Abstract: Since the ancient times Mongolian traditional medicine widely used a sheep’s fat tail. As sheep fat tail oil is natural originated pure product, which has high concentration of unsaturated acid and valuable treatment and nutritional efficiency, this study was conducted to identify the possibility to use sheep fat tail oil instead of cosmetic raw material. We prepared bodylotion, bodycream and bodybutter base from sheep fat tail and determined “pH” MNS 1474:2000, “Acid number” MNS 1131:2000, “Heat stability” MNS 4961:2000 and microbiological analysis. From our results it is shown that cosmetic products, meets the standard requirements. Key words: sheep fat tail, cosmetic products

Introduction: This plants distributed about 2500 species in worldwide and 69 species of those grow in Mongolia. In case of medicinal application of this plant is commonly used traditional medicine in European and Asian. For example, the different preparations of Astragalus mongholicus root, the most commonly used Astragalus species in Chinese medicine. Other Astragalus species which are being investigated for their anti-cancer properties are the Turkish species, and include Astragalus brachypterus, Astragalus cephalotes, Astragalus microcephalus and Astragalus trojanus. Nowdays, this plant source from nature is decreased and areal square de-escalated in Mongolia. Goals: Our main goal was conserves domesticates and improves plant genotypes of local wild medicinal specie and provides raw plant material for scientific testing from the cultivation site without depleting wild sources. Including that main goal we put a purpose of the make comparative study of vegetative organ’s anatomy morphological study on wild and planted type. Materials and Methods: The wild Astragalus mongolicus bunge was collected from Bayanchandmani sum, for Tuv province. Cropped plant was grown medicinal botanical garden of Drug research institute, Monos. Anatomy structure of vegetative organs was identified by plant anatomic method. Result: The article presents the data on structure of vegetative organs of cultivated Astragalus mongolicus Bunge and compared with wild type. Comparative studies were made on the leaf, root, stem and flower and the anatomical structure of vegetative organs of Astragalus mongolicus Bunge and wild type, which were the similar plants on botany and had the great value of medicine. The results indicated that the anatomical structures of their vegetative organs were basically the same, but there are some obvious differences on leaf and stem it depended from geographical influences. Planted plant in particular could alter their environment by affecting light and water avialablity, soil nutrient availability due to uptake. Discussion: Tserenkhand.G was studied Astragalus mongolicus root anatomy in 2003. Our root anatomy study was same to Tserenkhand.G. Conclusion: The results from this study, we are concluded that, the cultivated Astragalus mongolicus Bunge is greatly cultivated in our Botanical garden. Key words: cultivated, anatomy, epidermis

Introduction: Chamaenerion angustifolium (L.) Scop. has a cup-shaped nectary which locates in hollow receptacle, belonging to receptacle nectary. One layer of eqidermis on which modified stoma lie is covered by a thin cuticular layer. The nectary is differentiated from the srperfical layer cells of receptacle and that no special initial has been found. Goal: The aim of this study was to develop a natural Chamaenerion angustifolium (L.) Scop plant’s standardization requirements. Material and Methods: The root samples of natural Chamaenerion angustifolium (L.) Scop was collected from Umnudelger sum, Khentii aimag in August, 2013. The plant material dried under shade at room temperature. Then passed through 120 mesh size to remove coarse powder and fine powder was used for estimation of biologically active compound content in plant material [1, 3, 7]. Shimazdu UV –VIS Spectrophotometer was employed for all spectroscopic measurements using a pair of matched quartz cells and Shimadzu High Perpormance Liquid Chromatography equipment equipped with SPD - 20 A UV detector, CMB - 20 A system controller, CTO-10 AS vp column oven with injector and LC-6AD pumps. Gallic acid, Folin-Ciocalteau, sodium carbonate, methanol, sulfuric acid, acetic acid and glucose used were of the highest commercially available purity [8. 9, 10]. Results: The present study was carried out to develop a simple, rapid, sensitive, accurate, precise spectrophotometric and HPLC method to determine polysaccharide, polyphenolic and gallic acid by simultaneous estimation of in standardization formulation. Chromatographic analysis was carried out by Luna C18 (2) 100A reversed phase column (150 x 4.6 mm) packed with 5μm diameter particles. The mobile phase was 0.1% acedic acid:Methaol (95:5 v/v). The mobile phase was filtered through a 0.45 μm membrane filter. Then it was degassed ultrasonically prior to use. HPLC identification of standard gallic acid was at 278 nm. Flow rate and injection volume were 0.8 ml /min and 20 µl, respectively. Gallic acid was eluted with retention times of 8.2 min respectively. Amounts of gallic acid were 0.3% in plant. The standard deviation values were satisfactorily low and recovery was closed to 100% indicating the reproducibility, accuracy and precision of proposed method. The natural plant contents of the polysaccharid and polyphenolic was found 2.65% and 4.59%, respectively. Conclusion: The results from this study, we developed natural Chamaenerion angustifolium (L.) Scop. plant’s chemical ingredients for the its standardization. Keywords: Chamaenerion angustifolium (L.) Scop., polysaccharide, polyphenolic and gallic acid.

