The basic functions and characteristics of AUTU-IFC, Union Remote Access System, Shangyi Easy Share System and VPN358 were described in this paper in order to help the users to select their appropriate remote access systems and meet their requirements to access digital resources at any time and at any place.

c-MET is considered a promising oncogenic driver in non-small cell lung cancer (NSCLC) after the discovery of epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK).MET activation including gene mutation,amplification and protein overexpression,all of these are potential therapeutic targets and are associated with poor prognosis.Clinical evidence suggests a role for MET activation as both a primary oncogenic driver in subsets of lung cancer,and as a secondary driver of acquired resistance to EGFR-tyrosine kinase inhibitor (TKI).This review focuses on the MET activation in NSCLC and the latest trials of its treatment.

Based on our research team's clinical and basic research results of traditional Chinese medicine (TCM) in the prevention and treatment of ischemic stroke, and combined with TCM syndrome standard manifestations of this disease, wind, phlegm, fire, deficiency and blood stasis, this paper points out definitely that the basic pathogenesis of ischemic stroke is blood stasis caused by deficiency of both Qi and Yin. The pathogenic characteristics are deficiency in origin and excess in superficiality, and the wind, fire, phlegm, deficiency and blood stasis are related one another resulting in this disease; it is established that the basic syndrome types of this disease are deficiency of both Qi and Yin and static blood blocking the collaterals, and the therapeutic regime is a recipe including the TCM ingredients having the actions of nourishing yin, tonifying Qi and improving blood circulation (Yangyin Yiqi Huoxue) and according to the patient's individual variation of syndrome differentiation, the ingredients in the recipe can be added or subtracted. In this report, a new system of treatment for ischemic stroke based on syndrome differentiation is proposed.

Taiyi moxa-stick moxibustion refers to the method of compressing the ignited moxa-stick which is made of medicine and moxa on the acupoints by the partition of paper with Taoism magic figures,and the method has achieved significant efficacy in clinic.Taiyi moxa-stick moxibustion was first introduced to Lingnan area by FAN Yu-Ni in the Qing Dynasty when he was an official in Guangdong,and has been widely spread since then.In the book of Jing Yan Liang Fang(Empirical Prescriptions)which was compiled by ZHOU Gui-Shan born in Shunde region and in Qing Dynasty and was popular in Lingnan area,there is a unique method of ginger-separated Taiyi moxa-stick moxibustion,which appeared earlier than the ginger-separated and bowl-covered Taiyi moxa-stick moxibustion created by YE Gui in 1856.The comparison of 19 kinds of works on Taiyi moxa-stick moxibustion issued in the Qing Dynasty showed that the unique ginger-separated Taiyi moxa-stick moxibustion was only recorded in several Lingnan medical books,the medicated prescription for Taiyi moxa-stick moxibustion(composed of 12 drugs,namely Moschus,Aconiti Radix,Aconiti Kusnezoffii Radix,Arisaematis Rhizoma,Pinelliae Rhizoma,Realgar,Orpiment,Caryophylli Flos,Asari Radix et Rhizoma,Clematidis Radix et Rhizoma,Scolopendra and Sulfur)was without moxa added,and the drugs were made into the medicine block for ginger-separated moxibustion.In this article,the origin and characteristics of Lingnan ginger-separated Taiyi moxa-stick moxibustion were investigated to provide ideas for the expansion of clinical application of moxibustion.

OBJECTIVE To study the toxicological effect of matrine (MT) on PC12 cells and the mechanism of mitochondrial damage. METHODS After treatment with MT 2, 4 and 8 mmol·L-1for 8, 16,24 and 48 h,respectively,the viability of PC12 cells was measured with methylthiazolyltetrazolium assay.PC12 cells were treated with MT 2,4 and 8 mmol·L-1for 24 h,before the morphological changes were observed by Hoechst33342 staining. The superoxide dismutase (SOD) activity and malondialde-hyde (MDA) content in cells were detected using hydroxylamine method and thiobarbituric acid method, apoptosis rate and reactive oxygen species (ROS) of PC12 cells were detected with flow cytometry, mitochondrial membrane potential (MMP) was detected with JC-1 staining, and the expressions of procaspase 3, procaspase 9, cleaved-caspase 3, Bax and Bcl-2 were detected with Western blotting. RESULTS MT inhibited the growth of PC12 cells in a time-and concentration-dependent manner.After being treated with MT for 24 h,the nuclei of PC12 cells in MT groups showed chromatin agglutination and partial rupture to different degrees,and compared with normal control group,the apoptosis rates of MT 2,4 and 8 mmol·L-1groups were significantly increased(P<0.01).Intracellular ROS and MDA levels increased (P<0.05, P<0.01), SOD activity decreased (P<0.01), and MMP decreased. The expressions of procaspase 9, procaspase 3 and Bcl-2 were significantly decreased (P<0.01, P<0.05), the expres-sions of cleaved-caspase 3 and Bax were significantly increased(P<0.05,P<0.01),and the ratio of Bcl-2/Bax was significantly decreased (P<0.01). CONCLUSION MT has toxic effect on PC12 cells and induces apoptosis through ROS mediated mitochondrial pathway.

