"Introduction
A chromatographer always looks forward to
getting perfect shaped peaks for each and every
analysis but in reality peaks get distorted due to
numerous reasons. Distortions are frustrating but
if proper corrective steps are taken peak shape
distortions can be avoided. Peak purity can be
established by taking the ratio of two wavelengths
across the peaks of running samples or
standard. May, if the peak is pure, then the ratio
of wavelengths should be constant across the
peak. Otherwise, the peak is impure, then the ratio
between two wavelengths will change across
the peak as the spectral differences caused by
interfering peak change the signal ratio."