This study was performed to evaluate the adsorption of beta2-microglobulin(beta2 M) by blood dialysis membrane materials which are polyethersulfone (PES), sulfonated polyethersulfones, (PES-SO3Na-I and PES-SO3Na-I ) in vitro incubated in human serum and radiolabeled beta2M (125I-beta2 M) solution respectively. In these experiments, the materials were incubated in 125I-beta2 M solution and human serum at the appointed time ranging from 15 minutes to four hours at 37 degrees C, and then the amounts of 125I-beta2M and serum beta2M adsorbed by materials were measured by radioimmunoassay (RIA). In the 125I-beta2 M system, amounts of 125I-beta2M adsorbed by the materials decreased in sequence of PES-SO3 Na-II > PES-SO3Na-I > PES. In the serum system, amounts of beta2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES-SO3Na-II > PES-SO3Na-I > PES. Sulfonated PES removes beta2M more than PES does and the adsorption of beta2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of beta2M may result in less beta2M available for incorporation into amyloid. The use of PES-SO3Na membranes lessens the likelihood of dialysis-related amyloidosis (DRA) development, which remains a major source of morbidity for patients treated with long-term hemodialysis.
Adsorption ; Amyloidosis ; blood ; Humans ; Polymers ; chemistry ; Renal Dialysis ; adverse effects ; Sulfones ; chemistry ; beta 2-Microglobulin ; blood

A new method of using photoactivable ester with azido group was described to immobilize urease on polyether sulfone(PES) film surface. The effects of photoactive enzyme concentration, temperature, pH, irradiation time on the activity of immobilized urease were investigated. Reused times and storage stability were also studied. The results showed that the surface concentration of urease immobilized on PES surface was about 0.33 mg/cm2. When the irradiation time was 5 minutes, the relative activity of immobilized urease was the highest and the activity increased with the increase of the concentration of photoactive urease solution. The optimum pH and temperature of immobilized urease were 7 and 50 degrees C respectively. The relative activity of immobilized urease was stable (50%) after 12 times reused at 50 degrees C.
Enzyme Stability ; Enzymes, Immobilized ; metabolism ; radiation effects ; Membranes, Artificial ; Photochemistry ; Polymers ; Sulfones ; Urease ; metabolism ; radiation effects

OBJECTIVE: To evaluate the acute toxicity of rofecoxib during concurrent use with cisplatin-based chemoradiotherapy (CCRT) in patients with cervical cancer. METHODS: We evaluated 67 FIGO stage IB2-IVA cervical cancer patients treated with CCRT between June 2002 and July 2004. The study group included patients who received rofecoxib (N=30) and the control group included patients who received CCRT only (N=37). The patients' medical records were retrospectively reviewed for patient characteristics, toxicity related to CCRT and treatment results. RESULTS: There were no significant differences in toxicity between the two groups. The most common acute grade 3/4 toxicity was neutropenia (13.3% in the study group and 21.6% in the control group). Grade 3/4 late toxicity was observed in 2 (6.6%) patients in the study group and 3 (8.1%) in the control group. There was no treatment-related deaths in either group. Six (20.0%) patients in the study group had treatment failure. In the control group, 6 (16.2%) patients experienced treatment failure. Progression-free and overall survival was 55.8+/-4.2 and 59.0+/-2.8 months, respectively, in the study group, and 69.7+/-4.3 and 71.6+/-3.6 months, respectively, in the control group. There were no differences in progression-free and overall survival between the 2 groups. CONCLUSION: Our data indicate that rofecoxib, at a dose of 25 mg twice daily, has acceptable acute toxicity as a radiosensitizer during CCRT. Although rofecoxib was not efficacious as a radiosensitizer in the present study, the benefit of rofecoxib as a radiosensitizer should be further evaluated in a prospective study.
Chemoradiotherapy ; Cyclooxygenase 2 ; Humans ; Lactones ; Medical Records ; Neutropenia ; Retrospective Studies ; Sulfones ; Treatment Failure ; Uterine Cervical Neoplasms

