceae
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Plants, Medicinal
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Medicine, Traditional
3.Acanthus ilicifolius L. Treatment for Oral Candidiasis with Immunosuppressive Conditions Subjected to p38 MAPK Enhancement
Dwi Andriani ; Agni Febrina Pargaputri ; Kristanti Parisihni ; Syamsulina Revianti
Archives of Orofacial Sciences 2021;16(SUPP 1):17-24
ABSTRACT
Methanolic extract from the leaves of Acanthus ilicifolius L. (A. ilicifolius L.) is a potent inhibitor of
Candida albicans (C. albicans) growth and anti-inflammatory. C. albicans causes oral candidiasis in
immunosuppressive condition. Mitogen-activated protein kinase (MAPK) signalling via p38 appears
to discriminate between yeast and hyphal cells of C. albicans. Activation of p38 MAPK by hyphae
results in the upregulation of proinflammatory cytokines. The p38 MAPK activation is known to
impair corticosteroid action. The research was conducted to investigate the effect of methanolic
extract A. ilicifolius L. treatment of oral candidiasis with the immunosuppressive condition through
enhancement of p38 MAPK expression in the epithelial cells. Immunosuppressed conditions
were obtained when 16 healthy male Rattus norvergicus (Wistar) was given oral administration
of dexamethasone and tetracycline for 14 days and induced with C. albicans (ATCC-10231)
1 McFarland. The subjects were divided into four groups (n = 4/group): immunosuppression
(IS), immunosuppression with oral candidiasis without treatment (ISC), immunosuppression
with oral candidiasis and nystatin treatment (ISC+N), and immunosuppression with oral
candidiasis and A. ilicifolius L. treatment (ISC+AI), and were treated for 14 days. Later, the rats
were euthanised, and their tongue were biopsied. The p38 MAPK expression was subjected to
immunohistochemical examination, observed under a microscope (400× magnification) and
statistically analysed (one-way ANOVA, LSD-test, p < 0.05). The p38 MAPK expression of
ISC+AI (36.05 ± 1.54) was higher than IS (26 ± 2.32), ISC (26.4 ± 3.71), IS+N (34.2 ± 0.99).
Significant differences existed between ISC+AI and ISC+N to IS and ISC (p < 0.05). No significant
differences were present between IS and ISC; ISC+AI and ISC+N (p > 0.05). Therefore, this treatment
could enhance p38 MAPK expression in oral candidiasis with the immunosuppressed condition.
Acanthaceae
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Candidiasis, Oral
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Immunosuppression Therapy
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p38 Mitogen-Activated Protein Kinases
4.Studies on structure characteristic of polysaccharide P1A from Dicliptera chinensis.
Yu-yuan HE ; Lu-wei XIE ; Guan-feng DU ; Jiang-cheng ZHAN ; Yong-qin YIN ; Zhi-bin SHEN
China Journal of Chinese Materia Medica 2015;40(8):1489-1492
The chemical structures of P1 A was identified by complete acid hydrolysis, partial acid hydrolysis, periodate oxidation-Smith degradation, methylation analysis, IR and NMR. The results showed that P1 A had a backbone consisting rhamnose, mannose, glucose and galactose. The side chain possessed arabinose and xylose. 1-->, 1-->6 and non-reducing terminal linkages existed in polysaccharide P1A, but there are doubling amount of 1-->2 and 1-->4 linkages. Oxidable linkage of P1 A accounted for 45%, and inoxidable linkage of P1A accounted for 55%. Mannose, glucose and galactose were mainly linked by 1-->2 linkage. Rhamnose, arabinose and xylose were mainly linked by 1-->2 and 1-->4 linkages. PlA contained beta-Glc(1,6)-,beta-Gal(1,3)-,beta-Man(1,4)-beta-Rha,-Glc(1,4)-, Glc(1)-,-Gal(1,4)- and Man(1)-.
Acanthaceae
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chemistry
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Drugs, Chinese Herbal
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chemistry
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Molecular Weight
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Polysaccharides
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chemistry
5.Alternaria yunnanensis sp. nov., a New Alternaria Species Causing Foliage Spot of Rubber Tree in China
Zhi Ying CAI ; Yi Xian LIU ; Yu Ping SHI ; Li Ming DAI ; Lan Lan LI ; Hong Jun MU ; Mei Lin LV ; Xiao Yong LIU
Mycobiology 2019;47(1):66-75
A new species of Alternaria causing leaf spots on the rubber tree (Hevea brasiliensis) in Yunnan, China, was isolated, examined, and illustrated. Morphologically, it belongs to the section Porri of Alternaria, which produces relatively large conidia and a simple or branched, filamentous long beak. It is, however, characterized by conidiophores gradually enlarging near the apex into a clavate conidiogenous cell and long ellipsoid to obclavate, smooth-walled conidia with a long filamentous beak. Molecular phylogenetic analyses based on ITS rDNA, GAPDH, and TEF1-alpha sequences demonstrate that the phytopathogen falls in the clade of the section Porri, being most closely related to A. sidae, A. sennae, A. deseriticola, A. cyamopsidis, A. rostellata, A. nitrimali, A. crassa, and A. thunbergiae.
