Objective: The occurrence of food contaminants such as aflatoxin in the foodstuffs has been reported widely. Unfortunately, only a few know about the impact of aflatoxin to human health and this phenomenon let us to question the extent of public’s knowledge on fungal and aflatoxin contamination in the diets. Thus, this study aimed to investigate determinants of adults’ knowledge on fungal and aflatoxin contamination in the diets based on two factors namely socio-demographic and socio-economic factors. Method: A questionnaire was self-administered to 160 respondents from a faculty in Universiti Putra Malaysia. Results: Most of respondents had low level of knowledge in regard to fungal and aflatoxin contamination. Besides, the total score of knowledge on fungal and aflatoxin contamination was significantly and positively correlated (r=0.340, P<0.0001). The multivariate analysis indicated that personal income (below US $487) was the only predictor of respondent’s knowledge (β=-0.288, P<0.001) [Odds ratio (OR)=4.996]. Nonetheless, being male and single, divorced or widowed had significant OR of 2.040 and 0.313 respectively as predictors of low level of knowledge. Conclusions: Income inequalities may have impact to the respondents in acquiring knowledge on fungal and aflatoxin contamination in the diets. Additionally, an extensive survey on aflatoxin should be warranted in order to assess the public awareness and knowledge about this food contaminant.