2.A Child with Septic Arthritis of Hip: A Rarely Encountered Cause
A R Abdul Halim ; Y Norhamdan ; R Ramliza
The Medical Journal of Malaysia 2011;66(2):154-155
We report a rare case of septic arthritis in a healthy child caused by Salmonella enteritidis. No predisposing factor was detected. Salmonella enteritidis was isolated from the infected joint tissue obtained following surgical drainage. Based on the culture and sensitivity report, he was treated with a 6-week course of antibiotic. He improved dramatically without any detrimental sequelae at end of one year.
3.Tyrisonase inhibition and melanin reduction of human melanocytes (HEMn-MP) using Anacardium occidentale L extract.
Abdul Gaffar R ; Abdul Majid FA ; Sarmidi MR
The Medical Journal of Malaysia 2008;63 Suppl A():100-101
Cashew (Anacardium occindentale L) leaves extract (CLE) has potential as tyrosinase inhibitor that can be used for therapeutic in pigmentation problem. This study investigates the real potential of CLE to inhibit tyrosinase and melanin reduction using human epidermal melanocytes. The extracts were exposed to the human melanocytes for more than 24 hours. The CLE extract exhibited potential as tyrosinase inhibitor, reduced melanin and high in antioxidant activity relative to commercial extract of Emblica sp.
4.An in vivo study of a locally-manufactured hydroxyapatite-based material as bone replacement material.
N H Abdul Razak ; K A Al-Salihi ; A R Samsudin
The Medical journal of Malaysia 2004;59 Suppl F():119-20
Defects were created in the mandible of a rabbit model whereby the right side was implanted with hydroxyapatite (HA) while the left side was left empty to act as control. Both the implant and control sites were evaluated clinically and histologically at 4,12,20,22 weeks. Decalcified sections were studied under confocal laser scanning microscope. No reactive cells were evident microscopically in all sections. There was bone ingrowth as early as 4 weeks when viewed by the topographic method. Enhancement of osteoconduction was evident by the presence of abundant capillaries, perivascular tissue and osteoprogenitor cells of the host. At 22 weeks, the implanted defect showed mature bone formation filling almost the whole field. This study demonstrated that the dense HA exhibits excellent biocompatibility as noted by the complete absence of reactive cells. It also promotes osteoconduction.
Materials
;
Skeletal bone
;
week
;
Durapatite
;
in vivo study
5.Presence of faecal coliforms and selected heavy metals in ice cubes from food outlets in Taman Universiti, Johor Bahru, Malaysia
Mahat, N.A., Meor Ahmad, Z., Abdul Wahab, R.
Tropical Biomedicine 2015;32(3):471-477
Consumption of iced beverages is common in Malaysia although specific research
focusing on its safety parameters such as presence of faecal coliforms and heavy metal
elements remains scarce. A study conducted in Kelantan indicated that faecal coliforms
were detected in the majority of the ice cube samples analyzed, largely attributable to
improper handling. Hence, it was found pertinent to conduct similar study in other parts of the
country such as Johor Bahru if the similar pattern prevailed. Therefore, this present crosssectional
study which randomly sampled ice cubes from 30 permanent food outlets in Taman
Universiti, Johor Bahru for detecting contamination by faecal coliforms and selected heavy
metal elements (lead, copper, manganese and zinc) acquires significance. Faecal coliforms
were detected in 11 (36.67%) of the samples, ranging between 1 CFU/100 mL to > 50 CFU/100
mL; two of the samples were grossly contaminated (>50 CFU/100 mL). Interestingly, while
positive detection of lead was observed in 29 of the 30 ice cube samples (mean: 0.511±0.105
ppm; range: 0.489-0.674 ppm), copper, manganese and zinc were not detected. In addition,
analysis on commercially bottled mineral water as well as in tap water samples did not detect
such contaminations. Therefore, it appears that (1) contamination of faecal coliforms in ice
cubes in food outlets in Malaysia may not be sporadic in pattern but rather prevalent and (2)
the source of water used for manufacturing the ice cubes that contained significant amount
of lead would suggest that (3) it was neither originated from the treated tap water supply nor
bottled mineral water or (4) perhaps contaminated during manufacturing process. Further
studies exploring the source of water used for manufacturing these ice cubes as well as the
handling process among food operators deserve consideration.
6.Identification and characterization of Raoutella ornithilolytica and determination of its herbicide 2,2-dichloropropionate (2,2-DCP) degradation potential
Rajabpour Niknam M ; Huyop F ; Abdul Wahab R
Malaysian Journal of Microbiology 2014;10(4):249-254
Aims: The goal of the study is to isolate new bacteria species which are capable to utilizing 2,2-dichlropropionic acid (2-
2-DCP) as a sole carbon source from the wastewater sample that was taken from Tioman Island off the coast of
Malaysia.
Methodology and results: Genomic DNA from unknown bacterial strain (MR1) was extracted and PCR amplification
was carried out using universal primers, Fd1 (5’-AGA GTT TGA TCC TGGCTC AG-3’) and rP1 (5’-ACG GTC ATA CCT
TGT TAC GAC TT-3’) prior to sequencing. The BLASTn and phylogenetic analysis of the 16S rRNA of the MR1 gene
found it shares a 95% homology to an aerobic bacillus Raoutella ornithilolytica. The bacteria which is usually found in an
aquatic and hospital environment was found to possess a distinctive feature of being able to utilize 2,2-DCP as sole
carbon source. The bacteria has cell doubling time of 23.11 h and maximum release of chloride ion at 0.257 mmol/L in
20 mM 2,2-DCP. Based on morphological and partial biochemical characteristics, the strain was found to be non-motile,
Gram negative bacteria with red colonies that gave a positive catalase reaction.
