No abstract available.
Hemoglobins

Background: Anemia is a major global problem that affects women and prevalent during pregnancy. Effective management is needed to prevent adverse maternal and pregnancy outcomes. Ferrous iron salts are the preparation of choice and recommended for both prevention and treatment of iron deficiency anemia (IDA). However, most commonly available iron supplement are poorly absorbed, with gastrointestinal disturbances as side effect. Objective: To compare the efficacy of iron amino acid chelate and ferrous sulfate in the treatment of IDA among pregnant women seen at the out-patient department of a tertiary medical center. Methodology: This study is a single blind randomized clinical trial which included women 18 to 40 years old, with singleton pregnancies diagnosed with IDA without any co-existing fetal and maternal complications seen at the OutPatient Department. Forty eight eligible participants were randomized, with 24 women allocated on each treatment arm who took their assigned treatment twice a day for 90 days. Hemoglobin, hematocrit, MCHC, MCV, RDW & serum ferritin levels were taken at baseline and monitored on days 30, 60 and 90 from initiation of treatment. Mean blood parameters between two treatment arms were compared on days 30, 60 and 90 post-treatment as well as the mean difference of blood parameters on days post-treatment from the baseline using T-test. Chi-square was used to compare adverse effects between two treatment arms. Results: No statistically significant differences in the mean blood parameters on days 30 and 60 of treatment between Iron amino acid chelate and Ferrous sulfate. It was only on day 90 from initiation of treatment when there were a significantly higher hematocrit and MCHC and lower RDW in Iron amino acid chelate compared to Ferrous sulfate group. All of the CBC parameters on days 30, 60, and 90 post-treatment when compared to baseline level were significantly increased for both treatment arms. However, day 90 level of serum ferritin in the Iron amino acid chelate group significantly increased unlike those in ferrous sulfate group. Conclusion Iron amino acid chelate is comparable to Ferrous sulfate in the treatment of IDA among pregnant women. Iron amino acid chelate was found to be superior to Ferrous sulfate in achieving optimum treatment response even at a lower dose with lesser adverse effects. Hence, better oral iron treatment tolerability, thereby, compliance to long-term therapy can be expected resulting to successful treatment outcome.
Hemoglobins ; Hematocrit

BACKGROUND: We evaluated the HbA1c assay on COBAS INTEGRA 800 Closed Tube System (Roche Diagnostics, USA). METHODS: Precision was determined following Clinical and Laboratory Standards Institute (CLSI) EP5-A2 using the Lyphochek Diabetes Control (Bio-Rad Laboratories, Canada). Two levels of QC materials were assayed in duplicates at two separate times per day for 20 days. The within-run, between-run and between-day precisions were evaluated. We compared the HbA1c of COBAS INTEGRA 800 Closed tube system using Tina quant Hemoglobin A1c Gen.2 (Roche Diagnostics, Germany) reagent with Variant II Turbo (Bio-Rad Laboratories, USA). Bias was determined following CLSI EP9-A2. RESULT: The HbA1c assay on COBAS INTEGRA 800 Closed Tube System showed excellent precision performance: at a level of 5.9%, total, between run, and between day CVs were 1.8%, 0.5%, and 1.6%, respectively. At a level of 9.6%, total, between run, and between day CVs were 1.4%, 0.3%, and 1.1%, respectively. The assay correlated well with the Variant II Turbo (y=0.9x+0.53, r2=0.9857). Mean bias against Variant II Turbo was -0.2%. Compared with Variant II Turbo, the estimate of the predicted bias at a given medical decision level (HbA1c at 6% and 9%) was -0.1% and -0.31%, respectively. CONCLUSIONS: The COBAS INTEGRA 800 Closed Tube System HbA1C assay was precise and equivalent to Variant II Turbo.
Bias (Epidemiology) ; Hemoglobins

Background: Hemoglobin is a composition of erythrocyte. They are released from the lysis of erythrocyte. Objectives: to study free hemoglobin concentrations in surgical patients received massive blood transfusion (MBT). Subject and methods: 48 adult patients (male: 31 (64.6%), female: 17 (35.4%), mean age: 36 \xb1 16) with more than 3000ml of storage blood were tested for plasma free hemoglobin. The major reason for MBT were severe trauma (45.8%), the remainder were non - trauma. The change of Hb free was followed in 16 patients at the times of 1500 ml, 2000 ml, 3000 ml storage blood transfused (9 \xb1 3 days). Results: Plasma free hemoglobin was detected after 2,000ml of red cell transfusion and during the first 24h post MBT in 100% cases. Plasma free hemoglobin concentration trended to increase with quantity of transfused blood (0.56 \xb1 0.3 g/dl after 2000 ml, 0.76 \xb1 0.47 g/dl after 3000 ml, and 0.93 \xb1 0.41 g/dl after 5000 ml). There was no correlation between free hemoglobin concentration in plasma of patients with the storage time of blood transfused, and no difference of plasma free hemoglobin concentration between in trauma and non trauma patients. Conclusion: The blood with short time of storage is recommended after 1500ml blood transfusion in these patients. \r\n', u'\r\n', u'
Blood Transfusion ; Hemoglobins/ pharmacokinetics

