No abstract available.
Basophils* ; Histamine Release* ; Histamine*

No abstract available.
Animals ; Histamine Release* ; Histamine* ; Mast Cells* ; Rats*

No abstract available.
Anaphylaxis* ; Histamine Release* ; Histamine* ; Mast Cells*

BACKGROUND: The fruit of Rubus coreanus (RC), a perennial herb, has been cultivated for a long time as a popular vegetable. The anti-allergy mechanism of RC is unknown. The purpose of this study is to investigate the inhibitory effect of RC on compound 48/80- or anti-DNP IgE-induced mast cell activation. METHODS: For this, influences of RC on the compound 48/80-induced degranulation, histamine release, calcium influx and the change of the intracellular cAMP (cyclic adenosine-3',5' monophosphate) levels of rat peritoneal mast cells (RPMC) and on the anti-DNP IgE-induced histamine release of RPMC were observed. RESULTS: The pretreatment of RC inhibited compound 48/ 80-induced degranulation, histamine release and intracelluar calcium uptake of RPMC. The anti-DNP IgE-induced histamine release of RPMC was significantly inhibited by pretreatment of RC. The RC increased the level of intracellular cAMP of RPMC, and the pretreatment of RC inhibited compound 48/80-induced decrement of intracellular cAMP of RPMC. CONCLUSION: These results suggest that RC contains some substances with an activity to inhibit the compound 48/80- or anti-DNP IgE-induced mast cell activitation. The inhibitory effects of RC are likely due to the stabilization of mast cells by blocking the calcium uptake and enhancing the level of intracellular cAMP.
Animals ; Calcium ; Fruit ; Histamine ; Histamine Release ; Mast Cells* ; Rats ; Vegetables

No abstract available.
Animals ; Guinea Pigs* ; Guinea* ; Heart* ; Histamine Release* ; Histamine*

No abstract available.
Animals ; Calcium* ; Histamine Release* ; Histamine* ; Mast Cells* ; Rats*

No abstract available.
Aeromonas hydrophila* ; Aeromonas* ; Animals ; Histamine Release* ; Histamine* ; Mast Cells* ; Rats*

We previously described a rare case of anaphylaxis presumably induced by carminic acid in cochineal dye used as a food additive. In this study, highly pure carminic acid was added to an albumin-containing buffer at various concentrations, followed by serial dilution. Varying the mixing ratio of carminic acid and albumin affected the extent of histamine release from passively sensitized basophils. Similar basophil histamine release occurred with carminic acid-globulin solutions. These results provide experimental evidence indicating that basophil activation is dependent on hapten (carminic acid) and carrier (protein) interaction.
Anaphylaxis ; Basophils ; Carmine ; Food Additives ; Histamine Release ; Hypersensitivity ; In Vitro Techniques

A case is reported of anaphylactoid reaction to atracurium, used for the induetion of an- esthesia for skin graft in a 36 year old man. The patient had no previous history of any allergic tendency. It is not possible to distinguish between direct pharmacological effects and immune mediated hypersensitivity reactions by clinical observation alone. The mechanism of reaction in this patient was investigated by the leukocyte histamine release test, intradermal test, direct intravenous challenge with other induction agents used during the induction of anesthesia and radioallergosorbent test. The histamine releasing property of atracurium, the distinguishing methods between ana phylactic and anaphylatoid reaction, and the anesthetic management for this kind of reaction are discussed.
Adult ; Anaphylaxis ; Anesthesia ; Atracurium* ; Histamine ; Histamine Release ; Humans ; Hypersensitivity ; Intradermal Tests ; Leukocytes ; Radioallergosorbent Test ; Skin ; Transplants

PURPOSE: The release of histamine from human basophils is controlled by an intrinsic, as yet unidentified, cellular property termed "releasability." We carried out this study to ascertain whether there was any difference in the releasability of basophils from asthmatic children compared to those from normal children. We intended also to clarify the correlation between the releasability and the atopic status of asthma. METHODS: We selected nineteen atopic asthmatic, eighteen nonatopic asthmatic and fourteen normal children for this study. Suspensions of leukocytes were isolated and stimulated with calcium ionophore A23187, anti-IgE and D. pteronyssinus antigen. After incubation, the supernatant was assayed for histamine with an automated fluorometric technique. RESULTS: Basophil histamine release with anti-IgE was different in three groups. Anti-IgE caused significantly more basophil histamine release in asthmatic children than in nomal children. Atopic asthmatic group showed greater basophil histamine release with anti-IgE than nonatopic asthmatic group. D. pteronyssinus antigen caused the significant amount of histamine release only in atopic asthmatic group. CONCLUSIONS: Our data suggests that basophils from asthmatic children are characterized by a specific increase in IgE mediated histamine releasability. The difference of histamine releasability with anti-IgE between atopic and nonatopic asthmatic children may be due to the heterogeneity of IgE bound to cell surface, or may be due to the degree of the basophil activation by cytokines such as IL3. The specific release of histamine with D. pteronyssinus antigen in atopic asthmatic group suggests that the basophil histamine release test can be used to diagnose the causing antigen.
Asthma ; Basophils* ; Calcimycin ; Calcium ; Child* ; Cytokines ; Histamine Release ; Histamine* ; Humans ; Immunoglobulin E ; Leukocytes ; Population Characteristics ; Suspensions