Objective:To obtain the optimum extraction conditions for quercetin and kaempferol from Lindera aggregate leaves. Methods:On the basis of single factor investigation, Box-Behnken experimental design was used to provide the experimental data for establishing a regression model for the extraction of quercetin and kaempferol. Response surface and contour diagrams with the extrac-tion yield of quercetin and kaempferol as the response values,were plotted for analyzing the pairwise interactive effects of hydrochloric acid concentration, hydrolysis temperature and hydrolysis time. Results:The hydrochloric acid concentration of 4. 1%, the hydrolysis temperature of 83℃ and the hydrolysis time of 45min were the optimum extraction conditions. With the above conditions, the content of quercetin and kaempferol from Lindera aggregate leaves was 6. 97 mg·g-1 and 2. 82 mg·g-1 , respectively. The Lindera aggregate beaves from five different habitals were analyzed,and the total content of quercetin and kaempferide from Taizhou Tiantai were highest. Conclusion:The quercetin and kaempferol extraction from Lindera aggregate leaves is optimized by Box-Behnken response surface method and the process is convenient and feasible.

Objective:To obtain the optimum extraction conditions for quercetin and kaempferol from Lindera aggregate leaves. Methods:On the basis of single factor investigation, Box-Behnken experimental design was used to provide the experimental data for establishing a regression model for the extraction of quercetin and kaempferol. Response surface and contour diagrams with the extrac-tion yield of quercetin and kaempferol as the response values,were plotted for analyzing the pairwise interactive effects of hydrochloric acid concentration, hydrolysis temperature and hydrolysis time. Results:The hydrochloric acid concentration of 4. 1%, the hydrolysis temperature of 83℃ and the hydrolysis time of 45min were the optimum extraction conditions. With the above conditions, the content of quercetin and kaempferol from Lindera aggregate leaves was 6. 97 mg·g-1 and 2. 82 mg·g-1 , respectively. The Lindera aggregate beaves from five different habitals were analyzed,and the total content of quercetin and kaempferide from Taizhou Tiantai were highest. Conclusion:The quercetin and kaempferol extraction from Lindera aggregate leaves is optimized by Box-Behnken response surface method and the process is convenient and feasible.

Objective To analyze the medication status of patients after in vitro fertilization-embryo transfer(IVF-ET),in order to promote clinical rational medication and lay the foundation for developing new pharmaceutical service models.Methods The prescriptions of western medicines and traditional Chinese patent medicines of 513 patients after IVF-ET who came to Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University from January to December 2022 were selected and analyzed.Results A total of 1377 outpatient prescriptions were randomly checked,and the most commonly diagnosed of the prescriptions were threatened abortion(47.36％).Other diagnoses included thrombophilia,leukorrheal disease,and autoimmune disease,etc.The most frequently used drugs were antiabortifacient drugs(38.51％),followed by anticoagulants(16.96％)and assisted reproductive drugs(16.87％).The study found that the prescription medication situation was basically reasonable,and unreasonable prescriptions were mainly due to unsuitable indications(2.83％).Conclusion The medication used by patients after IVF-ET in outpatient department of our hospital is basically reasonable.It is recommended to strengthen the training of clinical doctors and pharmacists on rational medication knowledge,and carried out a variety of new models of patient-centered pharmacy services.

Objective To understand the community transitional care in the process of development of the difficulties and problems. Methods The qualitative research method was used to conduct semi-structured interviews with community nurses and community care managers(11 cases) who met the standards and analyzed the interview data with Colaizzi 7 footwork. Results The factors that affected the effective care of the community were:there was no clear continuation of nursing service fees, lack of internal and external incentive mechanism; home service risk, no security; community human resources, job responsibilities were not clear; the mechanism was not perfect, the lack of medical cooperation with large hospitals;community nurses professional ability uneven;patients did not trust the community health service agencies, community nurses were not taken seriously. Conclusions There are many obstacles in the community to carry out continuous care, the establishment of hospital-community-family linkage type of continuous care to the government and the Ministry of Health within the practical issues for the development of effective and effective care service system and policy.

Objective To investigate the status of cognitive function and leisure activity participation among the elderly in urban communities, and to explore the relationship between cognitive activities and leisure activities. Methods Using the General Demographic Data Questionnaire,the Montreal Cognitive Assessment Scale and Leisure Activities Questionnaire, 528 elderly people were investigated. Results The cognitive function were (25.65 ± 2.58), (25.76 ± 2.48)points in elderly with recreation and intelligence activities respectively, and (25.21 ± 2.53), (25.58 ± 2.60)points in elderly without recreation and intelligence activities respectively, the difference was statistically significant ( Z=5.56, 8.16, P<0.01). There was no significant difference between elderly with physical activity and social activities and without physical activity and social activities (P>0.05). The participation of intelligence and recreation was positively correlated with cognitive function (r =0.347, 0.270, P < 0.01), while social activities and physical activities were not correlated with cognitive function (P>0.05). Conclusions Nursing staff should pay more attention to the decline of cognitive function of the elderly, so that the elderly can recognize the importance of leisure activity, improve their enthusiasm, participate in science, enrich the quality of life in later years, improve cognitive function.