Objective To investigate the distribution and drug susceptibility of vaginal pathogens in pregnant women during perinatal period.Methods Vaginal discharge specimens of 10 800 women (5 400 were pregnant women during perinatal period,5 400 were non-pregnant women) were performed bacterial culture and drug susceptibility testing.Results The isolation rate of pathogens from 5 400 perinatal pregnant women was 26.00％ (n =1 404),including 759 strains of fungi,611 strains of gram positive cocci,32 strains of gram-negative bacilli,2 strains of Neisseria gonorrhoeae;among 5 400 non-pregnant women,the isolation rate of pathogens was 7.87％ (n=425),including 232 strains of fungi,182 strains of gram-positive cocci,5 strains of gram negative bacilli,and 6 strains of Neisseria gonorrhoeae.Resistance rates of Streptococcus agalactiae isolated from perinatal pregnant women to erythromycin and clindamycin were 84.85％ and 80.81 ％ respectively,resistance rate of Staphylococcus aureus to erythromycin was 40.91 ％,resistance rates of Escherichia coli to tetracycline and sulfamethoxazole/trimethoprim were 69.23 ％ and 53.85 ％ respectively,resistance rate of Neisseria gonorrhoeae to penicillin and sulfamethoxazole/trimethoprim was 100.00％.Conclusion Vaginal infection rate in perinatal pregnant women is higher than non-pregnant women,screening of vaginal pathogens in perinatal pregnant women should be strengthened,so as to ensure the safety of mothers and infants.

Objective To observe the clinical effect of aerosol inhalation of recombinant human α2b injection for children with viral pneumonia. Methods 50 children with viral pneumonia admitted to our hospital from November 2016 to November 2017 in our hospital were selected and divided into control group and observation group according to different inhalation drugs. The control group was given ribavirin aerosol inhalation therapy and the observation group was treated with nebulization of recombinant human α2b injection. The clinical efficacy and changes of serological inflammatory indicators were compared between the two groups. Results The good rate of observation group was 96. 00％ (24/25) and was significantly higher than that of control group which was 80. 00％ (20/25)(P＜0. 05). Blood routine examination, erythrocyte sedimentation rate, C-reactive protein, IL-6 (interleukin-6) and IL-10 (interleukin-10) of serum inflammatory indexes in observation group were all significantly lower than those in the control group (P＜0. 05). Conclusion The inhalation of recombinant α2b injection for the treatment of viral pneumonia in children has a definite clinical effect. It has a high good rate of clinical treatment and has a definite curative effect on the reduction of serological inflammatory indicators which is worth promoting.

Objective To study the clinical application value of serum albumin and unconjugated bilirubin detection in neonatal jaundice. Methods A total of 86 cases of neonatal jaundice were enrolled in this study. The serum albumin was measured by ELISA analysis. The unconjugated bilirubin was measured by vanadate oxidation analysis. Results There were significant differences of serum albumin, unconjugated bilirubin, and total bilirubin between moderate jaun-dice group and severe jaundice group(P<0.05﹚. The expressions of serum albumin, unconjugated bilirubin, and total biliru-bin were significant differences among infection, hemolytic, and obstructive jaundice group (P<0.05﹚. Conclusion The detection of serum albumin,unconjugated bilirubin and and total bilirubin has something to contribute to diagnose, exti-mate pathogenetic condition, and analyze etiological factor in neonatal jaundice.

Objective To evaluate the distribution and antimicrobial resistance of pathogens in neonates.Methods Blood culture specimens from hospitalized neonates in the neonatal intensive care unit (NICU) of a maternal and child heath hospital between January 1,2013 and December 31,2015 were investigated retrospectively,blood culture and antimicrobial susceptibility testing results were analyzed with WHONET 5.6 software.Results A total of 255 strains were isolated from blood culture specimens,including 219 strains(85.88 ％) of gram-negative bacteria,29 strains(11.37％) of gram-positive bacteria,and 7 strains(2.75％) of fungi.The isolated bacteria were mainly Klebsiella pneumoniae (n =157),Escherichia coli (n =35),and Streptococcus B (n =13).Resistance of Escherichia coli to ampicillin and piperacillin were 97.14％ and 94.29％ respectively,all were sensitive to cefoxitin,imipenem,meropenem,ertapenem,and amikacin.Resistance rates of Klebsiella pneumoniae to piperacillin,cefazolin,and cefuroxime were 93.63％,87.90％,and 85.35 ％ respectively,but was sensitive to amikacin.Resistance rates of the main gram-negative bacteria to enzyme inhibitor (amoxicillin/clavulanic acid,ticarcillin/clavulanic acid),cephalosporins (ceftazidime,cefoxitin),and sulfonamides (sulfamethoxazole compound) increased,difference were all statistically significant (all P＜0.01).Conclusion Klebsiella pneumoniae and Escherichia coli are the major pathogens causing bloodstream infection in NICU,and resistance rates to a multiple antimicrobial agents are high,empirical medication for anti infection treatment should be combined with local data.

