OBJECTIVETo elucidate the characteristics of genetic variability and its relationship with prevalence, through sequencing and analysis of N gene among street rabies virus isolated from different hosts (homo sapiens, ferret badger, dog) in Zhejiang province.

METHODSSamples were screened and confirmed by direct fluorescence assay and reverse transcript PCR. Sequences were analyzed using bio-information software.

RESULTSEighteen street rabies virus strains were identified, including 2 from homo sapiens, 5 from ferret badger, and 11 from dog. Similarities of N gene and N protein were calculated to be 89.7%-100.0% and 98.4%-100.0% respectively. Mutations occurred in N gene were almost non-sense mutations. In addition,Data from phylogenetic analysis showed that all these strains could be classified into traditional genotype 1.

CONCLUSIONThe prevalence of rabies viruses among different hosts in Zhejiang province had certain regional properties. Rabies viruses isolated from the same kind of host or from the same/adjacent county/counties had the closest relationship. However, the characteristics of rabies virus prevalent in homo sapiens were somewhat complicated. In summary, the transmission of street rabies virus in Zhejiang province was from dogs to ferret badgers and homo sapiens, and the virus could circulate and cross-regional transmit among dogs and ferret badgers.

Animals ; China ; epidemiology ; DNA Mutational Analysis ; Dogs ; virology ; Humans ; Mustelidae ; virology ; RNA, Viral ; genetics ; Rabies ; epidemiology ; Rabies virus ; genetics ; isolation & purification ; Viral Envelope Proteins ; genetics

Genes, Viral ; Rabies virus ; classification ; genetics ; Serotyping

Epidemic cerebrospinal meningitis (meningococcal meningitis) is an acute respiratory infectious disease with high mortality and serious sequelae. Meningococcal vaccine is an effective measure to prevent and control meningococcal meningitis. At present, group B meningococcal meningitis has become the main prevalent serum group in the world, including China. Meningococcal ACYW and other vaccines are mainly composed of capsular polysaccharides, while the main component of group B meningococcal vaccine is protein, including outer membrane vesicles (OMV) and recombinant protein vaccine. The methods for evaluating the immunogenicity of group B meningococcal vaccine include hSBA and alternative methods such as meningococcal antigen typing system (MATS), flow cytometric meningococcal antigen surface expression assay (MEASURE), genetic meningococcal antigen typing system (gMATS) and bexsero antigen sequence type (BAST). The evaluation of vaccine immunogenicity is the basis of vaccine development and clinical trial research, However, at present, there is no group B meningococcal vaccine in China. Therefore, in this paper, the research progress of immunogenicity evaluation of group B meningococcal vaccine has been reviewed, in order to provide technical guidance for the research and development of group B meningococcal vaccine, immunogenicity evaluation and clinical trial research in China.
Humans ; Meningitis, Meningococcal/prevention & control* ; Meningococcal Vaccines ; Neisseria meningitidis ; Serogroup ; Vaccines, Combined

The incidence of meningococcal disease is generally low globally at present. The epidemics, problems and challenges of meningococcal disease were described in order to provide support for prevention and control of meningococcal disease in China, especially in the areas of disease surveillance, epidemic changes, serogroup witching, vaccines and vaccination strategies and meningococcal group B vaccine development.

OBJECTIVETo understand the epidemiological characteristics of head injuries through data from the Chinese National Injury Surveillance System (NISS).

METHODSDescriptive analysis was applied to display the overall trend of head injuries in 2013 in NISS and to depict general information, events and clinical characteristics of head injuries with SPSS 19.0 software.

RESULTSIn 2013, 195 189 cases of head injuries were collected, males were twice higher than females, with 25.19% of them under 30-44 years of age. The three leading causes responsible for head injuries were falls (42.17%), blunt force injuries (27.46%) and road traffic injury (23.33%). Main locations causing head injuries were in road/street (31.41%), at home (25.02%) and public places (17.17%). Recreation activates (54.22%), driving (19.73%), paid work (12.95%) were the three major activities when injuries took place. Majority of the cases belonged to unintentional (86.79%) with bruise injuries (65.18%). Those mild injuries (78.87%) were treated and discharged (82.02%).

CONCLUSIONIn 2013, head injuries were seen more in males than in females, mostly involved in labor force population on head injuries. The leading causes for head injuries were falls, blunt injuries and road traffic.

Accidental Falls ; statistics & numerical data ; Accidents, Traffic ; statistics & numerical data ; Adult ; Automobile Driving ; China ; epidemiology ; Craniocerebral Trauma ; epidemiology ; Employment ; Environment ; Female ; Humans ; Male ; Population Surveillance ; Software ; Wounds, Nonpenetrating ; epidemiology

OBJECTIVETo understand the trend and characteristics of sports and recreation related injuries reported from National Injury Surveillance System (NISS) to provide basis for corresponding prevention strategies and decision-making.

METHODSDescriptive analysis was applied to display the overall trend, general information, injury event and clinical characteristics of sports and recreation related injuries from 2009 to 2013.