Introduction: Factors are increasing in Mongolia, which is producing food product using sea buckthorn berry. However, those factors use pulp oil, juice and throw skins, pulp and seeds. Those solid remains named pomace. The pomace contains many kinds of vitamins, trace elements and other biologically active substances including fat soluble vitamins /А, Е, К/, fatty acids / linoleic, linolienic, oleic/, carbohydrates, water soluble vitamins /С, В1, В2, folic acid/, flavonoids, carotenoids. Therefore, pomace is usable as raw material for biological active product. Goal: The aim of study is to invent sample of biological active product including seabackthorn pomace Materials and methods: The seabuckthorn pomace was provided by Monos Foods Co Ltd. Ascorbic acid and other expient were provided by Drug Research Institute. Sea buckthorn (H. rhamnoides) pomace was dried by Labconco freezone L12 freeze dryer in Drug Research Institute. Moisture and total ash were determined by method according to MNS 5225:2002, contents of total fat was determined by Soxhlet method, vitamin C and total acidity were analyzed by titremitric method, total flavonoid was analyzed spectrophotometric method and impurity of dry pomace was measured by The State Pharmacopoeia of the Russian Federation XI. Quality requirements of tablet, capsule and granule were determined by Mongolian National First Pharmacopeia. Total bacteria and fungal were determined by method in MNS 5194:2002 and MNS 5193:2002. Results: Dried seabuckthorn pomace was standardized according to MNS 5225:2002. The results indicated that dried seabuckthorn contains humidity 4.3%, total ash 2.16%, β-carotin 110.3%, fiber 31.78%, total flavonoid 0.25%, impurity 0.13%, acidity 2.06%, total fat 33.3%, total fungi count 3*102 and bacterial count 2*102, respectively. We created tablets 0.3 g contain dry pomace and determined quality and quantity parameters. Quality requirements of tablets were standardized and following results were obtained: the 0.3g tablet had an average weight 0.301g, the dissolution of the 0.3g tablet was 97.6%, disintegration 2.93 minute and ascorbic acid 30.66 mg. Furthermore, capsules contain dry pomace were created. Its average weight was 0.45g ±7.5%, disintegration 24 minute and β-carotine 0.12 mg, respectively. Finally, granule product contains vitamin C and dry pomace was created. In this granules content β-carotene 1.32 mg and vitamin C 145 mg, respectively. Conclusion: Quality requirements and microbial contaminants of dry seabuckthorn pomace were agreed with MNS 5225:2002. 0.45 g capsule, 0.3 g tablets, 5g granule were created. Those new products meet the general requirements for tablet; capsule and granule by Mongolian National First Pharmacopeia, and 0.45g capsule, 5g granule products were more suitable for production of biological active products. Key words: sea buckthorn pomace, (H. rhamnoides), biological active product

Introduction: The development of a plant derived supplement with mild effects and reduced toxic and side effects for the treatment of respiratory disease is very crucial. Therefore we tested the cough suppressant effects of three plant preparations that are made from Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisch and Vaccinium Vitis idea/, plants that are common in our country. Materials and Methods:The research was conducted in 2013-2014 at the Pharmacological Laboratory of the Drug Research Institute. The experiment was carried out on four groups of 20 guinea weighing 300-400 grams and the experimental condition of coughing was generated using the method of Ucelay et al. Result:The results of the study show that the combination 3 (Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisc, Vaccinium Vitis idea) had suppressed the most when compared to the other combinations.

