1.Detection of human papillomaviruses in cervical interepithelial neoplasia and invasive carcinoma by in situ polymerase chain reaction.
Joon Cheol PARK ; Tae Sang KIM ; Dong Ja KIM ; Han Ik BAE ; Jeong Ran KIM
Korean Journal of Obstetrics and Gynecology 2000;43(10):1738-1743
No abstract available.
Humans*
;
Polymerase Chain Reaction*
2.Detection of HPV in cervical scrape specimens of cervical neoplasia using the polymerase chain reaction.
Seung Chul KIM ; Hak soon KIM ; Ju Cheol SONG ; Seo Ok KANG ; Young Bum CHA ; In Kwon HAN ; In Geol MOON ; Won Hee HAN ; Chong Taek PARK
Korean Journal of Obstetrics and Gynecology 1992;35(9):1269-1279
No abstract available.
Polymerase Chain Reaction*
3.Detection of hepatitis viral nucleic acid sequences using polymerase chain reaction.
Korean Journal of Infectious Diseases 1991;23(4):229-233
No abstract available.
Hepatitis*
;
Polymerase Chain Reaction*
4.The Clinical Applicability of PCR and FISH in the Detection of Y-chromosome from Fetal Nucleated Red Blood Cells in Maternal Blood.
Jae Hyun CHUNG ; Kwan Ja JI ; Soon Ha YANG ; Jung Mi OH ; Cheong Rae ROH ; Young Kyu MOON ; Syng Wook KIM ; Je Ho LEE
Korean Journal of Obstetrics and Gynecology 1997;40(12):2692-2697
No abstract available.
Erythrocytes*
;
Polymerase Chain Reaction*
5.Detection of male-specific DNA by polymerase chain reaction.
Korean Journal of Perinatology 1993;4(3):391-400
No abstract available.
DNA*
;
Polymerase Chain Reaction*
6.Determination of sex by polymerase chain reaction (I).
Sang Hun CHA ; Tai Ho CHO ; Yong Sang SONG ; Hyo Pyo LEE
Korean Journal of Obstetrics and Gynecology 1991;34(11):1568-1573
No abstract available.
Polymerase Chain Reaction*
7.Rapid determination of fetal Y-chromosome with polymerase chain reaction.
Sung Ho KANG ; Kyu Byung JUNG ; Ho Won HAN ; Young Chul KIM ; Sung Il NOH ; Ki Suk OH ; In Kwon HAN ; In Gul MOON
Korean Journal of Obstetrics and Gynecology 1993;36(3):321-325
No abstract available.
Polymerase Chain Reaction*
8.Some application of PCR in microbiology
Journal of Practical Medicine 2002;435(11):25-27
In microbiology, PCR was applied very early and widely step by step to diagnose the etiology of the infection. Especially in case of the culture of microorganism was unsuccessfully implemented or is very difficult or patient used the antibiotic before admission because PCR can be implemented in the dead microorganism. PCR contributes to verify more correctly.
microbiology
;
Polymerase Chain Reaction
9.Sequencing of Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium and application for PCR to differentiate them
Journal of Preventive Medicine 2005;15(5):36-41
All Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium code for phase 1 of H antigen (H:1) (d, a, b, c and i antigen respectively). The genes were sequenced on Sanger's principle by automatic sequenser (ABI, 3100 Avant, Genetic Analyser). The primer pairs for the Salmonella species were designed on the basis of the collected sequences. The results showed that PCR with these primers can clearly differentiate the five Salmonella strains, especially between S. paratyphi B and S. typhimurium.
Polymerase Chain Reaction
;
Salmonella
10.Optimisation of polymerase chain reaction conditions for detection of mineralization markers in isolated odontoblasts from human teeth
Wafa’ Zaharia ; Ong Wei Shena ; Tan Hong Jina ; Saaid Al Shehadatb ; Azlina Ahmada ; Khairul Bariah Ahmad Amin Noordina
Archives of Orofacial Sciences 2017;12(1):45-54
The present study aimed to determine the best polymerase chain reaction (PCR) conditions for
amplification of odontoblast markers; alkaline phosphatase (ALP), dentin matrix protein 1 (DMP1), dentin
sialophosphoprotein (DSPP) and osteopontin (OPN). Informed consent was obtained from the individuals
prior to tooth extraction. RNA was extracted from odontoblasts obtained from extracted teeth using
innuPREP RNA Mini kit (Analytik Jena, Germany). Five selected target factors in enhancing PCR: primer
concentration, extension time, number of cycles, annealing time, and annealing temperature were
manipulated to yield the correct size of amplicons. One step reverse transcriptase PCR reactions were
performed using MyTaq One-Step RT-PCR kit (Bioline, USA) with a C1000 Thermal Cycler (Bio-Rad, USA)
in a 25 µL reaction, keeping the amount of 2 ng/µL RNA, 0.25 µL reverse transcriptase, 0.5 µL RiboSafe
Rnase inhibitor and 1X MyTaq One-Step Mix, constant. The optimal conditions were determined to be
400nM of primers for DMP1 and DSPP, 200 nM for ALP and OPN; 30 seconds of extension time and 35
PCR cycles for all genes; 10 seconds of annealing time for ALP, DMP1 and DSPP, 7 seconds for OPN. The
annealing temperature were 56.4°C for ALP, 58.6°C for DMP1, 52.7°C for DSPP, and 56.3°C for OPN,
respectively. The optimized PCR protocols produced the correct size of odontoblast markers.
Polymerase Chain Reaction