Apoptosis ; drug effects ; Carcinoma, Hepatocellular ; pathology ; Cell Line ; Hep G2 Cells ; Humans ; Lactones ; pharmacology ; Sulfones ; pharmacology

It has generally been assumed that erectile dysfunction (ED) is a shared sexual dysfunction that is distressing both for the men with ED and for their partners. Many ED men feel discouraged, disappointed or depressed. An oriental concept that the man has a leading role and full responsibility for sex may cause the impact of ED to be more serious for men than for women A woman's response to ED will vary depending on whether or not she desires to have intercourse with her male partner. If the woman desires intercourse, then she will fine herself frustrated, interpreting he no longer loves her and she will usually be quite hostile and become depressed. However, in case she is no longer interested in having intercourse with him, she may be secretly pleased at his sexual dysfunction and do nothing to alter the situation. Men with ED are reluctant to talk about their sexual problems to their partners mainly because of their pride and their reluctant to go to hospital due to the belief that ED is a natural process of aging or it is an occasional symptom with the hope that the problem will get better on its own, and it is not serious enough to go to the hospital. However, the number of patients seeking medical treatment of ED has increased since the launch of Viagra. ED treatment improves confidence, it brings a sense of emotional stability and vitality of life and marital life, and it improves the family and personal relations both for ED patients and their female partners.
Aging ; Erectile Dysfunction ; Female ; Humans ; Love ; Male ; Piperazines ; Purines ; Spouses ; Sulfones

PURPOSE: This study aimed to investigate the sexual attitudes and perception of sexual relationships and erectile dysfunction treatment among the wives of the long-term users of sildenafil (Viagra(R)). MATERIALS AND METHODS: The study was conducted as a market survey with using random face-to-face interviews and a structured questionnaire on a population of 31 wives (mean age; 49.8 years) of long-term sildenafil users who had first taken sildenafil 5 years or more previously and they continued its use in the previous 6 months. RESULTS: All the wives said 'It is desirable to have sex even though I am getting old, if I am healthy' and 84% had sex not for their satisfaction but for husband's satisfaction. Forty five percent perceived that having sex 1~2 times a week was ideal and 35% said having sex once per two weeks was ideal, and this was not different from happened in their real lives. Ninety four percent and 90% perceived that erectile dysfunction and women's sexual dysfunction should be treated, respectively, and 35% took a form of hormonal replacement therapy. The most influencing factors of sildenafil use on the overall satisfaction of wives' sexual and marital life were the increased frequency and intensity of orgasm and they became more intimate with their husbands, followed by the disappearance of depressed or annoying moods (p<0.01). They also felt the improved rigidity of their husband's erection after initial sildenafil use was maintained after more than 5-year use. CONCLUSIONS: Wives of long-term sildenafil users had positive, but husband-oriented sexual attitudes. They had an intercourse-focused perception on their sexual relationship, and they ranked the increased frequency and intensity of their orgasms and becoming more intimate with husbands as the most influential factors for their sexual and marital life, and they also felt that their husbands' erection rigidity was maintained after more than 5-year use of sildenafil.
Erectile Dysfunction ; Female ; Humans ; Male ; Orgasm ; Piperazines ; Purines ; Sildenafil Citrate ; Spouses ; Sulfones