Objective To investigate the internal and external factors of scientific and technological innovations in pro-vincial maternal and child health care institutions and propose effective strategies for facilitating the innovations.Methods The PEST-SWOT model was used to analyze the internal strengths and weaknesses,external opportunities,and threats of the scientific and technological innovations in a provincial maternal and child health care institution from the perspectives of political,econom-ic,social,and technical environment.Results The institution has advantages and opportunities in policy support,scientific re-search management and investment,and disciplinary characteristics.However,it faces some threats and has disadvantages in tal-ent team,information-oriented level,and industrial competition.Conclusion The development of scientific and technological in-novation in provincial maternal and child health care institutions is affected by internal and external factors.It is necessary to firmly seize opportunities and comprehensively promote scientific and technological innovations from the aspects of medical treat-ment,teaching and research collaboration,discipline layout,talent team,and information-oriented construction.

Objective To investigate the changes in ovarian function and the radiotheraputic influence on ovarian function on patients with cervical squamous cell carcinoma.Methods We retrospectively analyzed 53 cases of cervical cancer patients FIGO staging Ⅰ B1-Ⅱ B who had received ovarian transposition surgery at the Maternal and Child Health Hospital of Jiangxi province from January 2009 to June 2012.All the patients included in the study were FIGO staging Ⅰ B1-Ⅱ B and had undergone radiation therapy,including 38 staging Ⅰ B1-Ⅱ A2 cervical cancer patients receiving chemo-therapy after radical radiotherapy due to the presence of risk factors and other 15 patients with stage Ⅱ B to radical concurrent chemoradiotherapy ovarian transposition.Ovarian transposition methods would include laparoscopic ovarian transposition and transabdominal ovarian transposition.15 concurrent patients with stage Ⅱ B who currently receiving chemo-radiotherapy were under laparoscopic ovarian transposition.Among the 38 radical hysterectomy patients,31 were having abdominal ovarian transposition,and the remaining 7 cases were laparoscopic.All the 53 patients had undergone radiotherapy.The levels of serum female hormones FSH,LH,E2 were determined to monitor the ovarian endocrine function.Results According to FIGO staging,18 cases were stage Ⅰ B 1,15 cases Ⅰ B2,3 cases Ⅱ A1,2 cases Ⅱ A2 and 15 cases Ⅱ B.Patients' age range was from 28 to 44 years old,with an average of 37.7 years,median age as 38 years.14 patients (63.6％) were still normal ovarian function after radiotherapy by laparoscopic ovarian transposition,which was 100.0％before radiotherapy.There was a significant difference (P＜0.05) compared with before radiotherapy.After transabdominal ovarian transposition surgery and radiotherapy,normal ovarian function 22 cases (71.0％),and there was a significant difference (P＜0.05) compared with before radiotherapy.No significant difference was found with regard to the proportion of normal ovarian function after radiotherapy between the two groups of patients with laparoscopic and transabdominal ovarian transposition (P＞0.05).Conclusion For the young cervical cancer patients,even with ovarian transposition,ovarian dysfunction was still evident after radiotherapy.There was no significant difference between laparoscopic and transabdominal ovarian transposition.

Objective:To compare the clinical effect of dydrogesterone and progesterone in the treatment of threatened abortion.Methods:From March 2015 to May 2017, 50 patients with threatened abortion admitted in the Maternal and Child Health Hospital of Ninghai County were selected and randomly divided into two groups according to the digital table, with 25 cases in each group.The control group was treated with progesterone.The observation group was treated with dydrogesterone.The hormone levels, symptom disappearance time, fetal retention and adverse drug reactions were compared between the two groups.Results:One week after treatment, the levels of progesterone, estradiol and human chorionic gonadotropin in the observation group were (1 12.65±6.35)nmol/L, (2 137.99±371.22)pmol/L, (4 261.54±245.55)IU/L, respectively, which in the control group were (101.56±5.32)nmol/L, (1 782.12±350.71)pmol/L, (3 642.26±210.20)IU/L, respectively, the differences between the two groups were statistically significant ( t=6.694, 3.484, 9.581, all P<0.05). Three weeks after treatment, the levels of progesterone, estradiol and human chorionic gonadotropin in the observation group were (120.52±10.59)nmol/L, (2 240.68±409.06)pmol/L, (102.64±9.64)nmol/L, and (6 641.52±364.59)IU/L, respectively, which in the control group were (102.64±9.64)nmol/L, (1 921.76±371.07)pmol/L, (4 123.63±258.62)IU/L, respectively, the differences between the two groups were statistically significant( t=6.243, 2.887, 28.164, all P<0.05). Five weeks after treatment, the levels of progesterone, estradiol and human chorionic gonadotropin in the observation group were (134.15±17.16)nmol/L, (2 317.58±434.97)pmol/L, and (8 456.51±459.52)IU/L, respectively, which in the control group were (109.56±8.54)nmol/L, (2 051.38±413.39)pmol/L, (6 416.36±261.31)IU/L, respectively, the differences between the two groups were statisticallysignificant ( t=6.414, 2.218, 19.297, all P<0.05). The disappearance time of pain in the waist, abdominal pain and vaginal bleeding in the observation group was (4.15±0.25)d, (3.10±0.52)d, (4.24±0.62)d, respectively, which was shorter than that in the control group[(7.54±2.26)d, (4.31±1.10 d), (6.32±2.01)d]( t=7.455, 4.972, 4.944, all P<0.01). The success rate of fetal preservation in the observation group was 96.00%(24/25), which was higher than that in the control group[72.00%(18/25)](χ 2=5.357, P<0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups( P>0.05). Conclusion:The effect of dydrogesterone in the treatment of threatened abortion is better than progesterone.

