No abstract available.
Heart Defects, Congenital ; Umbilical Veins*

No abstract available.
Endothelial Cells* ; Humans* ; Umbilical Veins*

A death of one fetus in twin pregnancy is a rare obstetric complication. And the stenosis of umbilical cord artery is a very rare complication of cord abnormalities. The umbilical cord showed a false knot due to accentuation of a vascular spiral with a dilated vein and two arteries with incomplete patency of the lumen. This is the first report of single demise of twin pregnancy due to umbilical artery stenosis and umbilical vein varix.
Arteries ; Constriction, Pathologic ; Fetus ; Humans ; Pregnancy, Twin ; Twins ; Umbilical Arteries ; Umbilical Cord ; Umbilical Veins ; Veins

No abstract available.
Endothelial Cells* ; Hantaan virus* ; Humans* ; Umbilical Veins*

BACKGROUND/AIMS: The fetus liver was characterized by its relatively larger left lobe than right lobe. So far there are no available morphometrical data and shape of the late-stage of human fetal liver, including identification of the intrahepatic vessels, which is little bit different from adult liver. METHODS: Among usual anatomic cadavers in department of anatomy of Sapporo medical university we choose normal- looking 12 late-stage human and 10 adult livers. At first, we measured the thickness and height and width of the livers at each designated sites and than underwent dissection for measurement of major intrahepatic vessels. In fetus, the upward protrusion of S8 was not evident, while S4 provided the greatest thickness of the liver. The fetus revealed an ellipsoid or oval shaped visceral surface and large S3, while the adult liver was triangular. The Arantius duct was almost always narrower than each of the 3 major hepatic veins, and it was often narrower than the umbilical vein. CONCLUSION: Both S2 and S6 seemed to enlarge during the postnatal growth, although there seemed to be great individual variations in the process of the growth. In the late stage fetus, three major hepatic veins seemed to play a great role for the venous return to the heart from the liver, rather then the Arantius duct.
Adult ; Cadaver ; Fetus ; Heart ; Hepatic Veins ; Humans* ; Liver* ; Umbilical Veins

An umbilical vein aneurysm is rare, but appears to be associated with fetal morbidity and mortality. There are no specific guidelines for pregnancy with umbilical vein aneurysm and the management is substantially up to the clinician. We report a case of intra-amniotic umbilical vein aneurysm diagnosed at 35 gestational weeks by ultrasound. Because the aneurysm was growing rapidly, prompt cesarean delivery was conducted. After delivery, a huge fusiform umbilical cord was noted, which was confirmed to be umbilical vein aneurysm by pathological examination. We also reviewed previous reported cases and summarized the management strategies of prenatally detected umbilical vein aneurysms. In addition, the umbilical vein in this case report had the largest size ever reported.
Aneurysm* ; Karyotyping ; Mortality ; Pregnancy ; Ultrasonography ; Umbilical Cord ; Umbilical Veins* ; Varicose Veins

No abstract available.
Angiotensin II ; Angiotensins ; Endothelial Cells ; Endothelins ; Epoprostenol ; Thrombin ; Umbilical Veins

OBJECTIVETo study the effects of Saccharomyces albicans (S. albicans) on the cell cycle distribution and proliferation of human umbilical vein endothelial cell ECV304 cells in vitro.

METHODSThe line of ECV304 cultured in vitro were divided into four groups which were treated by S. albicans supernatant, S. albicans inactivated bacilli, supernatant and inactivated bacilli mixture, normal culture medium. The proliferous effect of ECV304 induced by supernatant, inactivated bacilli, supernatant and inactivated bacilli mixture using the methods of MTT, cell count, microscope and flow cytometry were conducted.

RESULTSIn the condition of different times and different culture concentrations, ECV304 cells incubated with 4-fold diluted S. albicans supernatant for 48 h increased the proliferation rate. The S and G2/M population of ECV304 cells increased after incubated with S. albicans supernatant for 40 h, which showed significant increasing cell proliferation index (PI) (P < 0.05). The PI of the cells treated by inactivated bacilli showed no significant change (P > 0.05).

CONCLUSIONS. albicans could induce ECV304 cell proliferation which depends on the release of metabolic products of S. albicans.

OBJECTIVETo evaluate the effects of non-Saccharomyces albicans metabolic products on the cell cycle distribution and proliferation of human umbilical vein endothelial cell ECV304 cells in vitro.

METHODSThe parallel dilution supernatant of Saccharomyces tropicalis, Saccharomyces krusei and Saccharomyces glabrata were prepared, and 1, 4, 16-fold(s) diluted concentration and control group were set up. The line of human umbilical vein endothelial cell ECV304 was cultured in vitro and treated by non-Saccharomyces albicans supernatant. The proliferous effect of ECV304 induced by non-Saccharomyces albicans supernatant after 24, 48, 72 h was detected by the methods of MTT, and the changes of cell density and cycle after 48 h were investigated by inverted microscope and flow cytometry.

RESULTSAt the 24th hour, all of the higher concentration (1-fold) of non-Saccharomyces albicans supernatant and the 4-folds diluted Saccharomyces krusei could promote ECV304 proliferation(P < 0.05). After adding various non-Saccharomyces albicans supernatant at 48h and 72th hour, Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant significantly increased proliferation rate of ECV304, while Saccharomyces tropicalis supernatant group showed no significant change no matter which concentration was tested. At 48th hour after adding the non-Saccharomyces albicans supernatant, the ECV304 cells density treated by Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant were significantly higher under the inverted microscope. The G0/G1 population of ECV304 cells decreased while cell proliferation index (PI) increased after incubated with Saccharomyces krusei supernatant and Saccharomyces glabrata supernatant for 48 hours (P < 0.05). Saccharomyces tropicalis group showed no significant change (P > 0.05).

CONCLUSIONThe metabolic products of Sacharoymces krusei and Saccharomyces glabrata could induce proliferation of ECV304 cell, which suggests non-Saccharomyces albicans should be undergone more attention clinically in detection and treatment.

Umbilical catheters have been used in NICUs for drawing blood samples, measuring blood pressure, and administering fluid and medications for more than 50 years. When the patient does not need the umbilical catheter or complications associated with umbilical catheters have risen, the catheter must be removed. In this process, the catheter may snap or be cut off and the fragment may migrate to a near vessel or to the heart and cause infection, thrombosis, or arrythmia. We report a case where in the process of removing an umbilical vein catheter, the catheter was stuck to the dried umbilical cord and pulling at it caused the catheter to snap. An immediate roentgenogram showed the fragmented catheter had migrated to the left pulmonary artery. Using an intravascular snare with a femoral approach, we were able to collect the remaining catheter and remove it from the patient's body without any complications.
Arrhythmias, Cardiac ; Blood Pressure ; Catheters* ; Heart ; Humans ; Pulmonary Artery ; SNARE Proteins* ; Thrombosis ; Umbilical Cord ; Umbilical Veins*