No abstract available.
Acute Lung Injury* ; Oxygen* ; Sus scrofa*

OBJECTIVETo evaluate the effect of wound closure tension on the blood flow of the expanded pedicled fasciocutaneous flap, so as to find the best tension for the blood supply of the flap.

METHODS8 piglets, aged 9-12 months, were used. On each side of pig back, a cephalic based expanded fasciocutaneous flap was designed. The size of the flaps was 5 cm x 20 cm. In the control group, the flaps were not elevated (0 g tension). In the experimental group, the flaps were treated at the end with the tension of 100, 200, 300, 400, 500, 600, 700, 800, and 900 g after elevation. The blood oxygen saturation (StO2) of the flaps was measured under different wound closure tension.

RESULTSThe flap StO2 raised along the increase of tension until the tension reached 200-300 g. Then the flap StO2 decreased when the tension continued to increase above 200-300 g.

CONCLUSIONSThe tension of 200-300 g is good for the blood flow of expanded pedicled fasciocutaneous flap. So the flap flexibility can be best used and the complication can be avoided.

Animals ; Surgical Flaps ; blood supply ; Sus scrofa ; Swine ; Tensile Strength

PURPOSE: This study was performed to evaluate any difference in the penetration of the acrylic glue into the pig rete mirabile according to the difference of the concentration of the glue mixture. MATERIALS AND METHODS: Three domestic pigs (12-15kg) were used. After obtaining a normal angiography, two different concentrations of 1:1 and 0.5:1 of Histoacryl Blue mixture with Lipiodol were administrated into pig rete mirabile and then anglographic and pathologic findings were analyzed after taking a postembolization angiography. RESULTS: The degree of the penetration of the glue mixture into the pig rete was different in the anglographic and pathologic findings according to the glue mixture ratio. In case of using 0.5:1 mixture of glue and Lipiodol, glue penetrated into the smaller vessels with size of about 80 micro rn than in case of using 1:1 mixture of glue and Lipiodol. CONCLUSION: Because the polymerization time of glue mixture will be different according to the concentration of glue mixture, a mixture with thin glue concentration seemed to penetrate deeply into the pig rete.
Adhesives ; Angiography ; Enbucrilate* ; Ethiodized Oil ; Polymerization ; Polymers ; Sus scrofa

Aujeszky's disease caused by Aujeszky's disease virus (ADV) is one of the most important diseases in the pig industry. In this study, we conducted a seroepidemiological survey of ADV in wild boars and raccoon dogs in South Korea. In total, 217 wild boar sera collected between March and August 2013, and 96 raccoon dogs between 2011 and 2012 were screened for the presence of antibodies against ADV. The sero-positive rates in wild boars and raccoon dogs tested for ADV were found to be 3.55% (8/225) and 0% (0/96), respectively. The presence of virus neutralization antibody titer against ADV means that small number of wild boars was infected with ADV and AD may be circulated continuously in Korean wild boar populations, and that wild boars may act as a potential reservoir of ADV. Therefore, to achieve the declaration of AD free, effective preventive measures to block transmission of AD should be taken to the wild boars.
Antibodies ; Herpesvirus 1, Suid* ; Korea ; Pseudorabies ; Raccoon Dogs* ; Sus scrofa*

No abstract available.
Acute Lung Injury* ; Prostaglandin-Endoperoxide Synthases* ; Sus scrofa*

Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15.0%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analyzed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), 2 as lineage 4 (PAstV-4), 2 identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to a bat astrovirus lineage. This first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.
Avastrovirus ; Chickens ; Reverse Transcriptase Polymerase Chain Reaction ; Sus scrofa ; Swine

BACKGROUNDThe influences of intrathoracic pressure (ITP) to hemodynamic and respiratory parameters during cardiopulmonary resuscitation (CPR) are confusing. In this research, we investigated the phasic changes of ITP during CPR and reveal the relationships among the hemodynamics, respiratory parameters, and ITP.

METHODSAfter 8 minutes of untreated ventricular fibrillation, which was induced in twenty intubated male domestic pigs, 12 minutes of 30: 2 CPR was performed. Continuous respiratory variables, hemodynamics, ITP and blood gas analysis were measured during CPR. After that, defibrillation was done and prognostic indicators after CPR was recorded.

RESULTSAverage ITP at baseline was -(14.1 ± 1.6) mmHg (1 mmHg = 0.133 kPa). When gasping inspirations were going on, it decreased sharply to near -50 mmHg. ITP fluctuated up and down quickly from near -20 mmHg to 20 mmHg when compressions were performed. These phasic changes became mild as the CPR was performed, the contrast of high and low ITP decreased to (12.95 ± 2.91) mmHg at the end of 12 minutes of CPR. Total alveolus minute volume decreased too, because of the decrease of compression and gasp related ventilations. Curve correlation was found between the tidal volume of compression and ITP: ITP = 607.33/(1 + 3134 × e(-0.58 × TV)), (e: natural constant, R(2) = 0.895). Negative correlations were found between the right atrial diastolic pressure and ITP (r = -0.753, P < 0.01); and positive correlations were found between the coronary perfusion pressure and ITP (r = 0.626, P < 0.01).

