Ischemic stroke is a cerebrovascular disease with high incidence and disability rates. Non-coding RNAs， as important regulatory factors for gene expression， play a key role in the development and progression of ischemic stroke， but their specific mechanisms of action remain unclear. This article systematically reviews the expression characteristics and regulatory roles of microRNAs， long non-coding RNAs， and circular RNAs in ischemic stroke and reveals the pathophysiological mechanisms of non-coding RNAs in ischemic injury by regulating the processes of cell apoptosis and autophagy， inflammatory response， blood-brain barrier integrity， and neuroregeneration. In addition， non-coding RNAs have shown the potential as biomarkers for the prediction， diagnosis， and prognostic evaluation of ischemic stroke. This article also analyzes the limitations of current research and proposes future research directions， so as to provide a theoretical foundation for exploring the mechanism of action of non-coding RNAs in ischemic stroke and developing innovative diagnostic and therapeutic strategies.
Review

Strengthening comprehensive quality and safety management is great significance in improving the level of medical services,ensuring patient safety and enhancing medical efficiency.On the basis of discussing the significance and role of building a smart medical quality management system,it has sorted out the key paths of data integration and sharing,data analysis and mining,intelligent decision-making support,operation optimisation and efficiency enhancement,information security and privacy protection,talent cultivation and digital literacy enhancement,and put forward the suggestions to jointly promote the construction of the management system from the aspects of policy guidance,multi-party collaboration,and technological innovation.

Traditional Chinese medicine,ancient Greek medicine,Ayurvedic medicine,and Arab medicine are recognized as the four major traditional medicines in the world.It reviews the education and training systems of the four major traditional medicines and finds that traditional Chinese medicine focuses on the teacher-student relation-ship and the combination of theory and practice;Ancient Greek medicine was mainly characterized by strong theoreti-cal research and experimental observation;Ayurveda highly values cultural identity as its main characteristic;Arab medicine attaches great importance to cultural exchange and practical promotion.It suggests promoting innovative de-velopment,strengthening practical teaching,improving teaching quality,strengthening international exchanges and cooperation,and increasing public acceptance abroad.

Nonalcoholic fatty liver disease(NAFLD)has become the most common liver disease in the world and is an important risk factor for the progression to hepatocellular carcinoma.However,the pathogenesis of NAFLD remains unclear,and there is still a lack of specific treatment measures.Sterol regulatory element-binding proteins(SREBP)are an important nuclear transcription factor,which mainly maintains the balance of lipid metabolism inside the body by activating the genes associated with the synthesis and uptake of cholesterol,fatty acids,and triglycerides,and therefore,SREBP are a target for the treatment of metabolic diseases.This article reviews the latest advances in SREBP in the pathogenesis of NAFLD and the latest evidence of SREBP-targeted therapy for NAFLD.It is worth noting that recent studies have shown that SREBP inhibition can cause liver injury together with autophagy damage.Therefore,excessive inhibition of lipogenesis may exert a counterproductive effect on the treatment of NAFLD.In conclusion,SREBP is a promising therapeutic target for NAFLD;the molecular mechanism of SREBP in lipid metabolism is regulated by many factors,and these factors are being deeply explored and analyzed,which has an important clinical significance for the treatment of NAFLD.

Objective To construct the competency of traditional Chinese medicine(TCM)rehabilitation based on World Health Or-ganization rehabilitation competency framework(RCF). Methods The competency requirements of TCM rehabilitation in higher TCM institutions were analyzed using RCF the-ory and method,through literature analysis and expert surveys, Results A comprehensive analysis was conducted to extract the practice,professionalism,learning and development,and research competency of TCM rehabilitation professionals,considering the characteristics of the educational envi-ronment in TCM institutions,the training orientation of TCM rehabilitation programs,core knowledge and ethi-cal requirements. Conclusion The adoption of the RCF to explore the competency of TCM rehabilitation professionals helps enhance the professionalism and applicability of the TCM rehabilitation field,providing ideas and methods for cultivating high-quality TCM rehabilitation professionals.

This paper expounds the medical ethic qualities of Qiu Fazu's putting the patients'interests above all else,including the attitude of treating patients with excellent medical skills and benevolent love,the working style of being highly responsible and careful,the never-ending academic spirit of being rigorous and realistic,the medical concept of strict enthusiasm and sincere care,and the moral quality of indifference to fame and wealth and no partiality.It is indicated that family education environment,excellent traditional Chinese culture,moral models leading are the formation logic of Qiu Fazu's medical ethics.Qiu Fazu's views on medical ethics are of great value to the education of medical ethics in the new era.To improve the level and effectiveness of medical ethics education,it is necessary to strengthen the excellent traditional Chinese culture education,strengthen international exchanges and cooperation,lead the advanced moral examples,and cultivate social practice ability.

