Objective To investigate the clinical significance of the detection of the levels of serum uric acid (UA) and serum eytokine tunor necrosis factor-oα (TNF-α),soluble intercellular adhesion molecule-1 (sICAM-1),interleukin (IL)-6 and IL-8 in patients with chronic obstructive pulmonary disease (COPD).Methods UA of 40 healthy people (control group) and 158 COPD patients (COPD group) in acute exacerbation and remission stage was detected respectively by automatic biochemical analyzer,and the levels of serum TNF-α,sICAM-1,IL-6 and IL-8 were detected by ELISA.The percentage of first second forced expiratory volume in expected value (FEV1％) of COPD patients in acute exacerbation and remission stage were detected.Results TNF-α,sICAM-1,IL-6,IL-8 and UA of COPD group in acute exacerbation were significantly higher than those of control group (P＜ 0.01 ),and FEV1％ was significantly lower than that of control group (P＜ 0.01 ).TNF-α,sICAM-1 and UA of COPD group in remission stage were significantly higher than those of control group (P ＜0.01 or ＜0.05),IL-6 and IL-8 had no statistical significance compared with those of control group (P ＞ 0.05),and FEV1％ was significantly lower than that of control group (P ＜ 0.01 ).Correlation analysis showed that TNF- α,sICAM-1,IL-6,IL-8,UA of COPD patients in acute exacerbation were negatively conrelated with FEV1％ (r =-0.681,-0.579,-0.545,-0.652,-0.357,respectively,P ＜ 0.01 or ＜ 0.05 ).In remission stage,TNF- α,sICAM-1,IL-6 and IL-8 of COPD patients were negatively correlated with FEV1％ (r =-0.623,-0.335,-0.349,-0.623,P＜0.01 or ＜0.05),and UA had no significant correlation with FEV1％ (r =-0.092,P ＞0.05).Conclusions The serum UA and cytokines TNF-α,sICAM-1,IL-6,IL-8 are involved in the progression of COPD and correlated with lung function.They can be used as the judge indexes of COPD.

WJ multipurpose guided puncture outfit in interventional ultrasound was developed.Guided puncture with this outfit was performed on 203 cases of various diseases.All 274 punctures were saccessful The study shows hat this guided puncture outfit can be set on various linear probes,fit-ted into puncture,biolpsy or catheter needles.It can actively adjust the puncture angle,precisely forecast,fix and calibrate the puncture angle and depth,perlorm guided puncture in real time etc.It is notnecessary to set line and tabulate the puncture angle.The outfit is easy and rapid to handle and also is reliable with high success rate

Objective:To probe the mechanism of inhibition in gap junction-mediated intercellular communication (GJIC) of rabbit lens epithelia cells(RLECs) irradiated by UVA. Methods:RLECs of 2～3 generation were divided into normal control group and ultraviolet irradiation group (The UV intensity was 0.2 mW/cm2 under a UV lamp with fixed position 50 cm, RLECs were exposed to ultraviolet lamp at different times: 5,10,15 minutes). Western blot techniques were employed to detect the protein levels of Cx43 irradiated by UVA. By indirect immunofluorescence histochemistry we analyzed the localization of Cx43 in the RLECs. Results:In UVA irradiation group and normal control group,there was expression of Cx43. With the increasing intensity,the expression of Cx43 was decreasing. The immunnostaining of Cx43 in RLECs revealed that the internalization of Cx43 from membrance to plasma and nuclear was found in UVA irradiated RLECs. Conclusion: Cell molecule level study proved UVA irradiation can reduce GJIC of RLECs,which wis associated with perturbations in localization and number of Cx43 among RLECs.

Objective:To study the deficiency ofshort stature homeobox containing (SHOX)gene deficiency ofshort stature and the relationship between genotypes and its corresponding phenotypes. Methods:Microsatellite analysis and direct sequencing were selected to analyze the deletions and mutations of SHOX gene in 203 cases of short stature;in addition ,anthropometric measures were assessed and radiographs of the left forearms and wrists in patients were collected to observe their phenotypes. Results:one mutation and eleven deletions were found;Detailed examination revealed certain dysmorphics,such as short forearm and lower leg,et al. Conclusion:In our study,the prevalence of SHOX deficiency in patients with short stature was 5.9% ,and certain correlations had been found between genotypes and corresponding phenotypes。

GATA4 gene mutations are common in children with congenital heart disease.More than 120 mutations have been found in ventricular septal defect,atrial septal defects and the tetralogy of fallot.But the relationship between genotype and clinical phenotype has not yet been elucidated.This article summarizes the published germline and somatic mutations of GATA4 gene in human congenital heart disease,and provides insight into the phenotypic spectrum of GATA4 gene mutation.

Objective To detect the expression of insulin-like growth factor Ⅱ mRNA-binding protein 3 (IMP3) and p16 in cervical carcinoma and investigate their clinical significance.Methods Immunohistochemical Envision method was used to detect the expression of IMP3 and p16 proteins in 67 cases of carcinoma of the uterine cervix,58 cases of cervical intraepithelial neoplasia (CIN) and 15 cases of normal cervical epithelium.Results IMP3 was mainly dedected in the cytoplasm,whose positive expression rate was 85.07％ (57/67) in cervical carcinoma,12.07％ (7/58) in CIN (x2 =66.32,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =44.38,P ＜ 0.05).No significance variations of the expression of IMP3 were observed in cervical adenocarcinoma and squamous cell carcinoma (x2 =0.35,P ＞ 0.05).p16 was found both in nucleus and cytoplasm of cervical carcinoma,whose expression rate was 83.58％ (56/67),higher than 41.37％ (24/58) in CIN (x2 =24.85,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =42.61,P ＜ 0.05).Conclusion Expression of IMP3 and p16 is high in cervical carcinoma,maybe can be used as a potential biomarker of metastasis and progression.

