Intraclass correlation coefficient (ICC) measures the extent of agreement and consistency among raters for two or more numerical or quantitative variables. This review paper aimed to present several tables that could illustrate the minimum sample sizes required for estimating the desired effect size of ICC, which is a measurement of the magnitude of an agreement. Determination of the minimum sample size under such circumstances is based on the two fundamentally important parameters, namely the actual value of the ICC and the number of observations made by each subject. The sample size calculations are derived from Power Analysis and Sample Size (PASS) software where the alpha and minimum required power is fixed at 0.05 and higher than 0.80 respectively. A discussion on how to use these tables for determining sample sizes required for each of the various scenarios and the limitations associated with their use in each of these scenarios is provided.

Issue：By 15 June 2012, States Parties to the International Health Regulations (2005), or IHR (2005), were required to have established the core capacities required to implement Annex 1 of IHR (2005).Context：The Pacific is home to 10 million people spread over 21 Pacific island countries and territories. Seven of those have populations of less than 25 000 people; 14 of the 21 Pacific island countries and territories are States Parties to the IHR (2005).Action：The World Health Organization Division of the South Pacific embarked on an initiative to support Pacific Island States Parties meet their 15 June 2012 IHR obligations. We adapted the 2012 IHR Monitoring Questionnaire (IHRMQ) to assist Pacific island countries and territories determine if they had met the capacities required to implement Annex 1 of the IHR (2005). If a Pacific island country or territory determined that it had not yet met the requirements, it could use the assessment outcome to develop a plan to address identified gaps.Outcome：Direct support was provided to 19 of 21 (91%) Pacific island countries and territories including 13 of 14 (93%) States Parties. Twelve of 14 (86%) fulfilled their requirements by 15 June 2012; those that had not yet met the requirements requested extensions and submitted plans describing how the IHR core capacities would be met.Discussion：Adapting the 2012 IHRMQ for this purpose provided an efficient tool for assessing national capacity to implement Annex 1 of IHR (2005) and provided clear indication of what capacities required strengthening.

Background Erythroderma is a serious condition in itself, quite apart from hazards associated with the underlying disease, and is sometimes fatal. Prognostic studies are rare in the literature and to date there are no published studies to identify the factors that can determine good prognosis. Objectives The aim of the study is to determine the factors that can prognosticate the good outcome of erythroderma. Methodology Cross sectional study from patients diagnosed to have erythroderma between 2003 and 2007 were analyzed with regard to age, sex, race, underlying medical illness, aetiology, duration of rash before diagnosis of erythroderma, response to topical therapy and prognosis. Results Four variables (aetiology, gender, duration of rash before being diagnosed as erythroderma and response to topical therapy) were associated with good prognosis. These variables were statistically significant from univariate analysis. When these variables were included into the binary logistic model, the study did not have enough evidence to proof that ‘aetiology’ and ‘gender’ can determine good prognosis. Response to topical therapy and shorter duration of rash (equal and less than 120 days) were significant with odds ratio (CI) of 4.11 (1.556, 10.885) and 4.608 (1.903, 11.155) respectively. Conclusion Shorter duration of rash and response to topical therapy are important factors to determine a good prognosis.

Replacing a single missing anterior tooth can be a challenge. Many factors need to be considered when choosing the appropriate treatment. Several treatment options are well established. This case report is to present the use of a cast cobalt chrome partial denture with custom made porcelain tooth to improve aesthetic. The overall shade of the artificial tooth were nicely matched with the adjacent teeth and definite enamel translucency could be achieved which would not be possible with acrylic or even with readymade porcelain tooth.

Background: Vertical jump is an index representing leg/kick power. The explosive movement of the kick is the key to scoring in martial arts competitions. It is important to determine factors that influence the vertical jump to help athletes improve their leg power. The objective of the present study is to identify anthropometric factors that influence vertical jump height for male and female martial arts athletes. Methods: Twenty-nine male and 25 female athletes participated in this study. Participants were Malaysian undergraduate students whose ages ranged from 18 to 24 years old. Their heights were measured using a stadiometer. The subjects were weighted using digital scale. Body mass index was calculated by kg/m2. Waist–hip ratio was measured from the ratio of waist to hip circumferences. Body fat % was obtained from the sum of four skinfold thickness using Harpenden callipers. The highest vertical jump from a stationary standing position was recorded. The maximum grip was recorded using a dynamometer. For standing back strength, the maximum pull upwards using a handle bar was recorded. Multiple linear regression was used to obtain the relationship between vertical jump height and explanatory variables with gender effect. Results: Body fat % has a significant negative relationship with vertical jump height (P < 0.001). The effect of gender is significant (P < 0.001): on average, males jumped 26% higher than females did. Conclusion: Vertical jump height of martial arts athletes can be predicted by body fat %. The vertical jump for male is higher than for their female counterparts. Reducing body fat by proper dietary planning will help to improve leg power.

