Objective To investigate the prevalence of thalassemia gene carried by child-bearing age people in Meizhou area. Methods Use regular blood tests and hemoglobin electrophoresis to screen the suspect thalassemia patients in couples of child-bearing age who underwent premarital examination or pregnancy check in the hospital.Samples with positive results were tested by using hybridization reverse dot blot to make a definite diagnosis of thalassemia.Results 208 samples were screened to be positive, among which there were 64 cases ofα-thalassemia carrier(30.67%),32 cases ofβ-thalassemia carrier(15.38%),3 case of combina-tion thalassanemia(1.92%).Conclusion The study may provide a reference for taking effective measures to prevent the birth of children with thalassemia.

Objective To detect the expression of insulin-like growth factor Ⅱ mRNA-binding protein 3 (IMP3) and p16 in cervical carcinoma and investigate their clinical significance.Methods Immunohistochemical Envision method was used to detect the expression of IMP3 and p16 proteins in 67 cases of carcinoma of the uterine cervix,58 cases of cervical intraepithelial neoplasia (CIN) and 15 cases of normal cervical epithelium.Results IMP3 was mainly dedected in the cytoplasm,whose positive expression rate was 85.07％ (57/67) in cervical carcinoma,12.07％ (7/58) in CIN (x2 =66.32,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =44.38,P ＜ 0.05).No significance variations of the expression of IMP3 were observed in cervical adenocarcinoma and squamous cell carcinoma (x2 =0.35,P ＞ 0.05).p16 was found both in nucleus and cytoplasm of cervical carcinoma,whose expression rate was 83.58％ (56/67),higher than 41.37％ (24/58) in CIN (x2 =24.85,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =42.61,P ＜ 0.05).Conclusion Expression of IMP3 and p16 is high in cervical carcinoma,maybe can be used as a potential biomarker of metastasis and progression.

Objective:To develop a simple and rapid HPLC method to determine the concentration and pharmacokinetics of gliqui-done in human plasma. Methods: Acetonitrile was used to precipitate plasma proteins directly and gliquidone was detected by an HPLC-fluorescence assay. The mobile phase consisted of acetonitrile-20 mmol·L-1 biphosphate potassium (80∶20, v/v, pH 2. 5). The flow rate was 1. 0 ml·min-1 . The excitation wavelength was 315 nm and the emission wavelength was 410 nm. Results:The line-arity of gliquidone in plasma was good from 0. 034 to 2. 2 μg·ml-1 and the quantitative lower limit was 0. 034 μg·ml-1 . The range of method recovery was 99. 23%-108. 11%, and that of extraction recovery was 85. 78%-88. 77%. The intra-and inter-day precision was both less than 10%. Both specificity and stability met the requirements. Gliquidone in plasma was determined using the above method after a single oral dose of 60 mg gliquidone tablets in 20 healthy volunteers, and then the pharmacokinetic parameters were ana-lyzed. The variation coefficients were close to or more than 50%. Conclusion:The method is simple, rapid and sensitive, and suit-able for the detection of gliquidone in a large quantity. Individualized administration of gliquidone should be considered due to the obvi-ous individual difference in pharmacokinetics.

ObjectiveTo explore the clinical application of pleural mesothelial cell (PMC) determination in the diagnosis of pleural effusion.MethodsPMC of 36 patients with malignant pleural effusion (malignant group) and 24 patients with benign pleural effusion (benign group) were counted to determine the clinical value of PMC in benign and malignant pleural effusion.ResultsIn malignant group,there was no case with PMC disappear,1 case(2.78％) with the percentage of PMC between 0 and 1％,1case(2.78％) between 1％ and 5％,34 cases (94.44％) more than 5％.In benign group,there were 11 cases (45.83％) with PMC disappear,8 cases (33.33％) with the percentage of PMC between 0 and 1％,2 cases (8.33％) between 1％ and 5％,3 cases( 12.50％ ) more than 5％.The percentage of PMC between two groups had significant difference (x2 =43.4069,P ＜ 0.01).95％ confidence intervals of PMC in benign and malignant group respectively were 0.28％ -5.10％ and 5.13％ -10.91％,which did not overlap.The percentage of PMC more than 5％ was defined as the diagnosis standard of identifying benign and malignant pleural effusion,the sensitivity of the diagnosis was 94.44％(34/36),the specificity was 87.50％(21/24) and the accuracy was 91.67％(55/60).ConclusionsPMC determination is a kind of easy,economical and fast way to diagnose benignand malignant pleural effusion with high sensitivity,specificity and accuracy.Therefore,it has reference value in clinical diagnosis.

Abnormity of spirit, mind and intelligence are related to phlegm heat disturbing orifices. This article listed several diseases about phlegm heat disturbing orifices through three medical records, expounded the mechanism of applyingGuntan Pills, and proposed the matters needing attention to using the medicine, with a purpose to promote wider application ofGuntan Pills in clinic.