Objective:To develop a simple and rapid HPLC method to determine the concentration and pharmacokinetics of gliqui-done in human plasma. Methods: Acetonitrile was used to precipitate plasma proteins directly and gliquidone was detected by an HPLC-fluorescence assay. The mobile phase consisted of acetonitrile-20 mmol·L-1 biphosphate potassium (80∶20, v/v, pH 2. 5). The flow rate was 1. 0 ml·min-1 . The excitation wavelength was 315 nm and the emission wavelength was 410 nm. Results:The line-arity of gliquidone in plasma was good from 0. 034 to 2. 2 μg·ml-1 and the quantitative lower limit was 0. 034 μg·ml-1 . The range of method recovery was 99. 23%-108. 11%, and that of extraction recovery was 85. 78%-88. 77%. The intra-and inter-day precision was both less than 10%. Both specificity and stability met the requirements. Gliquidone in plasma was determined using the above method after a single oral dose of 60 mg gliquidone tablets in 20 healthy volunteers, and then the pharmacokinetic parameters were ana-lyzed. The variation coefficients were close to or more than 50%. Conclusion:The method is simple, rapid and sensitive, and suit-able for the detection of gliquidone in a large quantity. Individualized administration of gliquidone should be considered due to the obvi-ous individual difference in pharmacokinetics.

Objective To study the effect of calcitriol combined with metformin in the treatment of polycystic ovary syndrome(PCOS)and its influence on serum monocyte chemotactic factor -1(MCP -1).Methods 96 patients with PCOS were randomly divided into the control group(48 cases)and the the observation group(48 cases).The two groups were treated with metformin tablets,0.25g/time,2 times/d.On the basis of the above treatment,the observation group was given calcitriol,0.25μg/time,1 time /d.The two groups were treated for 3 months.Before and after treatment,the number of common symptoms of PCOS(menstrual scarce,acne,hairy,ovarian volume increase,polycystic change)and the determination of serum MCP -1 changes were summarized.Results Before treatment,the menstruation, acne,hairy,ovarian volume increase,polycystic change in control group were 46 cases,21 cases,32 cases,19 cases, 47 cases,respectively,which were 18 cases,11 cases,18 cases,9 cases and 10 cases after treatment.Before treatment, the menstruation,acne,hairy,ovarian volume increase,polycystic change in observation group were 47 cases,25 cases, 29 cases,22 cases,45 cases,respectively,which were 10 cases,6 cases,10 cases,4 cases and 5 cases after treatment. After treatment,the two groups were treated with less menstruation,acne,hair,ovarian volume and polycystic change (χ2 =9.86,7.25,9.06,10.17,13.42;14.28,12.62,11.28,16.52,19.66,all P <0.05).And after treatment, which of the observation group were significantly better than the control group (χ2 =4.06,4.86,5.62,4.71,9.27,all P <0.05).The serum MCP -1 levels of the control group before and after treatment were (159.64 ±27.12)μg/L, (118.54 ±21.38)μg/L,which of the observation group were (162.57 ±29.06)μg/L,(82.59 ±10.72)μg/L.After treatment,the serum levels of MCP -1 in the two groups were significantly decreased(t =2.968 2,4.163 8,all P <0.05),and the serum MCP -1 level in the observation group was significantly lower than that in the control group (t =2.661 2,P <0.05).Conclusion Calcitriol combined with metformin can significantly improve the efficacy of patients with PCOS,and decrease the expression of MCP -1 in serum.

OBJECTIVE: To study the pharmacokinetics and bioavailability of pulse controlled-release tablets of ketoprofen(KRT) in dogs.METHODS: A total of 6 dogs were randomly assigned to receive KRT(trial group,n=3) or KP enteric-coated capsules(control group,n=3) 150 mg,then a crossover trial was conducted 1 week later.Plasma samples were taken at different time points and the plasma concentration of ketoprofen in dogs was determined by HPLC.The pharmacokinetic parameters of the two KP preparations were computed and the correlation between absorption percentage in vivo and release rate in vitro was compared.RESULTS: The pharmacokinetic parameters of KRT versus those of its control preparation were as follows: tmax:(5.00?0.41) vs.(3.75?0.00) h;Cmax:(15.40?1.94) vs.(20.73?1.72) ?g?mL-1;AUC0～t(101.33?17.49) vs.(93.21?25.03) ?g?h?mL-1.The relative bioavailability of the trial preparation was 108.71%.The test preparation showed a good correlation(r=0.987) between absorption percentage in vivo and release rate in vitro.CONCLUSIONS: The KRT and KP enteric-coated capsules were bioequivalent.

Abnormity of spirit, mind and intelligence are related to phlegm heat disturbing orifices. This article listed several diseases about phlegm heat disturbing orifices through three medical records, expounded the mechanism of applyingGuntan Pills, and proposed the matters needing attention to using the medicine, with a purpose to promote wider application ofGuntan Pills in clinic.