1.Mucinous adenocarcinoma arising from chronic perianal fistula.
Raja B Hisham ; A R Sabariah ; A G Yunus
The Medical journal of Malaysia 2006;61(1):88-90
Perianal mucinous adenocarcinoma is a rare tumor which may be associated with long-standing chronic perianal sepsis. Early diagnosis is challenging and is based on a high index of clinical suspicion and specific histological features. Definitive treatment is surgical, in the form of an abdomino-perineal resection. We hereby describe a case of a perianal mucinous adenocarcinoma arising from long-standing recurrent perianal fistula and complement this with a brief review of the literature pertaining in particular to the management of this condition.
Chronic
;
Fistula
;
Adenocarcinoma, Mucinous
;
Adenocarcinoma
;
Definitive
2.MTHFR C677T Polymorphism, Homocysteine and B-Vitamins Status in a Sample of Chinese and Malay Subjects in Universiti Putra Malaysia
Choo SC ; Loh SP ; Khor GL ; Sabariah MN ; Rozita R
Malaysian Journal of Nutrition 2011;17(2):249-258
Introduction: Methylenetetrahydrofolate reductase (MTHFR) C677T is involved
in folate and homocysteine metabolism. Disruption in the activity of this enzyme
will alter their levels in the body. Methodology: This study assessed MTHFR
C677T polymorphism and its relationship with serum homocysteine and Bvitamins
levels in a sample of Chinese and Malays subjects in UPM, Serdang.
One hundred subjects were randomly selected from among the university
population. Folate, vitamin B12, B6, and homocysteine levels were determined
using MBA, ECLIA, and HPLC, respectively. PCR coupled with HinfI digestion
was used for detection of MTHFR C677T polymorphism. Results: The frequency
of T allele was higher in the Chinese subjects (0.40) compared to the Malay (0.14).
Folate, vitamin B12 and B6 levels were highest in the wild genotype in both
ethnic groups. Subjects with heterozygous and homozygous genotype showed
the highest homocysteine levels. The serum folate and homocysteine were mainly
affected by homozygous genotype. Conclusion: MTHFR C677T polymorphism
plays an important role in influencing the folate and homocysteine metabolism.
3.Evaluation of DNA and RNA Extraction Methods
C S Edwin Shiaw ; M S Shiran ; Y K Cheah ; G C Tan ; A R Sabariah
The Medical Journal of Malaysia 2010;65(2):133-137
This study was done to evaluate various DNA and RNA
extractions from archival FFPE tissues. A total of 30 FFPE
blocks from the years of 2004 to 2006 were assessed with
each modified and adapted method. Extraction protocols
evaluated include the modified enzymatic extraction
method (Method A), Chelex-100 extraction method (Method
B), heat-induced retrieval in alkaline solution extraction
method (Methods C and D) and one commercial FFPE DNA
Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2
extraction protocols were evaluated including the
enzymatic extraction method (Method 1), and Chelex-100
RNA extraction method (Method 2). Results show that the
modified enzymatic extraction method (Method A) is an
efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.