Objective To investigate the clinical characteristics ,diagnosis and prognosis of primary small intestine tumor . Methods Retrospective analysis was performed on the clinical data collected from the 47 cases of primary small intestine tumor . Results The clinical characteristics were non‐remarkable ,the preoperative diagnosis rate was 34 .0% (16/47) .Six cases (12 .8% ) were benign tumor ,adenoma was the main pathological type and the prognosis was good .Thirty‐three cases (70 .2% ) were malig‐nant tumor ,manifested mainly as anemia ,adenocarcinoma was the main pathological type ,originated from the duodenum mostly and the prognosis was poor .The 1 ,2 ,3 and 5 year survival rates of 26 cases in small intestine adenocarcinoma were 67 .4% ,42 .4% , 29 .4% and 22 .8% ,respectively ,and the median survival time was 20 .3 months .Eight cases(17 .0% ) were gastrointestinal stromal tumor ,most originated from the jejunum ,abdominal mass was the mainly clinical feature ,targeted drugs could help to improving the prognosis .Conclusion The incidence of primary small intestine tumor is low ,but most of them are malignant and the prognosis is poor .Find lesion in early stage with effective examinations and undergoing surgical resection are the key of treatment .

GATA4 gene mutations are common in children with congenital heart disease.More than 120 mutations have been found in ventricular septal defect,atrial septal defects and the tetralogy of fallot.But the relationship between genotype and clinical phenotype has not yet been elucidated.This article summarizes the published germline and somatic mutations of GATA4 gene in human congenital heart disease,and provides insight into the phenotypic spectrum of GATA4 gene mutation.

WJ multipurpose guided puncture outfit in interventional ultrasound was developed.Guided puncture with this outfit was performed on 203 cases of various diseases.All 274 punctures were saccessful The study shows hat this guided puncture outfit can be set on various linear probes,fit-ted into puncture,biolpsy or catheter needles.It can actively adjust the puncture angle,precisely forecast,fix and calibrate the puncture angle and depth,perlorm guided puncture in real time etc.It is notnecessary to set line and tabulate the puncture angle.The outfit is easy and rapid to handle and also is reliable with high success rate

OBJECTIVE: To study the pharmacokinetics and bioavailability of pulse controlled-release tablets of ketoprofen(KRT) in dogs.METHODS: A total of 6 dogs were randomly assigned to receive KRT(trial group,n=3) or KP enteric-coated capsules(control group,n=3) 150 mg,then a crossover trial was conducted 1 week later.Plasma samples were taken at different time points and the plasma concentration of ketoprofen in dogs was determined by HPLC.The pharmacokinetic parameters of the two KP preparations were computed and the correlation between absorption percentage in vivo and release rate in vitro was compared.RESULTS: The pharmacokinetic parameters of KRT versus those of its control preparation were as follows: tmax:(5.00?0.41) vs.(3.75?0.00) h;Cmax:(15.40?1.94) vs.(20.73?1.72) ?g?mL-1;AUC0～t(101.33?17.49) vs.(93.21?25.03) ?g?h?mL-1.The relative bioavailability of the trial preparation was 108.71%.The test preparation showed a good correlation(r=0.987) between absorption percentage in vivo and release rate in vitro.CONCLUSIONS: The KRT and KP enteric-coated capsules were bioequivalent.

Objective To study the effect of calcitriol combined with metformin in the treatment of polycystic ovary syndrome(PCOS)and its influence on serum monocyte chemotactic factor -1(MCP -1).Methods 96 patients with PCOS were randomly divided into the control group(48 cases)and the the observation group(48 cases).The two groups were treated with metformin tablets,0.25g/time,2 times/d.On the basis of the above treatment,the observation group was given calcitriol,0.25μg/time,1 time /d.The two groups were treated for 3 months.Before and after treatment,the number of common symptoms of PCOS(menstrual scarce,acne,hairy,ovarian volume increase,polycystic change)and the determination of serum MCP -1 changes were summarized.Results Before treatment,the menstruation, acne,hairy,ovarian volume increase,polycystic change in control group were 46 cases,21 cases,32 cases,19 cases, 47 cases,respectively,which were 18 cases,11 cases,18 cases,9 cases and 10 cases after treatment.Before treatment, the menstruation,acne,hairy,ovarian volume increase,polycystic change in observation group were 47 cases,25 cases, 29 cases,22 cases,45 cases,respectively,which were 10 cases,6 cases,10 cases,4 cases and 5 cases after treatment. After treatment,the two groups were treated with less menstruation,acne,hair,ovarian volume and polycystic change (χ2 =9.86,7.25,9.06,10.17,13.42;14.28,12.62,11.28,16.52,19.66,all P <0.05).And after treatment, which of the observation group were significantly better than the control group (χ2 =4.06,4.86,5.62,4.71,9.27,all P <0.05).The serum MCP -1 levels of the control group before and after treatment were (159.64 ±27.12)μg/L, (118.54 ±21.38)μg/L,which of the observation group were (162.57 ±29.06)μg/L,(82.59 ±10.72)μg/L.After treatment,the serum levels of MCP -1 in the two groups were significantly decreased(t =2.968 2,4.163 8,all P <0.05),and the serum MCP -1 level in the observation group was significantly lower than that in the control group (t =2.661 2,P <0.05).Conclusion Calcitriol combined with metformin can significantly improve the efficacy of patients with PCOS,and decrease the expression of MCP -1 in serum.