Introduction: Throughout the world, there are a number of plants that have been identified with immune boosting ability and the following plants Salsola laricifolia Turcz, Inula helenium that have been proven to support the immune system and grow in Mongolia were selected for a phytochemical study and prepared technology of dried powder.Goal: To develop dry extract technology with immunity stimulating action from natural plant sources.Material and methods: A biological active substance coumarin and flavonoid determination byspectrophotometer.Result: Total coumarin in raw material of Salsola laricifolia was 2.9±0.03%, total flavonoid amount was 1.66±0.021%, total polysaccharide was 3.83±0.025%, humidity was 5.7±0.01%, extractive substance amount was 20.63±0.91% ( p≤0.05). Total polysaccharide in raw material of Inula helenium was 20.78±0.03%, humidity was 6.03±0.03%, extractive substances amount was 21.76±0.23.Salsola laricifolia’s polysaccharide content was the highest or 0.19±0.031%, when extracted with 30% ethanol the flavonoid content was 0.36% when 25%, 30% ethanol was used as the extragent, 30% ethanol is determined to be an appropriate extragent. Inula helenium’s water extract contained 3.75±0.05% polysaccharide, 0.43±0.005% flavonoid. 50% alcohol extract had a 3.20±0.01% polysaccharide, flavonoid content of 0.25±0.01% and water extragent is determined to be proper extragent in future research.The scheme of dry extract preparation technology was developed and determined quantitative indication. Total polysaccharide which was active substance of Salsola laricifolia’s 30% ethanol and microcrystal cellulose under 15:1 version was highest in 0.46±0.011% and total polysaccharide which was active substance of Inula helenium water extract and microcrystal cellulose under 10:1 version was highest in 3.46±0.021% that`s why, it is suitable to dry extracting Salsola laricifolia’s 30% ethanol and microcrystal cellulose under 15:1 version, Inula helenium water extract and microcrystal cellulose under 10:1 version.Key word: Inula helenium, Salsola laricifolia, immunity support, dry extract

Introduction: The World Health Organization (WHO) according respiratory allergic diseases, nasal mucosal disease , which is one factor to create asthma and more than 300 million people become infected is accured in 2012[1]. In 2012, acconding to statistics of the Ministry of Health for our country was noted of 5% of the total population of the allergic disease and astma.Also In 2000, the prevalence of astma among the population was 2.4%, but in 2010 increased by 4.8%. Accordingly , the prevalence of the disease is increasing year by year, that’s why the imported new drug’s of diseases in the market and the need to produce domestically. Domestic market, we used allergic rhinitis, mucosal inflammatory treatment cholorphenamine 4 mg (Monos Industry LLC), Tavegil (Novartis Russia ), ketotifen (So pharma, Bulgaria), letizine 10 mg (KRKa, Slovani), Cetirizine 10 mg (Bertecs pharma, Russian). Letizine 10 mg, Cetirizine 10 mg drugs are contain of Ceterizine dicholoride, widely used in the treatment of foreign drug. Divided in the medical treatment of children aged 2-6 years , these drugs are expected to give a child , so we import the drug entering the appropriate doses of the company with the goal to obtain. For that purpose we determination allergon tablet’s technological process. Conclusion: Technological experiment “Alergon 5 mg ” tablets were selected ingredients of ceterizine dihydrocholoride 0.005 g, glucose monohydrate 0.09444 g, K-30 0.00006 g, magnesium stearat 0.0005 g. According to our study, ”Allergon 5 mg” tablets average weight, weigth range, disintegration, hardness, assay meets the standards set in the Mongolian National Pharmacopeia requirement of “Tablet drug ”.