Objective To study the therapeutic effect of pulmicort respules combined with terbutaline inhalation on pe-diatric asthmatic bronchial pneumonia. Methods 50 children with asthmatic bronchial pneumonia who were admitted in our hospital from February 2016 to February 2017 were selected as observation objects. The children were randomly divided into two groups including control group and observation group, with 25 cases in each group. The control group was treated with routine treatment. The observation group was treated with pulmicort combined with terbutaline inhalation. The clinical efficacy between the two groups of children was compared. Results The subsiding time of signs and symptoms, and length of hospital stay in the observation group were shorter than those in the control group. The difference was statistically significant (P＜0. 05). The treatment effective rate in the observation group was 96. 0％, higher than that in the control group(72. 0％). The difference of the data was statistically significantP＜0. 05). After treatment, the improvement of the lung function index in the observation group was better than that in the control group, and the differ- ence was statistically significant (P＜0. 05). Conclusion The use of pulmicort combined with terbutaline inhalation has a good effect in the treatment of children with asthmatic bronchial pneumonia, which can quickly eliminate symptoms in children, and shorten the hospitalization time of children, improve children's lung function, and it is worthy of clinical use.

Objective To investigate the validity, timeliness and security of epidural preset tube to block multipara la-bor analgesia, and to improve maternal labor analgesia rates and quality. Methods A total of 270 cases of mature ma-ternal in our hospital from May 2013 to October 2014 were chosen for the study, according to the study requirements, and they were divided into three groups. The observation group(group A) received PCEA labor analgesia method, and the control group1 (group B) received continuous epidural labor analgesia method, and the control group 2 (group C) did not undergo analgesia. Maternal vital signs and fetal heart rate in the three groups were observed, and the first stage of labor, the second stage of labor and the third stage of labor, pain visual analog scale (VAS score), Bromage score, mode of delivery, oxytocin usage rate, newborn Apgar score, maternal hemorrhage amount and the incidence of perineal lac-eration were recorded. Results There were not statistically significant differences in comparing general information of the 3 groups such as age, height, weight, gestational age(P>0.05). The differences in stage of labor and the incidence of perineal laceration in the three groups were statistically significant (P<0.05). The differences were not statistically significant in comparing oxytocin usage rate, cesarean section rate, neonatal asphyxia score, postpartum hemorrhage amount of the observation group and the control group 1 (P>0.05). The differences were statistically significant in com-paring oxytocin usage rate, cesarean section rate, neonatal asphyxia score and postpartum hemorrhage amount in the observation group and the control group 2 (P<0.05). Conclusion Continuous epidural preset tube method to block mater-nal labor analgesia is safe and effective, can improve the timeliness and effectiveness of obstetric complications anes-thesia process, reduce the incidence of perineal laceration, and increase maternity delivery security.

Purpose To investigate the expressions of PTP1B in epithelial ovarian cancer tissues and to analysis its correlation with clinical pathological and survival significance.Methods The expression of PTP1B in ovarian cancer tissues was detected by Oncomine database and immunohistochemistry.The relationships between PTP1B expression and clinicopathological features, including prognosis significance, were analyzed. Results Oncomine database showed that the mRNA expression of PTP1B in ovarian cancer was significantly higher than that in normal tissues (P<0.001). Immunohistochemical staining showed that PTP1B expression was significantly correlation with FIGO stage (P<0.001), omentum majus metastasis (P=0.002). Kaplan-Meier analyses revealed that ovarian cancer patients with high PTP1B expression tumors had a significantly worse overall survival rate than those with low PTP1B expression tumors (P<0.001). Multivariate analysis showed that FIGO stage, PTP1B expression were independent survival predictors.Conclusion PTP1B expression may be involved in the tumor progression and poor prognosis of patients with ovarian cancer, and it might be used as one of the valuable markers for poor prognosis in patients with ovarian cancer.