CONCLUSIONSITP is one of the key factors which can influence the prognosis of CPR. Correlations were found between the changes of ITP and the tidal volumes of compressions, right atrial diastolic pressure and coronary perfusion pressure during CPR. More positive ITP during compression and more negative during decompression were good to ventilation and perfusion.

Animals ; Blood Gas Analysis ; Cardiopulmonary Resuscitation ; Heart Arrest ; physiopathology ; therapy ; Hemodynamics ; Male ; Pressure ; Respiration ; Sus scrofa

Forkhead box (Fox) proteins play critical roles in the regulation of differentiation, proliferation, immunity and aging of cells. Most studies on Fox proteins are limited to cultured cells and rodent. The aim of the current study is to detect by immunohistrochemistry whether FoxO1, FoxO3a and FoxO4 proteins are localized in the stomach and intestine of the pig. The results showed that FoxO4 exists in the mucosa in all parts of the stomach and intestine; FoxO3a exists mainly in the lamina propria and muscularis of some parts. However, FoxO1 is not detectable in all parts of the stomach and intestine. Collectively, the results of the present study indicate that there exists a distinct expression pattern of Fox proteins, and that FoxO4 is a primary forkhead transcriptional factor localized in the gastrointestinal tracts of the pig.
Animals ; Female ; Forkhead Transcription Factors ; metabolism ; Gastrointestinal Tract ; metabolism ; Immunohistochemistry ; Male ; Sus scrofa ; metabolism ; Tissue Distribution

PURPOSE: The purpose of this study was to develop porcine full thickness skin wound healing model of thermal burn and skin defect and to compare wound healing process between them. METHODS: Twelve thermal burns and 12 skin defect wounds were created on the back of 3 domestic pigs. A round shaped heated electric iron was contacted for 30 seconds to develop full thickness burn. Appropriate dressings were applied for 30 days after wounding. Full thickness skin biopsies were obtained for histologic analysis by a pathologist. The amount of wound discharge, the surface area of each wound, and wound culture results were compared between skin defect and burn wound. RESULTS: Of 12 burn wound sections, 7 (58%) were identified to full thickness dermal injury. Complete wound reepithelialization was seen between postburn days 21 and 28 in both wounds. The initial wound area was smaller in burn than skin defect (P<0.001, 14.4+/-0.8 cm2 vs 22.8+/-3.4 cm2). The wound area of both burn and skin defect was slightly increased for 9 days after wounding and rapidly decreased after that time. The linear advancement length of wound edge was significantly slower in burn (P=0.009, 1.06+/-0.28 vs 1.48+/-0.42 cm). The amount of wound discharge was greater in skin defect than burn (P=0.002, 35.1+/-8.3 vs 49.5+/-11.2 g). CONCLUSION: We developed a porcine model of both thermal burn and skin defect. Time for complete reepithelialization was similar but the linear advancement length of wound edge was slower in burn than skin defect wound.
Bandages ; Biopsy ; Burns ; Hot Temperature ; Iron ; Models, Animal ; Re-Epithelialization ; Skin ; Sus scrofa ; Wound Healing

PURPOSE: Rabies viruses (RABV) circulating worldwide in various carnivores occasionally cause fatal encephalitis in swine. In this study, the safety and immunogenicity of a recombinant rabies virus, the ERAGS strain constructed with a reverse genetics system, was evaluated in domestic pigs. MATERIALS AND METHODS: Growing pigs were administered 1 mL (108.0 FAID50/mL) of the ERAGS strain via intramuscular (IM) or oral routes and were observed for 4 weeks' post-inoculation. Three sows were also inoculated with 1 mL of the ERAGS strain via the IM route. The safety and immunogenicity in swine were evaluated using daily observation and a virus-neutralizing assay (VNA). Fluorescent antibody tests (FAT) for the RABV antigen and reverse transcriptase-polymerase chain reaction (RT-PCR) assays for the detection of the nucleocapsid (N) gene of RABV were conducted with brain tissues from the sows after necropsy. RESULTS: The growing pigs and sows administered the ERAGS strain did not exhibit any clinical sign of rabies during the test period test and did develop VNA titers. The growing pigs inoculated with the ERAGS strain via the IM route showed higher VNA titers than did those receiving oral administration. FAT and RT-PCR assays were unable to detect RABV in several tissues, including brain samples from the sows. CONCLUSION: Our results suggest that the ERAGS strain was safe in growing pigs and sows and induced moderate VNA titers in pigs.
Administration, Oral ; Brain ; Encephalitis ; Nucleocapsid ; Rabies virus* ; Rabies* ; Reverse Genetics ; Sus scrofa ; Swine* ; Vaccines