RESULTSThe proportion of sports and recreation related injuries among all injuries increased from 2009 to 2013, with an annual increase exceeding 45% (46.21%, 47.32%, 48.14%, 52.00%, 53.65%, respectively). Sports and recreation related injuries mainly involved males, with 15-29 age groups, particularly in summer and autumn. Sports and recreation related injuries mostly occurred at home, with annual rates of proportion as 33.07%, 34.16%, 32.98%, 34.57 and 36.22%, mostly caused by falls (41.19%, 41.64%, 44.70%, 47.41%, 47.96%). Contusion and abrasion were the leading types of injuries (43.49%, 44.56%, 45.14%, 45.02%, 45.62%) with the serious leading types as fracture, concussion/cerebral contusion or laceration, and sharp force injury/bite/open wounds. Head was the part mainly involved (31.30%, 32.48%, 31.89%, 30.88%, 29.44%) in injuries. Most sports and recreation related injuries were minor and most of the patients headed home after treatment.

CONCLUSIONSports and recreation related injury appeared a growing public health problem in China. Children and the elderly should be the target groups for intervention. Falls prevention in sports and the use of protection gears should be the focus countermeasures for prevention.

Adolescent ; Adult ; Athletic Injuries ; epidemiology ; Brain Injuries ; epidemiology ; China ; epidemiology ; Female ; Humans ; Male ; Population Surveillance ; Recreation ; Wounds and Injuries ; epidemiology ; Young Adult

OBJECTIVETo evaluate the performance of national injury surveillance in China and provide evidence for the utilization and interpretation of surveillance data and the improvement of injury surveillance.

METHODSAccording to the national injury surveillance protocol, a retrospective analysis was conducted on the quality of injury surveillance carried out by the centers for disease control and prevention (CDCs) at all levels in China from 2006 to 2012. And related human resource and budget data in 2012 were collected for analysis.

RESULTSFrom 2006 to 2012, the injury cases reported to national injury surveillance system increased by 1 time in China. The underreporting rate of injury cases and mis-filling rate of reporting cards decreased in 31 surveillance points (72.1%) and 23 surveillance points (53.5%) respectively. In 2012, the underreporting rates were less than 10% in 33 surveillance points (76.7%) , and the mis-filling rates were less than 10% in 39 surveillance points (90.7%) . Only 19 provincial CDCs (44.2%) and 32 county/district CDCs had full time staff engaged in injury surveillance. Three surveillance points (7.0%) never published their injury surveillance data, while 13 surveillance points shared injury surveillance data with other departments.

CONCLUSIONThe quality of injury surveillance was greatly improved in China during 2006-2012, but more efforts are still needed for the further improvement of national injury surveillance, including the increase of human resources and fund investments and more use of surveillance data.

OBJECTIVETo analyze the trend and distribution of Falls, using the data from National Injury Surveillance System (NISS), 2008-2013, to provide basic information for Fall prevention.

METHODSData of Falls was descriptively analyzed from the Chinese NISS from 2008 to 2013.

RESULTSIn total, 1 223 905 cases of Falls were analyzed, that accounted for 32.94% of all the injury cases, with the gender ratio as 1.57. Both the number and the proportion of the cases showed an increasing trend. Labor population (15-64 years-old) accounted for 66.63% of the total 271 502 cases of Falls in 2013. Cases aged 0-4, 5-14 and over 65 accounted for 55.53%, 52.93% and 56.59% among all the types of injuries in each of their age group. Gender ratio appeared to be 1.54 but more females cases were seen with the increase of age. The peak hours that injuries took place were 10:00-10:59 AM (10.04% of total) and 14:00-20:59 PM (43.56% of total). The top three places that injuries took place were home (33.03%), public residents (22.79%) and on the roads (15.49%). Recreation activates (65.38%), paid jobs (13.01%) and housework (7.52%) were the three major ways causing the Falls to take place. The most common parts of body that injuries involved were head (30.41%), low limbs (27.43%) and upper limbs (21.93%), with bruise (54.60%), sprain/strain (16.87%), fractures (15.48%) most commonly seen. 77.76% of the cases were minor but the proportion of moderate and serious injuries increased along with the increase of age.

CONCLUSIONFall was the main type of injury in this country, showing an increasing trend of proportion among all the types of injuries. Though labor-related Falls accounted for the biggest proportion, prevention programs targeting the elderly and children should also be prioritized.

Accidental Falls ; prevention & control ; statistics & numerical data ; Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Middle Aged ; Wounds and Injuries ; epidemiology

Humans ; Measles ; prevention & control ; Measles Vaccine

Objective: To identify whether the three imported yellow fever cases in China in March 2016 were infections by wild type strain of yellow fever virus in Angola in 2016, vaccine-associated disease or co-infection of both. Methods: Sequences of three yellow fever virus strains were obtained by high-throughput sequencing with IonTorrent PGM platform from blood or urine samples of three yellow fever cases, and their genomic characteristics were analyzed. Then the regions with relatively great difference between the wild type strain and 17D vaccine strain were identified, and then served as the reference sequences when mapping the reads obtained by high-throughput sequencing. Results: Partial yellow fever virus genomes were obtained from three samples of yellow fever patients, among them a full length coding region sequence was gained in sample 2. Comparing the genome sequences, the three newly obtained strains of yellow fever virus were highly similar to strain CNYF01R / 2016 which was isolated from the first imported yellow fever case to China in 2016 and strain Angola 71 from Angola in 1971, and they all belonged to Angola genotype of yellow fever virus. In this study, we found five regions in yellow fever virus genomes with great diversity between the vaccine strain and the wild type strain. In these five regions, a number of short reads obtained by high-throughput sequencing of the three samples were mapped to the sequence of wild type virus, while no short reads matched the vaccine strain. Conclusions There were no viral nucleic acid of 17D vaccine strain in the blood or urine samples of these three cases of yellow fever. They are all infected by wild type strains of Angola in 2016.