[摘 要] 宫颈癌是一种发病率和转移率均较高的妇科癌症。尽管手术、放射、化学治疗在宫颈癌治疗中取得了显著的临床效果，但仍存在一定的局限性，因此亟需新的治疗药物。近年研究发现，Wnt/β-catenin 信号通路在宫颈癌发生发展、转移、耐药等过程中起关键性作用。Wnt/β-catenin信号途径参与宫颈癌的发生发展和远处转移及对化疗药物的耐受性；天然化合物如中药白藜芦醇和黄芩素等及合成化合物如伊曲康唑等均可通过此信号通路发挥抗宫颈癌的作用；非编码RNA和一些蛋白分子均可以通过此通路影响宫颈癌的发生发展。研究说明，Wnt/β-catenin信号途径是宫颈癌治疗的潜在靶点。本文归纳宫颈癌中Wnt/β-catenin信号途径的作用，以期为宫颈癌的临床研究与新药开发提供参考依据。

Objective To investigate whether quercetin inhibits pyroptosis of mouse fibroblast NIH-3T3 cells by regulating the NLRP3/caspase-1/GSDMD signaling pathway.Methods NIH-3T3 cells were treated with quercetin or MCC950(a specific inhibitor of NLRP3)before stimulation with lipopolysaccharide(LPS)and ATP to induce cell pyroptosis.The optimal quercetin concentration and duration were screened using the CCK-8assay after testing various concentrations and times.Morphological changes of the treated cells was observed,and the levels of IL-18 and IL-1β in the cell culture supernatant were detected with ELISA;the protein expressions of NLRP3,cleaved caspase-1,and GSDMD-N and the mRNA levels of NLRP3,caspase-1 and GSDMD were detected using Western blotting and qRT-PCR.The changes in cell pyroptosis were examined with TUNEL staining and LDH release assay.Results The CCK-8 assay indicated that 24-hour treatment with 20 μmol/L quercetin yielded the most favorable results.LPS and ATP stimulation of NIH-3T3 cells induced obvious swelling,cell membrane rupture and leakage of cell contents,significantly increased IL-18 and IL-1β levels,and enhanced protein expressions of NLRP3,cleaved caspase-1 and GSDMD-N and mRNA levels of NLRP3,caspase-1 and GSDMD.LPS and ATP stimulation also caused a significant increment of TUNEL-positive cell counts and LDH release in NIH-3T3 cells.Treatment with quercetin or MCC950 significantly reduced cell pyroptosis induced by LPS and ATP,lowered the concentrations of IL-18 and IL-1β,decreased the expression levels of NLRP3,caspase-1/cleaved caspase-1,GSDMD/GSDMD-N,and reduced the number of TUNEL-positive cells and LDH release.Conclusion Quercetin suppresses pyroptosis of mouse fibroblasts stimulated with LPS and ATP and reduces secretion of inflammatory cytokines by inhibiting the NLRP3/caspase-1/GSDMD pathway.

OBJECTIVE To explore the distribution pattern of traditional Chinese medicine(TCM)syndrome types of primary o-varian insufficiency(POI)sleep disorders and the differences in the distribution of sleep quality index among different syndrome types,in order to provide a basis for syndrome differentiation treatment and prevention of POI associated with sleep disorders.METHODS 600 POI patients who met the inclusion criteria were collected for epidemiological investigation,and 405 patients who met the diagnosis of sleep disorders were selected as the research group.The patients'general information,TCM four diagnosis and sex hormone level in-formation were collected,and the Pittsburgh sleep quality index(PSQI)scale was used to evaluate patients'sleep conditions,and ana-lyze the characteristics and influencing factors of TCM syndrome types of POI associated with sleep disorders.RESULTS The main TCM syndrome types of POI accompanied by sleep disorders were heart and kidney disharmony syndrome(41.98%),spleen and kid-ney yang deficiency syndrome(22.22%),kidney deficiency and liver stagnation syndrome(20.99%),and kidney deficiency and blood stasis syndrome(14.81%).The heart and kidney disharmony syndrome had the longest sleep latency and shortest sleep time,relied more on hypnotic drugs,and had the highest PSQI total score;the heart and kidney disharmony syndrome and kidney deficiency and liver stagnation syndrome had the worst sleep quality;the spleen kidney yang deficiency syndrome had the highest daytime dysfunc-tion score.There was no significant difference in FSH levels among different TCM syndrome types;the distribution of E2 values from low to high was:heart and kidney disharmony syndrome,kidney deficiency and liver stagnation syndrome,spleen and kidney yang de-ficiency syndrome,and kidney deficiency and blood stasis syndrome,and there were significant differences among multiple groups(P<0.05).CONCLUSION The main TCM syndrome types of patients with POI and sleep disorders are heart and kidney disharmony syn-drome,spleen and kidney yang deficiency syndrome,kidney deficiency and liver stagnation syndrome,and kidney deficiency and blood stasis syndrome.Among them,the most common TCM syndrome type with the worst sleep quality is heart and kidney disharmony syn-drome,which may be closely related to estrogen E2 levels.

Fritillaria ussuriensis is a valuable traditional Chinese medicine in cold regions,which has the functions of dispersing stagnation and relieving depression,moistening lung and clearing heart.It is widely used in the treatment of diseases such as qi ad-verseness and cough,pharyngitis,sore and carbuncle,etc.Whether it is used alone or in combination,it has good results.It is a pure natural and efficient drug with broad development prospects.In recent years,with the deepening of research on Fritillaria ussuriensis,alkaloids,nucleosides,polysaccharides,volatile oils,organic acids and other components have been isolated.It has pharmacological activities such as relieving asthma,relieving cough,expectorant,calming,anti-inflammatory and antibacterial.By reviewing the reports of chemical constituents and pharmacological effects of Fritillaria ussuriensis in the past 40 years,this paper systematically summarizes and analyzes the research results in order to provide scientific basis for the study of pharmacodynamic substance basis and promote the development and utilization of Fritillaria ussuriensis.