Objective: To observe the effects of ginsenoside Rg_(1) , chitosan and TGF-?_(1) on the proliferation and differentiation of human periodontal ligament cells( PDLC). Methods:Human periodontal ligament cells were isolated and cultured. The effects of ginsenoside Rg_(1)(0.01 ?mol/L), chitosan((0.05 g/L,)0.1 g/L, 0.2 g/L) and TGF-?_(1)(0.5 ?g/L, 1 ?g/L, 2 ?g/L) on the proliferative ability of human PDLCs were evaluated with MTT method. The alkaline phosphatase activities of human PDLCs were measured with spectrophotometric assay. The secretion of osteocalcin of human PDLCs were measured with radioimmunological method and the apotosis rates of human PDLCs were assayed with flow cytometry with PI staining method. Results: ①Comparing with the control group, the proliferative ability of human PDLCs in ginsenoside Rg_(1)(0.01 ?mol/L)group ,Chi(0.05 g/L, 0.1 g/L, 0.2 g/L) groups and TGF-?_(1 )((0.5 ?g/L), 1 ?g/L, 2 ?g/L) groups on day 3,5,7 were considerably increased (P

Objective To explore the effect of fluticasone propionate nasal spray(Fu Shu-liang) in improving children's symptoms of allergic rhinitis and recurrent asthma.Methods 100 children with allergic rhinitis and asthma syndrome were randomly divided into control group and experimental group.The control group was treated with loratadine.The experimental group was treated with water-soluble auxiliary Fu Shu-liang nasal spay on the basis of the control group.The treatment effects were observed.Results The total effective rate of the observation group was 94％,which was significantly higher than 76％ of the control group (x2 =6.35,P ＜ 0.05).After 10-12 weeks,the rhinitis symptom score and asthma symptom score between the two groups had statistically significant differences (t =2.47,2.64,3.41 ; 2.30,3.17,2.47,all P ＜ 0.05).The incidence rate of adverse reactions such as nasal dryness,epistaxis between the two groups had statistically significant differences (x2 =7.11,7.53,all P ＜ 0.05).Conclusion Fu Shu-liang(Eph propionate and fluticasone propionate nasal spray) has good effect in improving symptoms of children with allergic rhinitis and asthma,it is worthy of clinical vigorously promoted.

Objective To explore the difference of serum and pleural effusion CEA ,CA125 and CA199 levels in benign and ma‐lignant pleural effusions to accumulate the practical experience for clinical diagnosis .Methods 125 patients with pleural effusion in this hospital from February 2010 to March 2014 were performed the retrospective analysis and divided into the benign group(49 ca‐ses) and malignant group(76 cases) according to the nature of the fluids .The etiology composition ,pleural effusion and serum tumor markers levels were recorded in the two groups and at the same time the diagnosis efficiency of the tumor markers in pleural effusion was calculated .Results The specificity of the combined detection of CEA ,CA125 and CA199 for diagnosing benign and malignant pleural effusion was 90 .6% ,the sensitivity was 95 .1% ,the positive predictive value was 90 .2% ,negative predictive val‐ue was 95 .3% and accuracy was 92 .7% ,which were higher than those of the single index detection and any 2‐marker combined de‐tection .Conclusion The combined diagnosis of CEA ,CA125 and CA199 in pleural effusion conduces to the assisted diagnosis of be‐nign and malignant pleural effusion .

Objective To analyze the pathophysiological variation in patients with penetrating cardiac trauma (PCT) and discuss the classified treatment strategies.Methods A retrospective analysis was conducted on pathogenetic condition,medical interventions and other clinical information of 133 PCT patients.The patients were divided into four groups in accordance with their symptom classification on arrival at emergency department,including sub-clinic type,cardiac tamponade type,hemorrhagic shock type and agonal type.Meanwhile,the patients were classified into three groups according to their pathogenesis phase,including sub-clinical phase,clinical phase and agonal phase.Development characteristic of patients' condition in each group was investigated in each time point by survival analysis.Impacts of medical interventions on prognosis of those patients were analyzed,such as pericardial space exploration,emergency room thoracotomy (ERT),operating room thoracotomy (ORT).Results Some patients at sub-clinical phase were aggravated into clinical phase or agonal phase,as well as some patients at clinical phase were aggravated into agonal phase during in-hospital treatment.There were significance differences of posttraumatic suvival course among the four groups,namely sub-clinical type,cardiac tamponade type,hemorrhagic shock type and agonal type (P ＜ 0.01).The differences of posttraumatic survival course were also significant among the three groups,namely sub-clinical phase,clinical phase and agonal phase (P ＜ 0.01).Conclusion Clinical symptom classification (or pathogenesis phase) of PCT may not be always unchangeable,thus it is recommended that PCT patients should be treated based on their clinical symptom classification or patbogenesis phase at consultation.

