1.High-affinity glutamate transporters and chronic pain
Hua ZHANG ; 甘肃省骨关节疾病研究重点实验室 ; Shenghong WANG ; Qiqi XIE ; Wenzhou LI ; Weidong SHI ; Jinglin MA ; Jing WANG
Journal of Central South University(Medical Sciences) 2017;42(9):1094-1099
Glutamate serves as a major excitatory neurotransmitter in the mammalian central nervous system and is stored in synaptic deft by an uptake system that is dependent on the high-affinity glutamate transporters (ETTAs),which locate in the plasma membrane of glial cells and neurons.ETTAs can rapidly terminate the action of glutamate and maintain its normal physiological functions.If the content or function of glutamate transporters is abnormal,it can result in many physiological dysfunctions.Studies have demonstrated that high-affinity glutamate transporters play an important role in the development of chronic pain,which might be a new therapeutic target for the pain.
2.Progress in Wnt signaling pathway and tumorigenesis
Jiexian DING ; Jin ZHANG ; Yonggang CHEN ; Xingwen WANG ; Shuanke WANG
Practical Oncology Journal 2019;33(1):73-77
The Wnt/beta-catenin signaling pathway plays an important role in tumorigenesis and development,and is a key pathway mediating carcinogenesis. Therefore,further studies of Wnt/beta-catenin signaling pathway may provide powerful help for exploring the pathogenesis of tumors and seeking effective diagnosis and therapy. This article reviews the research progress of Wnt/be-ta-catenin signaling pathway and tumorigenesis.
3.Comparative study of open surgery and arthroscopic assisted surgery in management of knee dislocations
Jinwen HE ; Dacheng ZHAO ; Bin GENG ; Yuanjun TENG ; Chengjun ZHANG ; Meng WU ; Xiangdong YUN ; Hua HAN ; Yayi XIA
Chinese Journal of Trauma 2021;37(2):114-121
Objective:To compare the efficacy of open surgery and arthroscopic assisted surgery in treatment of knee dislocations.Methods:A retrospective case-control study was conducted to analyze the clinical data of 80 patients with knee dislocations admitted to Second Hospital of Lanzhou University from May 2013 to September 2019, including 59 males and 21 females, aged 18-66 years [(42.5±11.6)years]. Open multiple ligament reconstruction was performed in 49 patients (open group) and arthroscopic assisted multiple ligament reconstruction was performed in 31 patients (arthroscopic group). The postoperative hospitalization days, incidence of complications, time needed for recovery of knee range of motion (>0°, >90°, >120°), and time to complete weight-bearing were compared between the two groups. The Lysholm score, international knee documentation committee (IKDC) subjective knee form, Tegner activity level, score of the MOS item short-form health survey (SF-36), patient satisfaction and knee range of motion were compared between the two groups at the last follow-up.Results:All the patients were followed up for 1.2-7.4 years [(3.8±1.5)years]. There was no significant difference in postoperative hospitalization days or incidence of complications between the two groups ( P>0.05). No significant difference was found in time needed for recovery of knee range of motion (>0°, >120°) or time to complete weight-bearing ( P>0.05). The time needed for recovery of knee range of motion (>90°) was 90(60, 90)days in open group and 60(30, 90)days in arthroscopic group ( P<0.05). At the last follow-up, there was no significant difference in Lysholm score, IKDC subjective score, Tegner activity level, SF-36 score, or patient satisfaction between the two groups ( P>0.05). At the last follow-up, the knee range of motion was 120°(90°, 130°) in open group and 135°(120°, 140°) in arthroscopic group ( P<0.05). Conclusion:For treatment of knee dislocations, open surgery and arthroscopic assisted surgery have similar results in the long-term effect, while arthroscopic assisted surgery has benefits in early rehabilitation and ultimately better knee range of motion.
4.Endogenous neural stem cells promotes Notch-1 expression after spinal cord injury in rats
Kai WANG ; Na WANG ; Yuliang WANG ; Jingsheng LIU ; Yayi XIA ; Bin ZHAO
Journal of Chinese Physician 2017;19(9):1322-1325,1329
Objective To explore the expressions and changes of endogenous neural stem cells (ENSCs) and Notch-1 after acute spinal cord injury in adult rats,and to explore its role in the nerve regeneration process.Methods The 30 Sprague Dawley (SD) female rats (3-6 months) (300-350 g) were divided into control groups (n =5) and experimental group (n =25) by digital random method.The control group only accepted lamina decompression,and the experimental group was the spinal cord injury group.Five rats were drawn when at 1 d,3 d,1 w,2 w,and 4 w,then behavior,histology,immunohistochemistry and immunofluorescence method were used to detect the proliferation and expression of endogenous neural stem cells and Notch-1 protein.Results Behavioral observation showed the experimental group rats were disfunction.Histological observation showed nerve fiber structure turbulence,edema and denaturation in the experimental group.Immunohistochemistry staining showed the Notch-1 expression every experimental group.Notch-1 positive cell peaked at 3 days.Immunofluorescence test showed,in the experimental group damage to segment the area surrounding the BrdU positive staining cells was significantly higher than the control group.Using three-dimensional quantitative study method detected in 1 w after spinal cord injury was the number of newborn cells mitosis,most about was about 75 times in the control group.Linear regression method was used to analyze the different time points after BrdU and Notch-1 protein expression positive area,the area of the results found that both into linear correlation.Conclusions The new born neurons after spinal cord injury in rats Notch-1 expression has a certain relevance.In addition,the expression of signal protein Notch-1,might be associated with early proliferation of ENSCs in rats.