1.Genospecies Classification of Acinetobacter calcoaceticus-Acinetobacter baumannii Complex by Restriction Fragment Length Polymorphism.
Hyo Sun LIM ; Hyo Jung HONG ; Hyun Jung JO ; Do Hee KIM ; Kyung Soo CHANG
Journal of Bacteriology and Virology 2011;41(1):37-45
Acinetobacter calcoaceticus-Acinetobacter baumannii (A. calcoaceticus-A. baumannii) complex, which includes A. calcoaceticus (genospecies 1), A. baumannii (genospecies 2), Acinetobacter genospecies 3 and 13, has been identified as A. baumannii by automated bacteria identification system. The purpose of this study is to develop rapid genospecies classification of A. calcoaceticus-A. baumannii complex by molecular techniques. Random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) were determined for 4 reference strains and 80 isolates of A. calcoaceticus-A. baumannii complex from clinical sources. Four and eleven RAPD patterns were observed among the reference strains and the isolates, respectively. RAPD might be useful for genomic typing but not for genospecies classification of Acinetobacter spp. RFLP of 16S-23S rRNA intergenic spacer gene with three selected restriction enzymes (ApaLI, SwaI, and SalI) showed only four RFLP patterns in the reference and the isolates. Of 80 isolates, 10 of A. calcoaceticus (12.5%), 50 of A. baumannii (62.5%), 11 of A. genospecies 3 (13.75%), and 9 of A. genospecies 13 (11.25%) were classified by RFLP. This result suggests that RFLP of 16S-23S rRNA intergenic spacer gene of A. calcoaceticus-A. baumannii complex might be useful for genospecies classification.
Acinetobacter
;
Bacteria
;
DNA
;
Polymorphism, Restriction Fragment Length
2.Restriction fragment length polymorphism analysis in differentiating mycobacterium tuberculosis strains.
Tae Yoon LEE ; Seung Gu SHIN ; Sung Kwang KIM
Journal of the Korean Society for Microbiology 1992;27(2):155-161
No abstract available.
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymorphism, Restriction Fragment Length*
3.Restriction fragment length polymorphisms of Dx13/BgI II associated with factor VIII: C gene in Koreans.
Sung Ro CHUNG ; No Bum LEE ; Hyung MOON ; Chung Geun LEE ; Myung Soo LYU ; Chang Ryul KIM ; Hahng LEE ; Yong Seok KIM ; Jai Kyung KOH
Korean Journal of Obstetrics and Gynecology 1992;35(7):1038-1044
No abstract available.
Factor VIII*
;
Polymorphism, Restriction Fragment Length*
5.Development of Species-specific Primers for Rapid Detection of Phellinus linteus and P. baumii.
Mun Ok KIM ; Gi Young KIM ; Byung Hyouk NAM ; Cheng Yun JIN ; Ki Won LEE ; Jae Min PARK ; Sang Joon LEE ; Jae Dong LEE
Mycobiology 2005;33(2):104-108
Genus Phellinus taxonomically belongs to Aphyllophorales and some species of this genus have been used as a medicinal ingredients and Indian folk medicines. Especially, P. linteus and morphological-related species are well-known medicinal fungi that have various biological activities such as humoral and cell-mediated, anti-mutagenic, and anti-cancer activities. However, little is known about the rapid detection for complex Phellinus species. Therefore, this study was carried out to develop specific primers for the rapid detection of P. linteus and other related species. Designing the species-specific primers was done based on internal transcribed spacer sequence data. Each primer set detected specifically P. linteus (PL2/PL5R) and P. baumii (PB1/PB4R). These primer sets could be useful for the rapid detection of specific-species among unidentified Phellinus species. Moreover, restriction fragment length polymorphism analysis of the ITS region with HaeIII was also useful for clarifying the relationship between each 5 Phellinus species.
Fungi
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Phylogeny
;
Polymorphism, Restriction Fragment Length
;
Polyporales
6.Genetic diversity of germplasm resources of Lonicera japonica by AFLP analysis.
Qing-Mei GUO ; Ting WANG ; Feng-Qin ZHOU ; Jia LI ; Yong-Qing ZHANG
China Journal of Chinese Materia Medica 2012;37(20):3024-3028
OBJECTIVEThis study aimed to analyze the genetic diversity and genetic relationship of germplasm resources of Lonicera japonica in main producing areas of China and provide reference for developing new varieties of L. japonica.
METHODUsing 6 primer combinations, 13 germplasm of L. japonica were analyzed by AFLP marker. The genetic distance was worked out by using DPS V3.01 software, and the cluster was conducted based on UPGMA.
RESULTA total of 435 bands were obtained including 191 polymorphic ones. The average polymorphic frequency was 43.9%. Cluster analysis showed that the relationship of cultivated variety from the same genuine area was near, and the classification result based on AFLP marker of germplasm of L. japonica from Shandong province was basically consistent with those on their morphological character.
CONCLUSIONAFLP marker can indicate the abundant genetic diversity of L. japonica and provide theoretical evidence for reasonable utilization and breeding new cultivar of L. japonica in molecular level.
Amplified Fragment Length Polymorphism Analysis ; China ; Genetic Variation ; Lonicera ; classification ; genetics ; Phylogeny ; Polymorphism, Restriction Fragment Length
7.Restriction fragment length polymorphism analysis of varicella-zoster virus isolated in Korea.
Kyu Kye HWANG ; Song Yong PARK ; Sung Jin KIM ; Yeon Woo RYU ; Kyong Ho KIM
Journal of the Korean Society of Virology 1991;21(2):201-210
No abstract available.
Herpesvirus 3, Human*
;
Korea*
;
Polymorphism, Restriction Fragment Length*
8.IS6110 - RFLP Analysis using Mycobacterium tuberculosis Isolates from Taegu - Kyungpook Region.
Jong Seok LEE ; Sung Kwang KIM ; Jai Youl LEE ; Tae Yoon LEE
Journal of Bacteriology and Virology 2001;31(1):29-37
No abstract available.
Daegu*
;
Gyeongsangbuk-do*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymorphism, Restriction Fragment Length*
9.Molecular genetic study of St14.1(DXS52) TaqI RFLPs in Koreans for the diagnosis of hemophilia A.
Chun Geun LEE ; Myung Soo LYU ; Youl Hee CHO ; Yong Kyun PAIK ; Sung Ro CHUNG ; Jung Hwan SHIN ; Hyung MOON
Korean Journal of Obstetrics and Gynecology 1992;35(11):1605-1612
No abstract available.
Diagnosis*
;
Hemophilia A*
;
Molecular Biology*
;
Polymorphism, Restriction Fragment Length*
10.Carrier detection of hemophilia A by PCR/RFLP (polymerase chain reaction/polymorphism) based analysis of the intron 18
Journal of Practical Medicine 2003;458(8):58-61
Establish DNA polymorphism methods (PCR/RFLP) with marker Bcl1 restriction enzyme on intron 18 for detecting mothers who carried an abnormal X chromosome with hemophilia and transfer to their sons. 4 of 9 families carried intron 18-related mutations of hemophilia A and these mothers transfered mutations to their sons to cause hemophilia patients
Hemophilia A
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length