OBJECTIVETo study the pollen morphology of ten species of Achillea.

METHODThe pollen morphology of ten species of Achillea was examined with LM and SEM.

RESULTThe pollen grains were usually 3-colporate, subspheroidal. The exine ornamentation consisted of verrucate, spinulate and foveolate. But some differences in size, colpae and exine ornamentation were found.

CONCLUSIONThe slight differences of the pollen morphology are useful to some extent for the classification of the ten species of Achillea.

Achillea ; classification ; ultrastructure ; Microscopy, Electron, Scanning ; Pollen ; ultrastructure ; Species Specificity

The daily pollen counts in the air of Seoul, Korea were measured using a rotorod sampler from June 1990 to July 1992. Two distinct pollen periods were noted: tree pollens such as alder, poplar, willow, oak and pine appeared in the spring from March to May, and weed pollens, such as Artemisia spp., Ambrosia spp. and Hop japanese appeared in the autumn from August to September. Various kinds of grass pollen appeared from June to September. The study indicated that there were two pollen peaks in Seoul composed of tree pollens in spring and weed pollens in autumn.
Air Pollution/*statistics & numerical data ; Allergens/*analysis ; Humans ; Korea ; Plants ; *Pollen/classification ; Seasons

OBJECTIVETo evaluate the species, quantity and diffusion of allergenic pollens as well as the relationship among allergic rhinitis (AR), pollen species and meteorological conditions in Kunming area.

METHODSThe exposure film was used to investigate the species of airborne pollen, the total number of these pollens during December 2009 to November 2010 by means of gravitational sedimentation, at the same time, AR patients were registered. The species of allergic pollen in Kunming area during the same period, and the statistic analysis was made with the meteorological elements, using the correlation analysis methods of Excel for data analysis.

RESULTSFrom December 2009 to November 2010, a total of 60 exposure films of pollen species was found, including 26 147 pollens. The annual airborne pollen showed two scattering peak, respectively: February to April and September to October; the number of advantage pollen was 17 species. The number of year-round drifting pollen was 13 species while 2 species showed two peaks on spring and autumn. There was another 26 new species added to the results of 2009-2010, compared with 1987-1989, while the original 12 species were not seen. The drifting peak was the same. The number of allergen intradermal tests (AIT) positive AR patients was positively correlated with 16 pollen species, respectively, oleaceae, alnus nepalensis, walnut, palm, pig weed, fennel, Amaranthaceae, artemisia, rosa, Albizia julibrissin Durazz, gramineae, castor, Fraxinus L, populus, Morus alba L, hemp humulus, (r value were 0.689 7, 0.550 1, 0.742 1, 0.755 2, 0.771 2, 0.663 3, 0.556 1, 0.568 3, 0.659 8, 0.607 4, 0.834 7, 0.664 3, 0.745 9, 0.634 7, 0.718 3, 0.777 2, all P < 0.05). The population of AIT positive AR patients was positively correlated with average temperature, average wind speed and other meteorological factors (r values were 0.552 0, 0.883 5, both P < 0.05), and was negatively correlated with average relative humidity (r = -0.714 7, P < 0.05).

CONCLUSIONSKunming had a large number of airborne pollen species which showed two peaks annually, respectively, spring and autumn. The relationship among the number of AIT positive AR patients, the meteorological factors and the number of airborne pollen were close.

Allergens ; analysis ; Humans ; Meteorological Concepts ; Pollen ; classification ; Rhinitis, Allergic, Seasonal ; diagnosis ; Seasons ; Temperature

DNA barcoding method was conducted for the authentication of pollen materials due to difficulty of discriminating pollen materials bearing morphological similarity. In this study, a specific focus was to identify cattail pollen (Puhuang) and pine pollen (Songhuafen) samples from their adulterants which are frequently mixed-together. Regions of the internal transcribed spacer (ITS2) from 60 samples were sequenced, and new primers for cattail pollen were designed according to the sequence information. The results from the NJ trees showed that the species of pine pollen, Puhuang and their adulterants can be classified as obvious monophyly. Therefore, we propose to adapt DNA barcoding methodology to accurately distinguish cattail pollen, pine pollen and their adulterant materials. It is a great help for drug regulatory agency to supervise the quality of medicinal materials.
China ; DNA Barcoding, Taxonomic ; methods ; DNA, Plant ; genetics ; DNA, Ribosomal Spacer ; genetics ; Drug Contamination ; prevention & control ; Drugs, Chinese Herbal ; chemistry ; classification ; Molecular Sequence Data ; Phylogeny ; Pinus ; classification ; genetics ; Pollen ; classification ; genetics ; Quality Control ; Typhaceae ; classification ; genetics

