No abstract available.
Basophils* ; Histamine Release* ; Histamine*

No abstract available.
Animals ; Histamine Release* ; Histamine* ; Mast Cells* ; Rats*

No abstract available.
Anaphylaxis* ; Histamine Release* ; Histamine* ; Mast Cells*

No abstract available.
Animals ; Guinea Pigs* ; Guinea* ; Heart* ; Histamine Release* ; Histamine*

BACKGROUND: The fruit of Rubus coreanus (RC), a perennial herb, has been cultivated for a long time as a popular vegetable. The anti-allergy mechanism of RC is unknown. The purpose of this study is to investigate the inhibitory effect of RC on compound 48/80- or anti-DNP IgE-induced mast cell activation. METHODS: For this, influences of RC on the compound 48/80-induced degranulation, histamine release, calcium influx and the change of the intracellular cAMP (cyclic adenosine-3',5' monophosphate) levels of rat peritoneal mast cells (RPMC) and on the anti-DNP IgE-induced histamine release of RPMC were observed. RESULTS: The pretreatment of RC inhibited compound 48/ 80-induced degranulation, histamine release and intracelluar calcium uptake of RPMC. The anti-DNP IgE-induced histamine release of RPMC was significantly inhibited by pretreatment of RC. The RC increased the level of intracellular cAMP of RPMC, and the pretreatment of RC inhibited compound 48/80-induced decrement of intracellular cAMP of RPMC. CONCLUSION: These results suggest that RC contains some substances with an activity to inhibit the compound 48/80- or anti-DNP IgE-induced mast cell activitation. The inhibitory effects of RC are likely due to the stabilization of mast cells by blocking the calcium uptake and enhancing the level of intracellular cAMP.
Animals ; Calcium ; Fruit ; Histamine ; Histamine Release ; Mast Cells* ; Rats ; Vegetables

No abstract available.
Animals ; Calcium* ; Histamine Release* ; Histamine* ; Mast Cells* ; Rats*

No abstract available.
Aeromonas hydrophila* ; Aeromonas* ; Animals ; Histamine Release* ; Histamine* ; Mast Cells* ; Rats*

We previously described a rare case of anaphylaxis presumably induced by carminic acid in cochineal dye used as a food additive. In this study, highly pure carminic acid was added to an albumin-containing buffer at various concentrations, followed by serial dilution. Varying the mixing ratio of carminic acid and albumin affected the extent of histamine release from passively sensitized basophils. Similar basophil histamine release occurred with carminic acid-globulin solutions. These results provide experimental evidence indicating that basophil activation is dependent on hapten (carminic acid) and carrier (protein) interaction.
Anaphylaxis ; Basophils ; Carmine ; Food Additives ; Histamine Release ; Hypersensitivity ; In Vitro Techniques

BACKGROUND: Although there is evidence to support histamine involvement in atopic dermatitis, the role of histamine in pathogenesis of atopic dermatitis and the development of pruritus have been discussed controversially. OBJECTIVE: The purposes of this study were to investigate the plasma histamine levels in patients with atopic dermatitis of different severity and to evaluate the correlation between plasma histamine levels and other parameters of atopy, such as, basophil counts, eosinophil counts, and total IgE levels. METHODS: We measured plasma histamine levels, serum total IgE levels, peripheral basophil counts, and peripheral eosinophil counts in twenty patients with atopic dermatitis and in seventeen normal control subjects. RESULTS: 1. There was no significant difference in plasma histamine levels between patients with atopic dermatitis and normal control subjects. 2. A comparison of atopic dermatitis patients with increased plasma histamine levels and those with normal histamine levels revealed no difference regarding age, sex, and serum IgE levels; however, the group with increased plasma histamine levels was associated with other atopic disease, more commonly. 3. There were no correlation between the clinical severity of atopic dermatitis, seveirty of itching in patients with atopic dermatitis and the plasma histamine levels. 4. Plasma histamine levels in atopic dermatitis were correlated with basophil counts and eosinophil counts. However, there was no correlation between the plasma histamine levels and the serum total IgE levels. CONCLUSION: There was no significant difference of plasma histamine levels between patients with atopic dermatitis and normal control subjects. However, further investigations for measurement of histamine level after stimulation with variety of different substance may lead to clarification of evidence for histamine release in atopic dermatitis.
Basophils ; Dermatitis, Atopic* ; Eosinophils ; Histamine Release ; Histamine* ; Humans ; Immunoglobulin E ; Plasma* ; Pruritus

BACKGROUND: Our previous data suggested that IgE-mediated histamine release from basophil was elevated in the atopic asthmatic children. Basophil may play an important role in the development of the IgE-dependent, late phase response in allergen induced airway disease. So the existence of enhanced basophil histamine release in asthma could promote airway reactivity and obstruction. PURPOSE: The purpose of this study is to determine the relationship between IgE-mediated basophil histamine releasability (BHR) and airway hyperresponsiveness or markers of atopy in atopic children. METHODS: Twelve atopic asthmatics and four healthy atopics who were sensitive to D.p and D.f were selected. Their median age was 11.2 years old, their mean serum IgE level was 897+/-276 IU/mL and mean total eosinophil count was 536+/-71/mm3. Total eosinophil counts, total IgE, D.p and D.f-specific IgE, pulmonary function test, and methacholine provocation test were performed. IgE-mediated basophil histamine release by D.f allergen and goat-antihuman IgE antibody were measured by automated fluorometric assay. The relationship between histamine release and airway hyperresponsiveness or atopic markers was investigated. RESULTS: PC20 inversely correlated with anti-IgE antibody-mediated BHR (r=-0.50, P<0.05). Serum total IgE concentration correlated with anti-IgE antibody-mediated BHR (r=0.54, P<0.05). Serum concentrations of specific IgE to D.p correlated with anti-IgE antibody-mediated BHR (r=0.66, P<0.05). PC20 correlated correlated with FEF25-75% (r=0.75, P<0.05) and inversely with the total eosinophil counts (r=-0.69, P< 0.01). CONCLUSION: IgE-mediated basophil histamine releasability is inversely correlated with airway hyperresponsiveness, and correlated with total or specific-IgE in atopic children. These findings suggest that basophil histamine releasability is easy and useful method of diagnosis and monitoring response to treatment in atopic disease.
Asthma ; Basophils* ; Child* ; Diagnosis ; Eosinophils ; Histamine Release ; Histamine* ; Humans ; Immunoglobulin E ; Methacholine Chloride ; Respiratory Function Tests