On studying 147 hemoglobin electrophoresis samples of the Stieng community, we refound that hemoglobin varieties distributed as following: - HbE: 54.45% including HbE homozygous: 6.12%; HbAe: 41.5% and -thal/HbE: 6.8%.- -thal: only -thal/HbE (-thal/HbE: 4.08%, -thal/HbE, 2.72%). -Normal cases: 45.58%. These results shown that HbE ratio is very height in Stieng community, it is necessary to have the special investigations to find out the causes
Hemoglobins ; Minority Groups ; Electrophoresis

Totally 179 children aged from 6 months to 15 years from 2 villages Taja and Na Cang, Than Uyen district, Lao Cai province was investigated clinically and hematologically. Among them, 99 were boys and 80 girls. The results show that prevalance of anemia among these children was as high as 49.7%. 50 anemic from children 89 had hemoglobin disorder. All children with moderate and severe anemia had hemoglobin-diseases. From the result above we suggest that thalassemia and HbE are serious cause for anemia in children from the region. It is nesecery to establish the thalassemia prevention programme for this region.
Hemoglobins ; Anemia ; Child

This longitudinal prospective study was carried out on 6 blood samples of 6 male volunteers at National Institute of Hematology and Blood Transfusion to examine the changes biochemical parameters, shape of blood cells, and variations of all blood components during blood store. Results: plasma Hb and K+ concentrations and LDH activity in stored blood increased significantly; plasma Na+ and Cl concentrations decreased significantly along the time. These variations occurred in the first week of store. There were changes in the shape of red blood cells during blood store.
Blood ; Hemoglobins ; Plasma ; Electrolytes

No abstract available.
Erythropoiesis ; Hematinics ; Hemoglobins

Background: Anemia is the most common medical disorder throughout a woman’s life. Given that anemia during reproductive years impacts future gestational and perinatal health, then any factors possibly associated with the disorder should be studied and ultimately addressed. Objective: This study aimed to determine anemia prevalence and its associated factors (socio-demographic, lifestyle, knowledge, dietary and biochemical) among a select group of women of reproductive age (WRA) living in Tondo, Manila. Methods: This is an analytic cross-sectional study that utilized interviewer-assisted questionnaire and blood extraction to collect data from 166 WRA (18-40 years). Results: Anemia prevalence among the study participants was found to be 9.0%. In terms of associated factors, being anemic was found to be more likely if the WRA has these factors: higher parity, graduated from college; unemployed; living in a low-income household; took iron supplements, and; have low knowledge on iron. On the other hand, increase in age, increase intake of heme iron and non-heme iron food sources, and an increase in blood ferritin concentration were factors that made anemia less likely among WRA. Conclusion Examining these factors provides insight into the multicausal nature of anemia. Thus, prevention and control programs should focus on the contribution and interrelatedness of these factors to address anemia.
Female ; Iron ; Reproduction ; Hemoglobins

BACKGROUND: Hemoglobin A1c (HbA1c) is a useful marker for the diagnosis and monitoring of diabetes, which has resulted in an increasing dependency on HbA1c levels for diagnosing diabetes in small- and medium-sized hospitals. We evaluated a high performance liquid chromatography (HPLC) based HbA1c autoanalyzer Bio-Rad D-10 (Bio-Rad Laboratories, USA) by comparing the analysis results with that by Tosoh HLC-723 G7 (Tosoh Corporation, Japan). METHODS: The Bio-Rad D-10 autoanalyzer was evaluated for its precision, linearity, and carryover. The analysis time and correlation were evaluated and compared with those by Tosoh HLC-723 G7 autoanalyzer. RESULTS: Bio-Rad D-10 showed within-run, between-day, and total precision of less than 1.3% coefficient of variation (CV) and excellent linearity between HbA1c in the range of 3.2%-21% (coefficient of determination, R 2 = 0.998). The sampleto-sample carryover was 0.57%. The results obtained by using Bio-Rad D-10 showed good correlation (r = 0.997; P < 0.001) with those by Tosoh HLC-723 G7; however, the analysis time using Bio-Rad D-10 was about 2.3 times per 10 samples and 2.5 times per 20 samples than those using Tosoh HLC-723 G7. CONCLUSIONS: Bio-Rad D-10 showed good performance in assaying HbA1c. Bio-Rad D-10 autoanalyzer would be suitable for use in laboratories with small to medium amount of samples to be analyzed, but its initial analyzing time was longer than that by Tosoh HLC-723 G7.
Chromatography, Liquid ; Dependency (Psychology) ; Hemoglobins