Allergic rhinitis is chronic disorder of nose which is evoked by IgE-mediated inflammation mostly triggered by contact between nasal mucosa and inhalant allergen. Allergic rhinitis can be classified according to seasonality (seasonal or perennial), continuity (intermittent or persistent), and severity (mild, moderate, or severe). Allergic rhinitis is relatively common, and the prevalence of allergic rhinitis has increased in Korea like other countries. As well as the prevalence of allergic rhinitis, the indoor and outdoor concentration of inhalant allergens and the sensitization to inhalant allergens were systematically investigated in Korea. As a result, house dust mite, pollens, fungal spores, cockroach, and animal hairs were found to be common inhalant allergens. Moreover, the concentration of tree pollens and grass pollens and sensitization to those pollens increased because of global warming, which may consequently increase the prevalence of pollen-induced allergic rhinitis. With regard to socioeconomic burden of allergic rhinitis, the direct medical cost including herbal and alternative medicine was as high as 1.78 million Korean won per year. Direct nonmedical cost as well as indirect cost including unproductivity, absenteeism, and medical cost for associated diseases such as asthma worsened the socioeconomic burden of allergic rhinitis.
Absenteeism ; Allergens ; Animals ; Asthma ; Classification* ; Cockroaches ; Complementary Therapies ; Epidemiology* ; Global Warming ; Hair ; Inflammation ; Korea ; Nasal Mucosa ; Nose ; Poaceae ; Pollen ; Prevalence ; Pyroglyphidae ; Rhinitis* ; Rhinitis, Allergic, Perennial ; Seasons ; Spores, Fungal

Allergic rhinitis is chronic disorder of nose which is evoked by IgE-mediated inflammation mostly triggered by contact between nasal mucosa and inhalant allergen. Allergic rhinitis can be classified according to seasonality (seasonal or perennial), continuity (intermittent or persistent), and severity (mild, moderate, or severe). Allergic rhinitis is relatively common, and the prevalence of allergic rhinitis has increased in Korea like other countries. As well as the prevalence of allergic rhinitis, the indoor and outdoor concentration of inhalant allergens and the sensitization to inhalant allergens were systematically investigated in Korea. As a result, house dust mite, pollens, fungal spores, cockroach, and animal hairs were found to be common inhalant allergens. Moreover, the concentration of tree pollens and grass pollens and sensitization to those pollens increased because of global warming, which may consequently increase the prevalence of pollen-induced allergic rhinitis. With regard to socioeconomic burden of allergic rhinitis, the direct medical cost including herbal and alternative medicine was as high as 1.78 million Korean won per year. Direct nonmedical cost as well as indirect cost including unproductivity, absenteeism, and medical cost for associated diseases such as asthma worsened the socioeconomic burden of allergic rhinitis.
Absenteeism ; Allergens ; Animals ; Asthma ; Classification* ; Cockroaches ; Complementary Therapies ; Epidemiology* ; Global Warming ; Hair ; Inflammation ; Korea ; Nasal Mucosa ; Nose ; Poaceae ; Pollen ; Prevalence ; Pyroglyphidae ; Rhinitis* ; Rhinitis, Allergic, Perennial ; Seasons ; Spores, Fungal

One hundred and one cases with allergic rhinitis were statistically analysed and 40 students without any allergic symtoms as control group were chosen and nasal eosinophile count were performed. The following results were obtained: 1. Among 101 allergic cases the male and female ratio was 4:5. 2. In 54.5%(55 cases) of the allergic group and 15%(6 cases) of the control group showed a positive reaction in the allergic skin test, but no significant sex difference was found. 3. In the 55 allergy positive cases. the peak incidence was in the 4th decade (27.3%) and next in order was the 3rd and 5th decade age group (21.8%). 4. Twenty six causative allergens by skin test were identified and the most frequent allergens were the animal hairs (43.3%) pre dominantly cat, dog and horse and next in frequency were the pollens (34.7%) and house dust (14.0%). 5. The most sensitive allergic reaction was to the house dust and appeared before the 5th year, animal hairs from 6~9 years, and pollens in the 2nd~3rd decade groups. 6. Among 150 positive reactions in 55 patients, ++(60) was the most frequent reaction level and next in order was +(58), +++(26) and ++++(6). 7. The allergic rhinitis due to food was only 2% which included two cases of mussel and a case of mushroom sensitivity. 8. Only 27% of the cases with positive skin, test showed single allergen sensitivity and 73% were multiple allergen sensitivity. 9. The most common combination of multiple allergens was between a variety of animal hairs, animal hairs and house dust, animal hairs and pollens, a variety of pollens and house dust in that order. 10. Among 101 cases of allergic rhinitis, 56.4% (57 cases) showed nasal eosinophilia and 64.9% (30 cases) of these revealed a positive skin test.
Adolescent ; Adult ; Aged ; Allergens/classification* ; Child ; Child, Preschool ; Dust ; Female ; Hair ; Hay Fever/immunology* ; Human ; Male ; Middle Age ; Pollen ; Skin Tests

