Objective To investigate the study of acute toxicity of mon-golian medicine Meng gen wu sen ri le, to understand the dose of acute toxicity and distribution and death of acute toxicity after the administra-tion, to determine lethal dose 50 ( LD50 ) of Meng gen wu sen ri le. Methods According to the results of preliminary experiments, using Conn synthesis method ( modified Karber method ) , divided into six groups dosage are 14.30, 9.28, 6.04, 3.92, 2.55, 1.66 g? kg-1 , af-ter experiments observing 14 day, to record the change of the weight and adverse reaction.Results LD50 of Meng gen wu sen ri le is 5.1597 g? kg-1 ( 95%CI:3.6652-7.2637 g? kg -1 ).In 14 days, it does not appear obvious symptoms of adverse reaction and body weight has growth trends.Conclusion According to the results of experiments, LD50 of Meng gen wu sen ri le is 100 times of the clinical dosage, tip single oral is safer.

Proteomic tools were used to identify the key proteins that might be associated with bronchial asthma(BA). Firstly, the serum samples from healthy adults and asthmatic patients were collected. Tandem Mass Tag~(TM)(TMT), which removes high-abundance structures and nonspecific proteins, was employed to identify the differentially expressed proteins between asthmatic patients and healthy adults. Gene Ontology(GO) annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were carried out for the differentially expressed proteins. The core proteins in the asthma group were screened out by protein-protein interaction(PPI) analysis. Then the core proteins were verified by Western blot for 3 patients with bronchial asthma and 3 healthy adults. A total of 778 differentially expressed proteins were screened out, among which 32 proteins contained quantitative information, including 18 up-regulated proteins and 14 down-regulated proteins. The differentially expressed proteins were enriched in 28 KEGG signaling pathways. The PPI analysis showed that 10 proteins(GDN, 1433 Z, VWF, HEMO, CERU, A1 AT, TSP1, G3 P, IBP7, and KPYM) might be involved in the pathogenesis of bronchial asthma. Compared with those in healthy adults, the expression levels of SLC25 A4, SVEP1, and KRT25 in the sera of asthmatic patients were up-regulated(P<0.05). Therefore, it is hypothesized that a variety of immune signaling pathways and differentially expressed proteins play a role in the pathogenesis of BA, which provides potential target information for the treatment of BA.
Adult ; Humans ; Proteomics ; Gene Ontology ; Proteins ; Disease Susceptibility ; Asthma/genetics*