Conclusion, significance and impact of study: A better understanding of newly isolated microorganisms from the
environment which can potentially be used as bioremediation tools in environmental management. This is the first
reported case of Raoutella sp. that has the unique ability to degrade halogenated compound. Hence, the dehalogenation
properties and capabilities of the bacteria deserve to be assessed further.
7.Correlation of Rheumatoid Factor Serotypes and Computed Tomography Findings in Rheumatoid Arthritis Related Interstitial Lung Disease
SAKTHISWARY R ; RADHIKA S ; SYAHRUL SAZLIYANA S ; ABDUL WAHAB A
Medicine and Health 2019;14(2):203-211
The most common extra-articular manifestation of rheumatoid arthritis (RA) is interstitial lung disease (ILD). RA related ILD (RA-ILD) is associated with significant morbidity and mortality. The main objective of this study was to determine the correlation between the rheumatoid factor (RF) serotypes and the severity of RA-ILD based on computed tomography (CT) findings. We recruited a total of 100 RA patients who were tested for IgA RF, IgG RF and IgM RF and had high resolution CT chest performed. Seventy-two patients had ILD changes on HRCT of the chest and were included in this study. We found that the the CT scores for ground glass showed significant positive correlation with disease duration and IgA RF levels whereas the fibrosis scores had significant relationship with multiple clinical covariates i.e age, disease duration, IgA RF levels, IgG RF levels and anti-CCP levels. On multivariate analysis, only IgA levels remained significantly (p<0.05, standardized beta coefficient = 0.604) associated with the ground glass scores. Regarding the fibrosis scores, IgA RF levels and age were independent predictors based on multivariate analysis after adjusting for confounders, with p scores of <0.05 and 0.02, respectively. In conclusion, the IgA RF was the only serotype which was independently associated with the severity of RA-ILD.
8.Assessment of biodegradability of PVC containing cellulose by white rot fungus
Fakhrul, H., Fazal A ; Farooq R ; Sohaib R ; Abdul G ; Muhammad S
Malaysian Journal of Microbiology 2014;10(2):119-125
Aims: Polyvinyl chloride (PVC) is the most widely used and environmentally damaging plastic. Processing, production
and disposal of PVC cause release of toxic chlorine based compounds into environment. The objective of the present
study was to assess the biodegradability of cellulose blended PVC by white rot fungi i.e. Phanerochaete chrysosporium.
Methodology and results: Biodegradability of the strain for the polymer was tested on plate assay, sturm test, soil
burial and shake flask experiments. The biodegradability of the polymer was determined by visual changes, plate assay
and carbon dioxide production. Morphological changes in the polymer such as pits, extensive spotting, clear surface
erosion, fungal attachment, roughening and deterioration of some parts were observed using scanning electron
microscopy. Chemical changes like appearance and shortening of peaks using fourier transform infrared spectroscopy
also confirmed the biodegradability of the polymer.
Conclusion, significance and impact of study: The present study confirmed that mixing of small amount of cellulose
increases the hydrophilicity of the polymer and lead to its microbial degradation and Phanerochaete chrysosporium has
great potential for the treatment of solid waste containing plastics.
9.Fine needle aspiration cytology of neuroendocrine carcinoma of the breast--a case report and review of literature.
Abdul A R Kadir ; Krishnan R Iyengar ; Suat Cheng Peh ; Cheng Har Yip
The Malaysian journal of pathology 2008;30(1):57-61
Neuroendocrine carcinomas of the breast are uncommon tumors known to occur in the elderly. While focal neuroendocrine differentiation may be noted in many ductal and lobular carcinomas, the term neuroendocrine carcinoma is to be applied when more than 50% of the tumor shows such differentiation. This case report details the cytological features of a neuroendocrine carcinoma that was encountered in our hospital. The fine needle aspiration (FNA) smears showed discohesive polygonal cells with abundant cytoplasm, many of which contained eosinophilic granules located at one pole. Histology of the mastectomy and axillary lymph nodes specimen from this patient showed features of neuroendocrine carcinoma--solid type, with metastasis, confirmed with immunohistochemistry. The patient is disease free seven months after surgery. This case highlights the need to closely observe cytological details to identify this rare tumor that may otherwise appear to be invasive duct carcinoma--not otherwise specified on FNA. The implications of diagnosing neuroendocrine differentiation for prognosis and management are also discussed.
Carcinoma, Neuroendocrine
;
Fine needle biopsy, NOS
;
differentiation
;
Case Report
;
Breast
10.Thalassaemia: A Study on The Perception of Patients and Family Members
J Abdul Wahab ; M Naznin ; M Z Nora ; A R Suzanah ; M Zulaiha ; A R Aidil Faszrul ; W S Kamaruzaman
The Medical Journal of Malaysia 2011;66(4):326-334
Marked improvement in the management of thalassaemia
has not been matched by progress in psychosocial
rehabilitation as thalassaemia continues to pose challenges
to patients and their family members. Few studies have been
carried out in Malaysia to look at such issues. This study is therefore to explore the concerns, beliefs and feelings about thalassaemia. It was conducted in the year 2009 over 7 months on “focus groups”, in patients aged 8-22 years and
parents attending Paediatric Clinic of Tengku Ampuan Afzan
Hospital, Kuantan, Pahang. Results showed that concerns
and adverse impact were related to lower grades in
education, poor self-image, less chance of employment,
marriage, financial burden and social integration.
Compliance to subcutaneous iron chelator was poor. There
were various concerns related to blood transfusion therapy.
It is evident that thalassaemia greatly affects the
psychosocial dimensions and a more structured long term
psychosocial support is needed to improve quality of life of patients.