No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay* ; Korea*

No abstract available.
Autopsy* ; Enzyme-Linked Immunosorbent Assay*

BACKGROUND: The aim of our study was to optimize and establish erythropoietin (EPO) enzyme linked immunosorbent assay (ELISA) system. METHODS: We prepared several monoclonal and polyclonal antibodies specific to human-EPO. The best combinations of antibodies for coating and detecting antibodies were selected for the establishment of ELISA. We tested several methods such as a competitive EIA and a sandwich ELISA. RESULTS: The best sandwich ELISA was optimized compared to competitive EIA when purified polyclonal antibody (PoAb) was used as a coating antibody and biotinylated PoAb as a detecting antibody. This sandwich ELISA easily detected EPO when PoAb pairs were used compared to the ELISA using monoclonal antibody and PoAb. There were no significant differences between the effects of various blocking solutions on the performance of sandwich ELISA using biotinylated antibody. The ELISA system using PBST containing 3% BSA as a blocking solution can sensitively detect EPO (10 mU/mL) in a broad range of EPO concentrations (10-2,000 mU/mL) and there were cross-reactions with other cytokines). CONCLUSIONS: EPO can be easily determined by using biotinylated PoAb as a detecting antibody and another PoAb as a coating antibody.
Antibodies ; Enzyme-Linked Immunosorbent Assay* ; Erythropoietin*

In the year 2003, for Hantaan virus, a serological surveillance ELISA method was performed on 155 serum samples collected from the workers of Haiphong port.26/155 samples were positive or in suspect with rodent sera. The samples submitted to 2nd examen by indirect fluoresceine immuno-assay showed that 3/26 samples were positive 3 positive samples were undertaken further studies by WB technique with specific SEO antigenes ,which confirmed anti-hantaan virus (IgG) and nuclecapsid protein (NP) of51 kD weigt. Results showed that Hantaan virus was found among Haiphong port workers
Hantavirus ; Serum ; Enzyme-Linked Immunosorbent Assay

Healthy human serum IgA, purified lectin-sepharose 4B column affinity chromatography was assessed by sodium dodecyl sulfate polyacrylamide electrophresis. The lectin from wild Jackfruit reacted specifically to IgA1 from human serum. This lectin used as antigen in the technique of ELISA-Lectin to determine the concentration of IgA1 from healthy and pathological human sera. Using protein A from Staphylococcus aureus isolated from Vietnamese infectious patients to capture IgG average concentration of IgA1 from healthy human serum as 14.37 mg/ml. No change of IgG concentration from patients sera (hepatoma and leukemia) was obtained in comparison with healthy subjects
Carcinoma, Hepatocellular ; Enzyme-Linked Immunosorbent Assay ; Leukemia ;

On 138 serum samples taken in the first 7 days of acute syndrome of brain, by MAC-ELISA technique 95 samples were determined as positive, 68.84% was positive. 43 samples taken in the first 7 days of the disease there was no detected antivirus IgM antibody but this could not concluded as negative, but may be a late antibody response or may be another cause of acute brain syndrome. For assuring the accuracy of the diagnosis, the samples taken in the first 7 days of the disease must be taken again after 7 day to verify the results.
Encephalitis, Japanese ; Diagnosis ; Enzyme-Linked Immunosorbent Assay

In Trang An polyclinics in Ha Noi, with ELISA technique, 35 healthy people (27 female aged 20-38, 8 male aged 25-37) undergone an examination of serum CA153-3 and 31 other healthy people (17 female aged 21-38, 14 male aged 22-41) an examination of resum CA125. The technique is complicated needing precaution and accuracy; for each examination, it is recommended a standard graphic with a standard sample determined antigen concentration. CA15-3 normal concentration is 2.37-18.17 U/ml, and CA125 is 7.17-38.37 U/ml
Enzyme-Linked Immunosorbent Assay ; methods ; serum