No abstract available.
Genetic Markers* ; Immunity, Cellular*

When we intend to do the personal identification using DNA profiles, it will obviously be better to use as much information as possible. The power of identification is increased by using the genetic marker system such as VNTRs or STRs. Although STRs do not have many alleles per locus as VNTRs, these can be compensated by a large number of loci that are potentially usable. However, it will be more efficient to use a morphic loci. Therefore, prior to choose the genetic marker system of STRs for identification, it is essential to consider the statistical parameters of each STR locus, such as obs-H(observed heterozygosity), exp-H(expected heterozygosity), pM(probability of match), DI(discrimination index), PD(power of discrimination), MEC(mean exclusion chance), MEP(mean exclusion paternity), PIC(polymorphic information content) et al. In this article, we described the exact meaning of statistical parameters for the purpose of identification.
Alleles ; DNA ; Genetic Markers ; Humans

Pla sequence composes of 480 nucleodides of pPCP1 plasmide originated from Yersinia pestis isolated from Viet Nam and collected by the technique of PCR of chemical line into TA vector and demonstrated in turn. PCR reaction gave specific result from 3 diversified sources for mould: total extracted ADN, ADN extracted from plasmide isolated from bacterium complete cell. A fast and accurate method of diagnosis was created basing on this operation. By BLAST programme for accessing Gene Bank, the pla-gene sequence of Yersinia pestis in Viet Nam is analogue to other strains worldwide
Yersinia pestis ; Genetic Markers ; Biochemistry

OBJECTIVEStudies on DNA fingerprinting of eight species of Paris and application of restriction site amplification polymorphism (RSAP) to the identification of Paris.

METHODSequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity of 7 accessions of Paris collected from Tianquan and Baoxing in Sichuan, and one from Lijiang in Yunnan.

RESULTThe DNA fingerprinting of 8 species were generated by 18 primer combination screened from 45 primer combinations. Eight accessions were clustered into 4 groups by genetic distance.

CONCLUSIONBased on molecular biology methods of RSAP analysis, accurate molecular identification could be performed on traditional Chinese medicinal material plants in Paris, and provided molecular evidence for taxonomy and identification of different species in Paris.

Based on the current shortage of genuine/false authentication and quality evaluation in the molecular identification, and the weak functional gene research in the establishment of two-dimensional molecular markering methods for Chinese materia medica, the authors proposed a new method, the bimolecular marking methods (BIMM) for Chinese materia medica, combining DNA marker and metabolomics marker, that could simultaneously research the species and quality differences at the molecular level at the present stage. The authors introduced the concept, principle, methods, and technical process of BIMM, and summarized the technical advantages in this paper. Meanwhile, the application of BIMM in the identification of multiple sources of Chinese materia medica, years-identification, different locations, elite germplasm research, discovery of new drugs resources, protection of new varieties was also discussed. As a supplement of two-dimensional molecular markering method for Chinese materia medica, BIMM would not only expand connotation of identification of Chinese materia medica but also provide another effective way for quality evaluating.
Genetic Markers ; Materia Medica ; Medicine, Chinese Traditional

Infertility is known to be associated with chromosomal aberrations. Here the author reviews hitherto yet published cases of infertility identified to be carriers of small supernumerary marker chromosomes (sSMC). According to the sSMC web page (http://ssmc-tl. com/Start.html) there are now 225 cases of sSMC detected and characterized for their chromosomal origin and genetic content in infertile but otherwise health persons. In 54% of the cases, sSMC originated from chromosome 15 or 14, and was parentally transmitted in over 50% of the infertile sSMC-carriers. To the best of the authors knowledge, this is the largest review of infertile sSMC-carriers ever done.
Chromosome Aberrations ; Genetic Markers ; Humans ; Infertility ; genetics

Complex kinship analysis refers to a kind of special kinship analysis taken for the purpose of personal identification or other issues in civil or criminal cases because the father or （and） mother is dead, or cannot participate in the analysis for other reasons. Due to the absence of significant appraised persons in this kind of kinship analysis, grandparents, siblings or collateral relatives are required to participate in the analysis. Complex kinship analysis is widely used and the demand is increasing year by year. This paper analyzes the main types of complex kinships, the genetic markers of complex kinship analysis and their advantages and disadvantages and the calculation methods for complex kinship analysis by referring to the relevant literatures at home and abroad in recent years. At the same time, the shortcomings of the present research on complex kinship and its future development are prospected.
Genetic Markers ; Humans ; Pedigree ; Research ; Siblings

Objective To assess the feasibility of the rbcL sequence of chloroplast DNA as a genetic marker to identify Cannabis sativa L. Methods The rbcL sequences in 62 Cannabis sativa L. samples, 10 Humulus lupulus samples and 10 Humulus scandens DNA samples were detected, and 96 rbcL sequences of the Cannabaceae family were downloaded from Genbank. Sequence alignment was performed by MEGA X software, the intraspecific and interspecific Kimura-2-Parameter （K2P） genetic distances were calculated, and the system clustering tree was constructed. Results The rbcL sequence length acquired by sequencing of Cannabis sativa L. and Humulus scandens were 617 bp and 649 bp, respectively, and two haplotypes of Cannabis sativa L. were observed in the samples. The BLAST similarity search results showed that the highest similarity between the sequences acquired by sequencing and Cannabis sativa L. rbcL sequences available from Genbank was 100%. The genetic distance analysis showed that the maximum intraspecific genetic distance （0.004 9） of Cannabis sativa L. was less than the minimum interspecific genetic distance （0.012 9）. The results of median-joining network and system clustering tree analysis showed that Cannabis sativa L. and other members of the Cannabaceae family were located in different branches. Conclusion The rbcL sequence could be used as a DNA barcode for identifying Cannabis sativa L., and combined with comparative analysis of the rbcL sequence and system cluster analysis could be a reliable and effective detection method for Cannabis sativa L. identification in forensic investigation.
Cannabis/genetics* ; Genetic Markers ; Sequence Analysis, DNA

OBJECTIVETo determine the interspecies relationships of 18 Pueraria thomsonii cultivars in molecular level.

METHODEighteen P. thomsonii cultivars were evaluated by using sequence-related amplified polymorphism (SRAP) markers, with P. lobata and P. peduncularis as contrast species. Systematic relationships were constructed based on the UPGMA method by TREECONW software.

RESULTThe results showed that 22 primer pairs produced 338 loci, out of which 216 were polymorphic, the percentage of polymorphic loci was 63.9%. An average of 15.4 loci and 9.8 polymorphic loci were generated by each pair of primers. Genetic distance was analyzed by TREECONW software. Genetic distance of 18 P. thomsonii were changed from 0.004 7 to 0.265 8, with an average of 0.316. Using cluster analysis (UPGMA) based on those polymorphism bands amplified with SRAP primers, the 22 cultivars were classified into three groups, groups 1 with 18 P. thomsonii, group 2 with 3 P. lobata, and group 3 with 1 P. peduncularis. Most of the P. thomsonii from the same region were not in the same group.

CONCLUSIONSRAP markers could be a good marker for genetic relationship research in the P. thomsonii.

Chromosome Y does not recombine with any other at meiosis except that on pseudoautosomal region. Polymorphic markers on the chromosome Y are paternal inheritance and are haploidly inherited. Variance of the sequences comes from accumulated mutation. These properties make them unique and important not only to anthroponomy and genetics but also to forensic science and medicine.
Forensic Medicine ; Genetic Markers ; Humans ; Polymorphism, Genetic ; Y Chromosome