Replacement therapy of stem cells transplantation represents a potential treatment for neural retinal diseases.Despite the encouraging results in laboratory,the clinical application of cells replacement therapy is still difficult because the limitation of seed cells,immunologic rejection,oncogenicity and ethical problems,etc.Recent breakthrough in somatic reprogramming provides a promising solution overcoming these obstacles.Further researches on virus-free reprogramming will make the clinical application of stem cell replacement therapy possible.

ObjectiveTo investigate the psychological state and care measures for patients undergoing pereutaneous coronary intervention (PCI). Methods300 patients treated with PCI from December 2009 to June 2011 in our hospital were chosen as the research object.The factors arousing psychological disorders for PCI patients were taken for comparison.And then the appropriate nursing measures were discussed. ResultsThe most evident negative emotion of PCI patients was depression.The factors arousing negative emotion included the factors of family members and undesirable environment by many people going in and out. ConclusionsEffective measures can avoid the psychological barriers of PCI patients and improve the quality of postoperative recovery for patients.

OBJECTIVE: To explore the materials and selection of esophageal stents, and to discuss the application of covered stants in treating esophageal malignant stenosis.METHODS: CNKI and Medline databases were retrieved by computer for papers published from 1980 to 2009 with search terms of "esophageal carcinoma, esophageal stenosis, esophageal stent, and fabric-covered metallic stent". The language was limited for Chinese and English. A total of 61 iteratures were initial searched by computer, according to inclusive and exclusive criteria, 29 literatures were selected by the first author. Problems including esophageal stent category, material selection, as well as application of covered stents in treating esophageal malignant stenosis were reviewed and summarized.RESULTS: Totally 29 literatures were included in this paper. No concealed allocation or blinding method was described in the literatures. According to preparing materials, the stents could be divided into stainless metal stent, memory metallic stent and polyester plastic stent. As tissue-engineered materials, stents could be assigned into biodegradable polymer matrix stent and non-degradable biodegradable polymers stent. Each material stent possesses distinct advantage and disadvantage. The covered stents could relief esophageal stenosis effectively, interrupt tumor growth, and prevent restenosis. CONCLUSION: As a palliative therapy for treating esophageal malignant stenosis, covered stents can elevate patients' life quality and prolong survival times.

Objective:To evaluate the in vitro cytotoxicity,in vivo response and degradation of a new injectable nano-hydroxyapatite(n-HA)/calcium sulphate hemihydrate(CSH) bone substitute in rabbits and to discuss its possible bio-degeneration mechanism.Methods: Pure CSH,n-HA,10%n-HA+CSH,20%n-HA+CSH,and 40%n-HA+CSH(the last 3 containing 10%,20%,and 40% n-HA,respectively) were subjected to MTT assay for their in vitro cytotoxicities.20%n-HA+CSH was implanted into rabbits' muscle and bone defects in the right femur,and the general conditions of rabbits,the pathological and TEM manifestations of tissues,and the imaging alterations were all observed 5 days,2,3,4,6,8,and 12 weeks after implantation.Results: Test of cytotoxicity showed that the average cell proliferation rate was over 77% after treated with the solutions of 5 bone substitutes(P

Objective To evaluate the curative effect of Xuezhikang Capsules. Methods 92 cases of primary hyperlipidemia were randomly separated into experiment group and control group, Members in the experiment group were treated by Xuezhikang Capsules with 2 granules for one time and twice daily, lasting for 8 weeks. Members in the control group were treated by lovastatins after supper with 20mg for one time and once daily, lasting for 8 weeks, too. At the end of therapeutic courses, clinical efficacy of blood fat controlling was evaluated. Results There was no significant difference (P＞0.05 )between the two groups in terms of blood fat improvement and total effective rate. Conclusion Xuezhikang Capsules was effective in controlling hyperlipidemia.

