Background: Oxidative damage culminates in diabetic macrovascular complication. Ethanolic extract propolis of Heterotrigona itama exhibits antioxidative, antidiabetic and cardioprotective properties. However, vasoprotective activity has yet to be reported. This research was designed to investigate the effects of the ethanolic extract of propolis on histology and oxidative stress parameters in the aorta of diabetic rats. Methods: Raw propolis from Kelantan bee farm was ground, dissolved and distilled with ethanol into crude extract. Adult male Sprague Dawley rats were divided into four groups, n=8/group: normoglycemia (NG), untreated diabetes (DM), metformin-treated diabetes (DM+M, 300 mg/kg/day) and propolis-treated diabetes (DM+P, 300 mg/kg/day). A single injection of intraperitoneal streptozotocin (60 mg/kg) was used to induce diabetes mellitus, followed by a four-week treatment, via oral gavage, administered immediately after successful diabetes-induction with evidence of hyperglycaemia. Both NG and DM groups were given one millilitre of the vehicle. Rats were sacrificed at the end of the experiment; blood was collected for determination of serum lipid profile; the aorta was fixed and processed for histomorphology and histomorphometry evaluation; aortic tissue homogenate assay for oxidant/antioxidant activities. Differences in the parameters between groups were analysed using one-way analysis of variance (ANOVA) with post-hoc adjustment. Reults: Propolis treatment elevated the high-density lipoprotein level and suppressed protein oxidation in diabetic rats. Furthermore, propolis halted the thickening of tunica intima and aberrant proliferation of vascular smooth muscle cells in tunica media. Conclusion In conclusion, this proof-of-concept study indicated the potential of propolis as a vasoprotective agent against the early pathogenesis of diabetic macroangiopathy through anti-oxidative and anti- hyperglycaemic activities.
Antioxidants

Radix Polygoni multiflori has been used Vietnamese traditional medicine for a long time. This paper presented the remarkable antioxidant effects of the AcOET extract from Radix Polygoni multiflori with evidences obtained from various antioxidant tests: inhibition of lipid peroxidation in cell hemogenates, of anion superoxide production, stimulation of SOD activity using Ransod kits. The extract protected the splenocytes from H20¬2- induced injuries and protected brain cells from oxidative stress caused by an ischemia/reperfusion course. These results suggested that application of Radix Polygoni multiflori as antioxidant agent will be beneficial in prevention and treatment of diseases of the elderly
Acetates ; Antioxidants

Free radical and antioxidant are very interesting field of research in other country as in Viet Nam. They are mentioned as a new pathogennic agent. Objectives: Determination of total antioxidant capacity in plasma. Methods: Lag-time assay was established to measure Total Antioxidants Capacity (TAC) in human plasma of healthy volunteers (6 males, 4 females, aged 29,8+-5,2). Results: - TAC was 513.3+/-79.64 mM Trolox equivalents. TAC was significantly higher in men (568.83+/-41.08 mM Trolox equivalents) in comparison with women (430.00+/-28.15 mM Trolox equivalents). A main reason was a higher concentration of uric acid in men compared with women (397.5+-79.86 mM versus 245+-33.16 mM). Conclusions: TAC in plasma after drinking vegetable juice for 1 week (547.8+-80.67 mM Trolox equivalents) was significantly higher in comparison with before (513.3+-79.64 mM Trolox equivalents).
Antioxidants, Plasma

Hygrocybe conica (HC), a wild mushroom commonly consumed by the indigenous people (Orang Asti) in Peninsular Malaysia, was assessed for its antioxidant content. Methods: The HC mushroom was extracted using distilled water and the crude extract partitioned using different solvents and open column chromatography to evaluate its potential antioxidant properties. The mushroom extract was partitioned using liquid-liquid extraction into the hexane (F1), chloroform (F2), butanol (F3) and formic acid (F4) fractions. Based on solvent polarity, the water extract of the mushroom was fractionated into non-polar (FI), semi-polar (FII), and polar fractions (FIII) using open column chromato�graphy. Antioxidant capacities were determined using DPPH, ABTS, and ferric reducing antioxidant power (FRAP) assays while Folin-Ciocalteu reagent assay was used to determine total phenolic content (TPC). Results: The HC extract had the highest TPC and DPPH scavenging capacity compared to its extract fractions. TE values (ABTS assay) of F2 and F4 were not significantly higher than the HC extract. Among the extract fractions of different polarities, FIII had the highest antioxidant capacities (DPPH and FRAP) compared to FI and FII while FRAP values of these fractions were not significantly lower than the FRAP value of HC extract. The HC extract had significantly lower antioxidant capacity than antioxidant standards (ascorbic acid and BHA). Tannic acid as the main bioactive component in HC mushroom was detected using HPLC method. The presence of phenolics in HC extract was also confirmed using TLC. Conclusion: Due to the presence of potent phenolic components, the mycelia of HC could be consumed for potential antioxidative benefits.
Antioxidants ; Agaricales

