To non-contact alarm the sleep apnea under low physical and mental load condition a device was designed including the modules of minimized bio-radar, signal conditioning, control, alarm and power supply, which can realize the function of non-contat detection of the breathing signal, sleep apnea detection and alarm. Experimental results showed that the device can not only non-contact detection the breathing signal without any sensors or electrodes touching the human body, but also has the advantages of smal volume, low power consumption and low price, which may be widely used in monitor the patients with sleep apnea at home or in the hospital.

Science of cardiac electrophysiology (EP), a most complicated professional branch of car-diovascular medicine, is composed of theoretical knowledge of EP, anatomical knowledge, catheter manipu-lation skills, and antiarrhythmic drug therapy. Longer growth period is involved in clinical cardiac EP physicians due to abstract and complicated electrophysiology knowledge. Starting with simple operation and case to stimulate students' interest in learning, this study focuses on the four teaching modules such as the theory of electrophysiology, the knowledge of anatomy, the skill of the operation of the catheter and the use of antiarrhythmic drugs. It also guides and trains students' simplified habit of thinking and independent thinking and induction learning ability, which improves the cardiac electrophysiologic doctors' efficiency of clinical practice.

How to deliver small interfering RNA(siRNA)molecules into target cells efficiently and safely to silence target genes is a bottleneck in the clinical application of RNA interference. In recent years,siRNA delivery technology based on cell pene-trating peptides has developed rapidly. Among them,the secondary amphipathic peptide,called CADY,has little cytotoxicity and no immunogenicity,forms stable siRNA complexes and improves their delivery into a wide variety of cell lines. Therefore,it has become a very promising delivery carrier for siRNA applications. This review summarizes the design idea,structural characteristic,spatial con-figuration,internalization mechanism,modification and application of CADY.

Objective:To explore mechanisms underlying the rescuing effects of transplanted mesenchymal stem cells (MSCs) derived exosomes on estrogen-deficient osteoporosis.Methods:Mouse estrogen-deficient osteoporosis model was constructed in 12 female C57BL6/J rats and the exosome release was regulated by siRNA.Osteogenic induction,alizarin red staining and qPCR were performed to evaluate the effects of exosomes on recipient MSC functions.The miR-26a mimics and inhibitors and qPCR were used to explore the mechanisms underlying exosome-mediated functional rescue of recipient MSCs.Results:Donor MSCs alleviated estrogen-deficient osteoporosis via exosome release,and the alleviated osteoporosis by exosomes rescued the recipient MSC functions were observed.Moreover,the rescued recipient MSC functions by exosomes transferred miR-26a were found.Conclusion:Donor MSC-derived exosomes may rescue MSC functions and may remit osteoporosis of the recipient through transfering miR-26a.

Objective:To observe the effect of intrathecal administration of 8-O-acetyl-SM(8-OaS) on chronic neuropathic pain in rats by inhibiting the expression of protein kinase B(AKT)-mammalian target of rapamycin(mTOR) signaling pathway in spinal dorsal horn after spinal nerve ligation.Methods:The rat model of neuropathic pain was established by lumbar 5 spinal nerve ligation(SNL), and Von Frey filament was used to investigate the mechanical allodynia. Immunofluorescent histochemistry was adopted to investigate the distribution of pAKT and pmTOR in spinal dorsal horn. The protein levels of pAKT and pmTOR in spinal dorsal horn after drug ad-ministration were quantitatively determined by Western blot. Results: Compared with that in the sham group, the paw withdrawal threshold (PWT) significantly decreased(P<0.01),and the intrathecal administration of 8-OaS attenuated mechanical allodynia ob-viously during the first day and the seventh day after the operation(P<0.05). Meanwhile,double immunofluorescent staining showed the co-expression of pAKT and astrocytes marker glial fibrillary acidic protein(GFAP),and positive labeling of pmTOR was expressed in spinal astrocytes and neurons. The results of Western blot revealed that the protein levels of pAKT and pmTOR in spinal dorsal horn were significantly reduced after the treatment of 8-OaS. Conclusion:Intrathecal administration of 8-OaS attenuates the PWT of SNL-in-duced chronic neuropathic pain. The underlying mechanism of the potential anti-allodynia effect of 8-OaS may be related to the suppres-sion of spinal astrocytes via decreasing the phosphorylation of AKT-mTOR signaling pathway resulting in attenuating the development of neuropathic pain.