Ehrlich's ascites cancer (EAC) in BALB/C mice by ip transplantable EAC cell is reproduced by us. The change of serum malondialdehyde(MDA), plasma cellular cAMP and adenylate cyclase (AC) are examined in the early, middle and late phase of mice bearing EAC respectively in comparison with that of control group. Results indicate that serum MDA in mice bearing EAC is continuously increased with the progress of the disease in the remarked ways in comparison with the control group. Whereas the plasma cAMP in the mice bearing EAC is markedly decreased in that order. Relation between the change of serum MDA and the cellular cAMP, AC or plasma cAMP shows a significant negative correlation efficiency (P

Nasopharyngeal carcinoma (NPC) in BALB/c nude mice (NM) was re-produced by hypodermic implanting human CNE-2 cell to the right-side of neck-backposi-tion. The changes of plasma malondialdehyde (MDA) and cAMP/cGMP ratio in NM bea-ring human NPC were examined. Meanwhile, we observed that tumor's size, plasma MDAand cAMP/cGMP ratio in NM bearing human NPC were affected by polysacchoridopetide(PSP). Results indicated that in comparison with the control group, NM bearing humanNPC showed a marked increase in plasma MDA(18.25?1.37 nmol/ml),cAMP/cGMP ratio(4.55?0.35), a marked decrease in plasma, cAMP (71.83?8.51 pM/ml) and cGMP(15.77?0.65 pM/ml), both high or low-concentration PSP inhibited NPC growth.Rate of inhibition was between 59.58 to 95.53 percent. PSP decreased plasma MDA,elevated cAMP, cGMP's levels and recovered cAMP/cGMP ratio. Our results suggestedthat the elevated level of MDA in NM bearing human NPC was closely associated withthe presence of NPC. PSP had antitumor effect, probably by means of decreasing freeradicals and recovering cAMP/cGMP ratio.

Objective To learn the burnout and turnover intention of physicians from township hospitals in Xi County,Henan province,for reference of health administrators and healthcare institution managers.Methods Cluster sampling method and qualitative interviews were used and all physicians from such hospitals were sampled for questionnaire survey in 2014,with 1 70 scales distributed.The questionnaire was the Markov Burnout Inventory generic version revised by Li Chaoping,and turnover intention questionnaire was that revised by Liang Kaiguang.Results 140 physicians from such hospitals were found with zero or low burnout (94.6%);Collaborative care in the county poses great positive effect on promoting professionalism and interpersonal relations, yet has limited positive impact on reducing workload and performance appraisal stress and increasing their income.Conclusion It is imperative to enhance training of managers of such hospitals for them to learn the appraisal method with organizational scale,and build a humanistic concern mechanism for physicians,for purposes of improving the organizational environment and minimize the burnout and turnover intention of the physicians.

Objective To investigate the status of TCM development in Chongqing city,and to put forward countermeasures and suggestions of the development of Chinese medicine in view of the existing problems,so as to provide reference for the government and relevant departments.Methods The relevant questionnaires were issued and recycled in 20 districts and counties of Chongqing city.Part of the data culled from Chinese management office yearbooks of Chongqing.Finally,comprehensive analysis was per-formed.Results There′s enormous upside potential for the development of Chongqing traditional Chinese medicine (TCM),but there were some problems and deficiencies.The main problems were as follows:the TCM characteristics not brought into full play in traditional Chinese Medicine hospitals,lack of following talents,unsound organization management of traditional Chinese medi-cine,the government′s insufficient investment and attention for traditional Chinese medicine (hospitals,schools,“hand-to-hand”ap-proach),not enough national policy support for TCM,TCM hospitals and folk medicines of ethnic minorities,and insufficient exca-vation in ethnic minority medicine.Conclusion In order to adapt to the development of Chinese medicine and to meet the growing requirements of Chinese medicine,some countermeasures should be taken as follows:giving full play to the characteristics of tradi-tional Chinese medicine,strengthening personnel training in Chinese medicine,reforming the management mechanism,increasing the government investment,strengthening policy support for Chinese medicine and folk medicines of ethnic minorities,accelerating the process of TCM standardization,scientific and internationalization.It is suggested that the miscellaneous diseases &incurable dis-ease medicine research office and slow disease traditional Chinese medicine health care office should be established under the TCM management office.Specialized agencies are advised to mine and collect herbal remedies,recipes in ethnic minority area and so on.

Objective To investigate the changes of cervical vagal efferent discharge induced by lipopolysaccaride in rats. Methods Ten rats were randomly divided into two groups, receiving lipopolysaccaride (5 mg/kg) or normal saline through intravenous injection. Frequency of cervical vagal efferent discharge was recorded at 30 min, 1, 2 and 4 h after the injection. Results The frequency of cervical vagal efferent discharge was significantly increased after LPS injection (P

Objective To evaluate the preoperative localization value of MRI and MRCP for congenital biliary dilatation.Methods 30 cases of congenital biliary dilatition diagnosed by either surgery or pathology were studied.MRI and MRCP findings of all these cases were analyzed.Results MRI and MRCP preoperative examination manifested the place of choledochal cyst and the relation between choledochal cyst and the biliary ductal system around in all 30 patients.Through surgery findings as follow,using Todani's classification,the accuracy of MRI and MRCP in evaluating the site and classification of congenital biliary dilatation was 100%,the accurate visualization rate of pancreatic duct was 73.3% and the junction of pancreaticobiliary duct was 33.3%.Conclusion MRI and MRCP manifestations are very reliable in preoperative localization of congenital biliary dilatation.