Centella asiatica, locally well known in Malaysia as pegaga, is a traditional herb that has been used widely in Ayurvedic medicine, traditional Chinese medicine, and in the traditional medicine of other Southeast Asian countries including Malaysia. Although consumption of the plant is indicated for various illnesses, its potential neuroprotective properties have been well studied and documented. In addition to past studies, recent studies also discovered and/or reconfirmed that C. asiatica acts as an antioxidant, reducing the effect of oxidative stress in vitro and in vivo. At the in vitro level, C. asiatica promotes dendrite arborisation and elongation, and also protects the neurons from apoptosis. In vivo studies have shown that the whole extract and also individual compounds of C. asiatica have a protective effect against various neurological diseases. Most of the in vivo studies on neuroprotective effects have focused on Alzheimer’s disease, Parkinson’s disease, learning and memory enhancement, neurotoxicity and other mental illnesses such as depression and anxiety, and epilepsy. Recent studies have embarked on finding the molecular mechanism of neuroprotection by C. asiatica extract. However, the capability of C. asiatica in enhancing neuroregeneration has not been studied much and is limited to the regeneration of crushed sciatic nerves and protection from neuronal injury in hypoxia conditions. More studies are still needed to identify the compounds and the mechanism of action of C. asiatica that are particularly involved in neuroprotection and neuroregeneration. Furthermore, the extraction method, biochemical profile and dosage information of the C. asiatica extract need to be standardised to enhance the economic value of this traditional herb and to accelerate the entry of C. asiatica extracts into modern medicine.
Centella ; Antioxidants

No abstract available.
Antioxidants* ; Oxidative Stress*

Experimentally, hepatoprotective effect of DinhLang was expressed in mice. The extract of Dinh Lang leaf suppressed the increase of MDA level in brain and in liver of mice suffered from CCl4- induced hepatitis with antioxydant activity stronger than that of mixed extract and extract prepared from the root. Dinh Lang had manifested liver protective effect in tetrachlorurcarbone –induced acute hepatitis. Antioxydant effect expressed in the inhibition against the processes of peroxydation of cell membrane can be one of the mechanism of liver protective effect of Dinh Lang
Antioxidants ; Araliaceae ; liver

The study was performed on 26 AO/dioxin exposed patients in the war. Product Naturenz was used to prevent and reduce the damage of polluted substances, and to increase detoxifying process, antioxidant, improve health. Results: Antioxidant enzymes were improved after patients used naturenz with the dose of 2g/day in 2 months (GPX enzyme activity improvement accounted for 57.7%, SOD enzyme activity tented to increase to normal value which accounted for 38.5%, peroxidase activity improvement accounted for 61,8%). Especially, in total antioxidant activity, Hemoglobin content and SH-group content improved significantly (increase by 76.9%). Therefore, naturenz have antioxidant effect and improve health for the patients
Enzymes ; Antioxidants ; Dioxins

In 1969, Mc Cord and Fridovich discovered an enzyme that had an antioxidative activity – it was superoxide dismatase (SOD). In 1984, Cutler R.G determined the pathogenic roles of free radicals in biology. In 1986, Marman D mentioned the relationship of free radicals and origin and development of organism, relationship with pathogenesis and aging
Free Radicals ; Therapeutics ; Antioxidants

Antioxydant effect of radix notogingseng fluid extract 1:2 was studied in mice. Experiment mice were divided randomly into 3 groups x 10 animals. Control group (group 1) was given orally with NaCl 0,9% + oliver oil infiction, group 2 was given orally with NaCl 0,9% + CCl4 infection, group 3 with notogingseng extract 5g/kg of mice. Results showed the antioxydant effect of notogingseng at moderate level. It increased the liver GSH content in comparing with intocicated group.
Antioxidants ; Animal Experimentation ; Mice