0.05). Pretreatment with intrathecal prazosin (30 ?g) significantly reduced the antinociception of ketamine 10 min after injection but not 10?g (P

Objective:This study was to investigate the interrelations between the acetylase activity and specialization rate of the differentiation of MSCs to the cardiac cells when histon deacetylase enzyme inhibitor trichostatin A (TSA)interferes in the cardiac cells. Method: MSCs of ratswere separated,The already labeled MSCs and cardiac muscle cells were put respectively into the MSCs interfered by different concentrations of TSA cultivated in mix tune with cardiac muscles. Adding TSA into the conventional culture of MSCs for the negative group.One week later,with the help of immunofluorescence,detecting the expression of the function proteinum,ie. myosin heavy chain (MHC)and Connexin43 (Cx43),of the cardiac muscle constitution in MSCs was detected. Results: ① There appeared sample differentiation of the cardiac muscle cells after the coculture of the MSCs and the pulsating cardiac muscle cells for one week,and the expression of the function proteinum of the cardiac muscle constitution;② In the positive group,in the mix culture of the cardiac muscle cells and the MSCs in which the TSA interfered for 48 h,there appeard sample differentiation of the cardiac muscle cells;③ In the negative group,part of the MSCs could express the function proteinum of the cardiac muscle constitution. During the 7 days of coculture, with the induction of TSA,MSCs was double-labeled by Brdu-DAPI. The differentiation rate of the fluorescent manifestation of the MHC and Cx43 in the MSCs was notably different expression that in the positive group,Conclusion: MSCs in which HDAC,the enzyme inhibitor,interferes are in the microenvironment of the cardiac muscle cells. HDAC,the enzyme inhibitor,functions as a promotion factor in the specialization of MSCs,suggesting the interrelation between the acetylase activity and the cell specialization rates.

Objective:To explore whether phonocardiogram exercise test(PCGET) or doppler echocardiography can sensitively and specifically detect the changes of cardiac reserve in patients with hypertension and whether there is a relationship between the two methods.Methods:The trail was performed with eighty volunteers, including twenty healthy persons as control group,and sixty hypertensive patients, which were divided into three subgroups:hypertension A, HA(patients without left ventricular hypertrophy,LVH and enlarged left ventricle);hypertension B, HB(patients with only LVH) and hypertension C,HC(patients with both LVH and enlarged left ventricle).The cardiac contractility reserve index(CCRI) was measured by PCGET, while the value of LV end-systolic and end-diastolic long and short axis dimensions, wall thickness and synchronous blood pressure were measured by the technique of simultaneous screen display of electrocardiography and synchronously monitoring blood pressure.According to the parameters measured by the latter technique, the values of maximum elasticity(Emax), the average maximum myocardial elasticity stiffness(maxEav) and ejection fraction(EF)were calculated by computer programmed process.Results:(1)Compared with control group,there was a statistically significant difference between the hypertension groups except HA group,and also an obvious difference between HB and HC about the indexes of Emax, maxEav and EF(P

Objective To propose an electrocardiogram(ECG) feature extraction method,which is able to reflect the difference of the importance existing among the classes and at the same time to overcome the deficiency of conventional feature extraction method that is unable to solve the classification problem with priority.Methods The data for analysis was obtained from MIT-BIH database,including 250 samples each of normal sinus rhythm(NSR),atria premature contraction(APC),premature ventricular contraction(PVC),ventricular tachycardia(VT),ventricular fibrillation(VF) and super-ventricular tachycardia(SVT).The projecting vectors were constructed following the introduction of separable measurement to extract the features for the classification with priority.Results The proposed feature extraction method increased the average accuracy by 12 percentages as compared with conventional linear discriminative analysis(LDA) methods.Conclusion The prior classes can be better discriminated from others and separate each of the classes as much as possible at the same time.

Objective:Currently the treatment model of lumbar intervertebral disc herniation(LIDH) has changed from single surgical treatment to multisubjective comprehensive treatment.The purpose of this study was to observe the curative effect of synergic therapy with traditional Chinese medicine and physical therapy on LIDH.Methods:86 cases of LIDH treated with drawing,massage,Chinese herbal medicine injection and TDP radiation.Results:All the patients were followed for 1-7 months(mean 3 months).Convalescence 56 cases(65.1 %),effective 28 cases(32.6 %),no effect 2 cases(2.3 %),the total effective rate was 97.7%.Conclusion:The synergic therapy with traditional Chinese medicine and physical therapy was easy,safe and effective for LIDH.