Objective:To identify the differential expression of candidate gluconeogenic genes which may initiate hepatitis C virus (HCV) related metabolic disorder during early stages of disease. Methods:Patients of diverse age and sex, with positive HCV genotype 3 (HCV-3) RNA in serum and with no history of other related infections, co-infections, alcoholism, diabetes or chemotherapeutic treatments were considered for this study. Semi-quantitative reverse transcriptase PCR analysis and quantitative fold change analysis of the fresh liver biopsies of eight chronically infected HCV-3 patients and six healthy individuals were evaluated for three potential biomarkers involved in glucose homeostasis induction, namely mitochondrial phosphoenolpyruvate carboxykinase 2 (PCK2), glucose-6-phosphatase catalytic subunit (G6PC) and associated forkhead box protein 01 (FOXO1).Results:Symptomatic evaluation, clinical history and blood test were conducted according to general disease prognosis procedures and reported here. Significantly upregulated expression ofPCK2 independent of age, sex and viral infectivity levels in all HCV patients was observed, whereas no significant changes in the expression ofG6PC andFOXO1were found.Conclusions:PCK2 triggers initial gluconeogenic reactions which ultimately result in the accumulation of glycogen in the liver hepatocytes. We therefore suggest that the overproduction of PCK2 has important physiological role in the onset of metabolic disorder in the HCV-3 patients.

There have been a number of recent developments in the treatment of castration-resistant prostate cancer which seek to exploit the hormonal axis. Still, the castration-resistant prostate cancer remains a major challenge since this is the lethal and incurable phenotype which results in tens of thousands of deaths every year. There has been emerging interest in utilizing anticancer immunotherapy in prostate cancer, especially since the development of sipuleucel-T. Several other prostate cancer therapeutic vaccines including autologous and allogeneic vaccines, as well as viral vector-based vaccines, have demonstrated promising results in early trials. The checkpoint inhibitors which have shown some dramatic results in other cancers are now being studied in advanced prostate cancer setting. Studies are examining the therapeutic effects for both CTLA-4 inhibitors and PD-1/PD-L1 inhibitors. It appears that definitions and measurements of response used in cytotoxic therapies may not be valid in determining response to immunotherapy. Early reports suggest that combination therapies, either concurrent or sequential, may be needed to achieve the desired response against advanced prostate cancer.

In a few years we are likely to see 3D images generated instantly, and with comparable resolution to today's 2D views. Inclusion of functional information, possibly at the molecular level, could also assist in clinical decision-making. Some specialist clinicians with intimate knowledge of their field of interest are likely to have a better understanding of the pathology and physiology of an organ system than a general radiologist. So given that the images will be presented in a more familiar format, why should clinicians and surgeons wait for a general radiologist to read them? If radiologists wish to retain their role as the experts in image interpretation, they will not only need a thorough understanding of imaging and radiological anatomy, but also a detailed understanding of pathology and physiology. It is clearly unrealistic to expect most people to gain that knowledge across a range of fields, hence the need for subspecialization. There are already commercial moves to harness the expertise of superspecialist radiologists, using teleradiology, to provide expert opinions in particularly difficult cases. This is just the beginning of a major shift in the pattern of practice in radiology. The radiology community cannot ignore impending technological developments. If radiologists take no interest in the emergence of highly detailed, user-friendly images, then the clinicians and surgeons will organise their own department-based image interpretation. However, radiologists are very good at adapting to technological change and are very likely to rise to these challenges. Far from having a sell-by date, radiology has a bright future.
Radiology ; standards ; trends ; Time Factors

Objective To explore the relationship between WWOX gene and attachment and adhesion of ovarian cancer. Methods The expression of WWOX mRNA was detected by RT-PCR, the expression of the WWOX protein was evaluated by western blot in WWOX-transfected PEO1 cells (H6, H7, H8 cell) and vector-transfected control cells (vec-1, vec-2 cell). Attachment assay was used to assess the adhesion of the tranafection in PEOI cells via culturing the cells on the pre-coated fibronectin wells. RNA interference (RNAi) was used to knockdown the endogenous expression of WWOX in the A2780 ovarian cancer cell line by liposome. Attachment assay was detected the adhesion to fibronectin after gene silencing. Restdts RT-PCR showed that expression of mRNA WWOX in exon9 was in all transfection cells (H6, H7, H8, vec-1, vec-2 cell ). Western blot showed that expression of WWOX protein was in the WWOX-transfected cells (H6, H7, H8 cell ), but not in the vector-transfected cells (vec-1, vec-2 cell ). Attachment assay showed that H6, H7, H8 cell (0.098±0.003, 0.091±0.004, 0.099±0.003) adhered more slowly to fibronectin than vec-1, vec-2 cell (0.185±0.003, 0.175±0.006) and non-transfected PEO1 cell (0.211±0.007), and demonstrated significantly reduced adhesion after 2 hours (P ＜ 0.01). A2780 adhesive cells that WWOX gene be knockdown was 0.059±0.005, adhered more significantly rapid than those untreated cells that was 0.029±0.003 after treated 30 minutes (P ＜ 0.05 ). Conclusions WWOX gene can suppress adhesion to fibronectin in ovarian cancer cells. This suggests an important role for loss of WWOX gene in promoting attachment and adhesion of ovarian cancer cells on loco-regional peritoneum, and further resulting in enhancing loco-regional peritoneal tumor invasiveness and spread.