Acanthaceae
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Accidental Falls
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Alternaria
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Animals
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Ascomycota
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Beak
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China
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Classification
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DNA, Ribosomal
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Hevea
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Rubber
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Spores, Fungal
6.Study on chemical constituents of mangrove Acanthus ilcifolius.
Chang-Hong HUO ; Bin WANG ; Hong LIANG ; Yu-Ying ZHAO ; Wen-Han LIN
China Journal of Chinese Materia Medica 2006;31(24):2052-2054
OBJECTIVETo study the chemical constituents of Acanthus ilicifolius.
METHODChromatographic methods were used to isolate compounds from A. ilicifolius, and chemical and spectroscopic methods were used to elucidate the structures of the isolated compounds.
RESULTSeven compounds, betaine (1), phenylethyl-O-beta-D-glucopyranosyl- (1-->2) -beta-D-glucopyranoside (2), phenylethyl-O-beta-D-glucopyranoside (3), acteoside (4), isoacteoside (5), benzyl-O-beta-D-glucopyranoside (6) and vanillic acid (7) were obtained.
CONCLUSION1, 3, 6 and 7 were obtained from the genus for the first time.
Acanthaceae ; chemistry ; Betaine ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Vanillic Acid ; chemistry ; isolation & purification
7.Studies on the chemical constituents in herbs of ethanolic extract from herbs of Dicliptera chinensis.
Yu-tao GAO ; Xiu-wei YANG ; Tie-min AI
China Journal of Chinese Materia Medica 2006;31(12):985-987
OBJECTIVETo study the chemical constituents of ethanolic extract from herbs of Dicliptera chinensis.
METHODThe compounds were separated by silica gel column chromatography, preparation TLC and reverse phase HPLC, their structures were identified by the spectroscopic methods of UV, NMR and MS.
RESULTSeven compounds were isolated from ethyl acetate extract. Their structure were identified as octasulphur (1), secoisolariciresinol dimethyl ether diacetate (2), 5-methoxy-4, 4'-di-O-methyl secolariciresinol (3), chinensinaphthol methyl ester (4), loliolide (5), beta-sitosterol 3-O-beta-D-glucopyranoside (6) and stigmasterol 3-O-beta-D-glucopyranoside (7).
CONCLUSIONAll the compounds except 6 were obtained from the plants of Dicliptera for the first time.
Acanthaceae ; chemistry ; Benzofurans ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Sitosterols ; chemistry ; isolation & purification
8.Bioinformation analysis of chorismate synthase in Baphicacantus cusia and other 78 species of plants.
Jian YU ; Qi YE ; Shu-Ju NING ; Qing LI ; He-Xin TAN ; Jing-Xian FENG ; Rui-Bing CHEN ; Xiao-Li MA ; Pei-Min GONG ; Xuan-Xuan ZHAO ; Lei ZHANG ; Dao-Zhi WEI
China Journal of Chinese Materia Medica 2018;43(4):721-730
Chorismate synthase(CS, EC:4.2.3.5) catalyses 5-enolpyruvy-shikimate-3-phosphate to form chorismate, which is the essential enzyme for chorismate biosynthesis in organisms. The amino acid sequences of CS from 79 species of higher plants were reported in GenBank at present. 125 amino acid sequences of CS from Baphicacanthus cusia and other 78 species of plants were predicted and analyzed by using various bioinformatics software, including the composition of amino acid sequences, signal peptide, leader peptide, hydrophobic/hydrophilic, transmembrane structure, coiled-coil domain, protein secondary structure, tertiary structure and functional domains. The phylogenetic tree of CS protein family was constructed and divided into eight groups by phylogenetic analysis. The homology comparison indicated that B. cusia shared a high homology with several plants such as Sesamum indicum, Nicotiana tabacum, Solanum tuberosum and so on. The open reading frame(ORF) of all samples is about 1 300 bp, the molecular weight is about 50 kDa, the isoelectric point(pI) is 5.0-8.0 which illustrated that CS protein is slightly basic. The ORF of CS we cloned in B. cusia is 1 326 bp, the amino acid residues are 442, the molecular weight is 47 kDa and pI is 8.11. The CS in B.cusia showed obvious hydrophobicity area and hydrophilicity area, no signal peptide, and may exists transmembrane structure areas. The main secondary structures of CS protein are random coil and Alpha helix, also contain three main structural domains which are an active structural domain, a PLN02754 conserved domain and a FMN binding site. The acquired information in this study would provide certain scientific basis for further study on structure-activity relationship and structure modification of CS in plants in the future.
