The effects of resistance training (RT) on serum adipocytokine levels were investigated in rats. Male rats were allocated either to a RT group or a control (C) group. The RT group performed RT by climbing 1-m high cages for access to water. The epididymal, retroperitoneal, and inguinal fat pad weights of the RT were significantly lesser than those of the C group. A significantly lower serum insulin concentration was observed in the RT group than in the C group ; no significant group difference was observed in the plasma glucose concentration. The serum leptin level was significantly lower in the RT group than in the C group. On the other hand, no significant difference existed in the TNF-α, adiponectin, and resistin levels between the two groups. The results indicate that the RT improved the whole-body insulin sensitivity and induced differential effects on the serum adipocytokine levels in rats.