BACKGROUND: Vardenafil is a phosphodiesterase type 5 inhibitor, used in erectile dysfunction. This study aimed to evaluate the pharmacokinetics and tolerability of vardenafil following a single oral administration in healthy male subjects. METHODS: A randomized, double-blind, placebo-controlled, single dosing, dose-escalation study was conducted in 30 healthy subjects. A single oral dose of vardenafil or placebo was given to 10 subjects (8 active + 2 placebo) in each dose group of 5, 10 and 20 mg. Serial blood and urine samples were obtained up to 48 hours for pharmacokinetic analysis. Vardenafil and its metabolite were detected by high performance liquid chromatography tandem mass spectrometry assay. RESULTS: A total of 45 adverse events (AE) were reported in 22 subjects, including 5 AEs from placebo treatment, and all the AEs were mild, except one case of moderate nasal stuffiness. Vardenafil was absorbed after a single oral dose, with the tmax of 0.5-1.0 hours. The Cmax and AUClast were 10.21 +/- 3.68 ug/L(mean +/- SD) and 18.08 +/- 7.44 ugxh/L in 5 mg dose group, 19.79 +/- 12.13 ug/L and 38.61 +/- 21.04 ugxh/L in 10 mg dose group and 53.16 +/- 37.01 ug/L and 110.05 +/- 69.65 ugxh/L in 20 mg dose group. Dose-linearity on AUClast and Cmax of vardenafil were observed in three dose groups. In all dose groups, the fraction excreted in urine was less than 1%. CONCLUSION: The vardenafil was tolerable over a single dose range of 5 - 20 mg. The pharmacokinetics of vardenfil after a single oral dose was explored and linear pharmacokinetic characteristics were observed over the dose range of 5 - 20 mg in healthy subjects.
Administration, Oral ; Chromatography, Liquid ; Erectile Dysfunction ; Humans ; Imidazoles ; Male ; Piperazines ; Sulfones ; Tandem Mass Spectrometry ; Triazines

Bullous pemphigoid is a chronic and relatively benign subepidermal blistering disease and is generally considered not to be a sulfa-responsive dermatosis. As a rule, bullous dermatosis that excellently responded to sulfapyridine and sulfones was considered by some to be diagnostic of dermstitis herpetiformia. In 1977, Person and Rogers described 6 cases of bullous pemphigoid responded to sulfapyridine and sulfones. These sulfa-responsive cases were younger than ordinary bullous pemphigoid patients and the histopathologic findings showed prevalence of the neutrophilic infiltration. In this report, the patient who diagnosed as bullous pemphigoid by immunofluorescent studies has younger onset (38 years of age) than usual bullous pemphigoid and histopatbologic findings showed dense neutrophilic infiltrations. The patient was treated with 100-200mg of DDS for 2 months and the skin lesions completely disappeared.
Blister ; Humans ; Neutrophils ; Pemphigoid, Bullous* ; Prevalence ; Skin ; Skin Diseases ; Sulfapyridine ; Sulfones

Pityriasis lichenoides et varioliformis acuta is a cutaneous disease of unknown origin characterized by an acute polymorphous eruption which heals with superficial scarring and pigmentation. It occurs mainly in young adults and less commonly in children. Although various types of therapy have been proposed for pityriasis lichenoides, including systemic steroids, tetracycline, methotrexate, sulfones, an UV radiation, there is no definitive treatment and there is few studies of appropriate, efficacious therapy in children. We report a case of pityriasis lichenoides et varioliformis acuta in children treated with roxithromycin.
Child* ; Cicatrix ; Humans ; Methotrexate ; Pigmentation ; Pityriasis Lichenoides* ; Pityriasis* ; Roxithromycin* ; Steroids ; Sulfones ; Tetracycline ; Young Adult

Pityriasis lichenoides et varioliformis acuta is a cutaneous disease of unknown origin characterized by an acute polymorphous eruption which heals with superficial scarring and pigmentation. It occurs mainly in young adults and less commonly in children. Although various types of therapy have been proposed for pityriasis lichenoides, including systemic steroids, tetracycline, methotrexate, sulfones, an UV radiation, there is no definitive treatment and there is few studies of appropriate, efficacious therapy in children. We report a case of pityriasis lichenoides et varioliformis acuta in children treated with roxithromycin.
Child* ; Cicatrix ; Humans ; Methotrexate ; Pigmentation ; Pityriasis Lichenoides* ; Pityriasis* ; Roxithromycin* ; Steroids ; Sulfones ; Tetracycline ; Young Adult