1.Stem cells from childrens’ teeth
A.B. Mohd Hilmi ; S.N. Fazliah ; A. Siti Fadilah ; H. Asma ; A.R. Siti Razila ; S. Shaharum ; S. Jaafar ; A.B. Asiah ; O. Shamsuria
Archives of Orofacial Sciences 2008;3(1):29-31
The aim of this study was to isolate stem cells from dental
pulp of primary molars and incisors to be used as possible source for tissue engineering. Human primary molars and incisors were collected from subjects aged 4-7 year-old under standardized procedures.
Within 24 hours, the tooth was cut at the cemento-enamel junction using hard tissue material cutter. The dental pulp tissue was extracted, digested and then cultured in Alpha Modified Eagles’s Medium (α-MEM) supplemented with 20% FCS, 100 mM L-ascorbic acid 2-phosphate, 200 mM L-glutamine and 5000 units/ml Penicillin/Streptomycin. The cells were observed daily under the
microscope until confluence. Children’s tooth pulp- derived progenitor cells were found positive for stem cell markers CD105 and CD166, which are consistent with the finding for mesenchymal stem cells (MSCs) from bone marrow.
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