2.Research progress on vascularization of organoids.
Junyi SHEN ; Zhi OUYANG ; Jian ZHONG ; Yicen LONG ; Yujia SUN ; Ye ZENG
Journal of Biomedical Engineering 2023;40(4):625-631
Organoids are three-dimensional structures formed by self-organizing growth of cells in vitro, which own many structures and functions similar with those of corresponding in vivo organs. Although the organoid culture technologies are rapidly developed and the original cells are abundant, the organoid cultured by current technologies are rather different with the real organs, which limits their application. The major challenges of organoid cultures are the immature tissue structure and restricted growth, both of which are caused by poor functional vasculature. Therefore, how to develop the vascularization of organoids has become an urgent problem. We presently reviewed the progresses on the original cells of organoids and the current methods to develop organoids vascularization, which provide clues to solve the above-mentioned problems.
Humans
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Organoids
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Neovascularization, Pathologic
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Technology
4.Relationship between thrombospondin-1 and the occurrence and development of oral and maxillofacial malignancy.
Xue-Qin ZHOU ; Jun REN ; Sen YANG
West China Journal of Stomatology 2018;36(6):686-690
Thrombospondin-1 (TSP-1) is widely distributed in human tissues and is important in inhibiting angiogenesis.It also occupies an indispensable position in the formation, growth, differentiation, and metastasis of tumors in different tissues.TSP-1 plays an important role in the occurrence and development of various types of tumors. The inhibitory effect of TSP-1 on the angiogenesis and tumor development of oral and maxillofacial malignant tumors has been demonstrated in recent years. This paper reviews the findings and progress of TSP-1 research involving all kinds of tumors as well as oral and maxillofacial malignancies.
Humans
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Neoplasms
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Neovascularization, Pathologic
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Thrombospondin 1
6.A three-dimension in vitro model for angiogenesis of hemangioma.
Cheng-Hong JIANG ; Fu-Lian ZHUANG ; Ba-Rui HUANG ; Zhi-Hui GUO ; Yi-De XIE ; Ya-Kuan ZHOU ; Biao WANG ; Wen-lie CHEN
Chinese Journal of Plastic Surgery 2005;21(5):364-367
OBJECTIVETo create a three dimension (3D) in vitro model for angiogenesis of hemangioma.
METHODThe fragment of hemangioma specimen was embedded in fibrin gel to set up the three-dimension (3D) in vitro model for angiogenesis of hemangioma.
RESULTIn the model, microvessels grew out from the tissue fragments at the 2nd to 3rd day after culture, and at the 8th to 9th day a compact network of microvessels come into being, then tending to be stationary. The compact network around the tissue fragment was confirmed to be blood vessels by immunohistochemistry and electron microscopy.
CONCLUSIONThis model helps to study the mechanism of hemangioma angiogenesis and investigate the drugs of anti-angiogenesis.
Cells, Cultured ; Endothelium, Vascular ; Hemangioma ; Humans ; Models, Cardiovascular ; Neovascularization, Pathologic
8.Biological Characteristics of Cluster of Differentiation 147 and Its Relationship with Tumour.
Cen GAO ; Chao-Hui LU ; Jie CHEN
Acta Academiae Medicinae Sinicae 2016;38(5):589-593
Cluster of differentiation 147(CD147)/extracellular matrix metalloproteinase inducer (EMMPRIN) is a widely distributed transmembrane glycoprotein that belongs to the immunoglobulin superfamily and is highly enriched on the surface of malignant tumour cells. A major function of CD147 is to stimulate matrix metalloproteinase production in stromal fibroblasts and endothelial cells. CD147 promotes growth,invasion,metastasis,and glycolysis of malignant cells,induces angiogenesis,multidrug resistance,and anoikis resistance,and inhibits starvation-induced autophagy et al. This review focuses on the structural and biological characteristics of CD147 as well as recent advances in its multiple functions in malignant tumours and underlining mechanisms.
Basigin
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metabolism
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Fibroblasts
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Humans
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Neoplasms
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metabolism
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Neovascularization, Pathologic
9.Effects of extracellular vesicles from various cell types on angiogenesis.
Lu REN ; Xiaofei ZHANG ; Siyuan TANG
Journal of Central South University(Medical Sciences) 2019;44(12):1423-1429
Extracellular vesicles (EVs), including apoptotic bodies, microvesicles and exosomes, play a crucial role in cell-to-cell communication. EVs derived from various cell types have the potential to deliver complex information to endothelial cells and to induce either pro- or anti-angiogenic signaling.
Cell-Derived Microparticles
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Endothelial Cells
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Extracellular Vesicles
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Humans
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Neovascularization, Pathologic
10.Advances in the research of mechanisms of promotion of vascularization by angiogenin and its application.
Xingang WANG ; Pan WU ; Chunmao HAN ; Email: HANCHUNMAO1@126.COM.
Chinese Journal of Burns 2015;31(6):473-475
Angiogenin, as a member of the ribonuclease superfamily, is an angiogenic protein. The angiogenic ability of angiogenin plays an important role in many physical and pathological processes. Angiogenin can induce endothelial cell migration, proliferation, tubulation, as well as inhibition of cellular apoptosis. Angiogenin can be used to modulate the angiogenetic process of tissue engineered constructions via local delivery. Furthermore, angiogenin can also be regarded as a biomarker for diagnostic evaluation of malignancy, or as a target for cancer therapy. This paper presents a comprehensive overview of the angiogenic mechanisms of angiogenin, as well as its potential application in the process of wound healing and treatment of ischemic diseases and malignancy.
Cell Movement
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Humans
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Neovascularization, Pathologic
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Neovascularization, Physiologic
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Ribonuclease, Pancreatic
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therapeutic use