Food allergy is an adverse food reaction as a result of immune mechanisms. In a sensitized individual, food allergens activate mast cells and basophils by binding with IgE present on the cell surface, resulting in the release of chemical mediators and various cytokines to cause various clinical symptoms of food allergy. Sensitization to food allergens can occur in the gastrointestinal tract (class 1 food allergy) or as a consequence of cross reactivity to structurally homologous inhalant allergens (class 2 food allergy). The class 1 food allergens are water-soluble glycoproteins with 10-70 kD size that are resistant to heat, acid and enzymes. On the other hand, the class 2 food allergens are highly unstable and degraded by heat or enzymatic digestion. Much progress has been made in identifying and isolating food allergen. Recently cDNAs for many proteins have been isolated and recombinant proteins have been generated. These techniques make it easier to characterize each responsible food allergens. Plant food allergens are classified into families and superfamilies by their structural and functional properties. The most of plant food allergens are the cupin and prolamin superfamilies and the protein families of the plant defense system. The cupin superfamily includes allergenic seed storage proteins of 7s globulin (vicilin) and 11s globulin (legumin). 2s albumin seed storage proteins, the nonspecific lipid transfer proteins, and the cereal alpha-amylase and protease inhibitors belong to the prolamin superfamily. Profilins, heveins, and nonspecific lipid transfer proteins are present in a variety of pollens, nuts, seeds, fruits, and vegetables. These are considered as panallergens, causing a significant degree of IgE-mediated cross-reactivity. Detailed informations about the character of food allergens can be used to develop more sophisticated diagnostic methods and treatment modalities in the near future. Further knowledge of food allergens is also useful to assess the allergenicity of novel protein of genetically mo.
Allergens* ; alpha-Amylases ; Basophils ; Edible Grain ; Classification* ; Cytokines ; Digestion ; DNA, Complementary ; Food Hypersensitivity ; Fruit ; Gastrointestinal Tract ; Glycoproteins ; Hand ; Hot Temperature ; Humans ; Immunoglobulin E ; Mast Cells ; Nuts ; Plants* ; Pollen ; Profilins ; Protease Inhibitors ; Recombinant Proteins ; Seed Storage Proteins ; Vegetables

OBJECTIVE: To investigate the allergens distribution of 576 allergic rhinitis patients in Qingyang, and to provide basic epidemiologic information for the prevention and treatment of allergic rhinitis. METHOD: Skin prick test was done to all the 576 patients with allergic rhinitis with 28 kinds of allergens. RESULT: Four hundred and eighty cases (83.3%) showed positive reaction to at least one allergen of 28 allergens. The most common allergens were Magwort (73.3%), Giant Ragweed (55.0%) Tree II (51.7%), Tree I (48.3%) Dermatophagoides farinae (43.3%) and Dermatophagoides pteronyssinus (36.7%). Moreover. the positivity decreased with age. There was no difference between male and female. CONCLUSION The study shows that Magwort. Giant ragweed and tree II are the most important allergens on Qingyang district.
Adolescent ; Adult ; Aged ; Allergens ; analysis ; classification ; Animals ; Child ; Child, Preschool ; China ; Female ; Humans ; Male ; Middle Aged ; Mites ; immunology ; Pollen ; immunology ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; diagnosis ; immunology ; Skin Tests ; Young Adult

INTRODUCTIONAllergic rhinitis (AR) is a prevalent disease worldwide but is still underdiagnosed in many parts of Asia. We studied the clinical profiles of AR patients in our community based on the new ARIA classification and investigated the aetiological allergens using a skin prick test.

MATERIALS AND METHODSIn 2008, 142 newly diagnosed patients with AR were seen and underwent skin prick testing with 90 patients completing the study.

RESULTSIntermittent mild and moderate/severe AR were evident in 10% and 21.1% of the patients, while persistent mild and moderate/severe were seen in 20% and 48.9%, respectively. Rhinitis and asthma co-morbidity occurred in 28.8% with asthma incidence significantly higher in persistent AR (P = 0.002). There was no significant association between AR severity, city living and asthma co-morbidity. Nasal itchiness and sneezing were the main presenting complaints and were more common in intermittent AR (P <0.05). Sleep disturbance was associated with moderate-severe AR (P <0.05). Polypoidal mucosa was associated with asthma co-morbidity (P <0.05). Monosensitivity reaction occurred in 12.2% of patients and was associated with fungi sensitivity (P <0.05). Majority of patients were oligosensitive (52.8%) and polysensitive (34.4%) and were significantly associated with moderate-severe persistent AR (P <0.01). The highest positive skin prick reaction and the largest average wheal diameter were for the house dust mites and cat allergen (P <0.05).

CONCLUSIONOur results reflected the AR profiles in our country, which was comparable with typical profiles of the neighbouring country and other Mediterranean countries with a similar temperate climate.

Adolescent ; Adult ; Aged ; Allergens ; Animals ; Asthma ; Cats ; Female ; Fungi ; Health Status Indicators ; Humans ; Incidence ; Malaysia ; epidemiology ; Male ; Middle Aged ; Pilot Projects ; Pollen ; Prevalence ; Pyroglyphidae ; Rhinitis, Allergic, Perennial ; classification ; diagnosis ; epidemiology ; pathology ; Severity of Illness Index ; Skin Tests ; Sleep Wake Disorders ; Statistics as Topic ; Time Factors ; Young Adult