Introduction: Achillea asiatiсa has been used in traditional medicine for one hundreds of years. Many compounds have been isolated from /Achillea asiatica/, including flavonoids, sesquiterpenes and essential oils. Salsola collina herb grows in Siberia, Baikal and Altai. It contains a variety of amino acids, flavonoids, glycosides, and vitamins. It has a positive effect on metabolism of fats in liver, regulates cholesterol and sugar in the blood and is recommended for weight reduction. Salsola collina is widely distributed in droughty and semi-droughty area, which is used as a kind of folk remedy in traditional Chinese and Mpngolian traditional medicine for treatment of hypertension and liver protectevity . Goal: The aim of study is to choose optimal extragent and determination liver protecting activity in Achillea asiatiсa, Salsola colina and to determine main substances in these herbals. Material and Methods: Achillea asiatiсa was collected from Dorgonot mountain Tuv province in June of 2012, Salsola colina were collected from Medicinal botanical garden of Drug Research Institute in August of 2012. Maceration was chosen by suitable extraction method and optimal extragent was ethanol. Flavonoids were determined in evaporated extract of Achillea asiatiсa, Salsola colina using the method by Mongolian National first Pharmacopeia. We use non line wistar rats, determination liver protecting activity biochemical analysis by Hospitex analyzer. Result: Liquid extracts were obtained from Achillea asiatiсa, Salsola colina with maceration method. Liquid extracts were evaporated on Vacuum rotor. The tehnological parameters of liquid extracts are the main important index to calculate the extragent correctly, to establish the material balance exactly, and to extract the process efficiently. 70% ethanol (1:10 sample/extragent) was determined by optimal extragent for Achillea asiatiсa, 80% ethanol (1:10) for Salsola colina. Achillea asiatiсa, Salsola colina were have liver protecting activity. Conclusion: Some technological parameters of liquid extract were determined such as optimal extragent and the effective extracting method of Achillea asiatiсa, Salsola colina. As the result of determining these parameters, it has much practical significant for establishing the technological condition to extract the biological activity compounds completely from their plants. Key words: Achillea asiatiсa, Salsola collina, technological pharameters, flavonoid, anthraquinons, liver protecting activity

Introduction: Constipation is not often regarded as a major therapeutic issue, but the use and abuse of laxatives by older people is highly prevalent. It is important for clinical to have an understanding of constipation to people, and of ways to improve laxative use. The laxatives most appropriate for older people include stimulants such as senna, bulking agents. Cassia acutifolia L and Rheum undalatum L have been used as a laxative drug in Asian traditional medicine for a long time. The pods and leaves contain antraquinone aglycons and antraquinone glycosides which are laxative form while rhein is a major component. The anti constipation ability of combinations of plant preparations consisting of Cassia acutifolia, Rheum Undalatum, Terminilia Chebula Retz, and soda were studied. Anti constipation drug set for LD50 samples of. The pharmacological experiments were done on 30 rat of wistar species weighing 200 -250 grams and 30 rabbits of Shinshilla species weighing 2000-2200 grams, 60 mouse of Balb/c species weighing 20-32 grams. The combinations of plant were given to experimental animals orally with the dose of convenient. The experimental results indicate that sample 1 compared to other samples dungy moisture to add the most during pathological induced anti constipation. Goal: The aim of study is determination anti constipation activity in plant preparations. Material and methods: Rheum undalatum L was collected from Medicinal botanical garden of Drug Research Institute in September of 2011. Cassia acutifolia, Terminilia Chebula and Soda were to be out from Monos food LLC in August of 2013. Maceration was chosen by suitable extraction method and optimal extragent was ethanol. Antraquinone was determined in evaporated extract of Cassia acutifolia, Rheum undalatum using the method by Russian Pharmacopeia. The pharmacological experiments were done on 30 rat of wistar species weighing 200 -250 grams and 30 rabbits of shinshilla species weighing 2000 -2200 grams, 60 mouse of Balb/c specie weighing 20 -32 grams. The combinations of plant were given to experimental animals orally with the dose of convenient. The experimental results indicate that sample 1 compared to other samples dungy moisture to add the most during pathological induced anti constipation. Result: Liquid extracts were obtained from Cassia acutifolia L, Rheum undalatum L with maceration method. Liquid extracts were evaporated on Vacuum rotor. Sample 1 was have anti constipation activity Keywords: Cassia acutifolia L, Rheum undalatum L, Terminilia Chebula Retz, salt, rabbits, rats, mice, and Constipation

"Introduction A chromatographer always looks forward to getting perfect shaped peaks for each and every analysis but in reality peaks get distorted due to numerous reasons. Distortions are frustrating but if proper corrective steps are taken peak shape distortions can be avoided. Peak purity can be established by taking the ratio of two wavelengths across the peaks of running samples or standard. May, if the peak is pure, then the ratio of wavelengths should be constant across the peak. Otherwise, the peak is impure, then the ratio between two wavelengths will change across the peak as the spectral differences caused by interfering peak change the signal ratio."