Acanthaceae
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enzymology
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Amino Acid Sequence
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Computational Biology
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Phosphorus-Oxygen Lyases
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chemistry
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Phylogeny
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Plant Proteins
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chemistry
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Protein Structure, Secondary
9.Experimental Life History and Biological Characteristics of Fasciola gigantica (Digenea: Fasciolidae).
Anawat PHALEE ; Chalobol WONGSAWAD ; Amnat ROJANAPAIBUL ; Jong Yil CHAI
The Korean Journal of Parasitology 2015;53(1):59-64
This study was conducted to investigate the life history, morphology, and maturation of larval stages and adult worms of Fasciola gigantica in experimental mice. Lymnaea auricularia rubiginosa was used as the intermediate host, and Oryza sativa was used for encystment of the metacercariae, while Mus musculus was used as the definitive host for maturation study. Fresh eggs from the gall bladder of water buffaloes fully developed into embryonated ones and hatched out at days 11-12 after incubation at about 29masculineC. Free-swimming miracidia rapidly penetrated into the snail host, and gradually developed into the next larval stages; sporocyst, redia, and daughter redia with cercariae. Fully-developed cercariae were separated from the redia and shed from the snails on day 39 post-infection (PI). Free-swimming cercariae were immediately allowed to adhere to rice plants, and capsules were constructed to protect metacercariae on rice plants. Juvenile worms were detected in intestines of mice at days 3 and 6 PI, but they were found in the bile duct from day 9 PI. Juvenile and adult flukes were recovered from 16 mice experimentally infected with metacercariae, with the average recovery rate of 35.8%. Sexually mature adult flukes were recovered from day 42 PI. It could be confirmed that experimentally encysted metacercariae could infect and develop to maturity in the experimental host. The present study reports for the first time the complete life history of F. gigantica by an experimental study in Thailand. The obtained information can be used as a guide for prevention, elimination, and treatment of F. gigantica at environment and in other hosts.
Acanthaceae/parasitology
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Animals
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Buffaloes/parasitology
;
Fasciola/*anatomy & histology/*physiology
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Gallbladder/parasitology
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Larva/anatomy & histology/physiology
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*Life Cycle Stages
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Mice
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Microscopy
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Oryza sativa/parasitology
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Time Factors
10.Experimental Life History and Biological Characteristics of Fasciola gigantica (Digenea: Fasciolidae).
Anawat PHALEE ; Chalobol WONGSAWAD ; Amnat ROJANAPAIBUL ; Jong Yil CHAI
The Korean Journal of Parasitology 2015;53(1):59-64
This study was conducted to investigate the life history, morphology, and maturation of larval stages and adult worms of Fasciola gigantica in experimental mice. Lymnaea auricularia rubiginosa was used as the intermediate host, and Oryza sativa was used for encystment of the metacercariae, while Mus musculus was used as the definitive host for maturation study. Fresh eggs from the gall bladder of water buffaloes fully developed into embryonated ones and hatched out at days 11-12 after incubation at about 29masculineC. Free-swimming miracidia rapidly penetrated into the snail host, and gradually developed into the next larval stages; sporocyst, redia, and daughter redia with cercariae. Fully-developed cercariae were separated from the redia and shed from the snails on day 39 post-infection (PI). Free-swimming cercariae were immediately allowed to adhere to rice plants, and capsules were constructed to protect metacercariae on rice plants. Juvenile worms were detected in intestines of mice at days 3 and 6 PI, but they were found in the bile duct from day 9 PI. Juvenile and adult flukes were recovered from 16 mice experimentally infected with metacercariae, with the average recovery rate of 35.8%. Sexually mature adult flukes were recovered from day 42 PI. It could be confirmed that experimentally encysted metacercariae could infect and develop to maturity in the experimental host. The present study reports for the first time the complete life history of F. gigantica by an experimental study in Thailand. The obtained information can be used as a guide for prevention, elimination, and treatment of F. gigantica at environment and in other hosts.
Acanthaceae/parasitology
;
Animals
;
Buffaloes/parasitology
;
Fasciola/*anatomy & histology/*physiology
;
Gallbladder/parasitology
;
Larva/anatomy & histology/physiology
;
*Life Cycle Stages
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Mice
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Microscopy
;
Oryza sativa/parasitology
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Time Factors