1.Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappa B pathway in human dermal fibroblasts.
Young Rae LEE ; Eun Mi NOH ; Eun Yong JEONG ; Seok Kweon YUN ; Young Ju JEONG ; Jong Hyeon KIM ; Kang Beom KWON ; Byeong Soo KIM ; Sung Ho LEE ; Chang Sik PARK ; Jong Suk KIM
Experimental & Molecular Medicine 2009;41(8):548-554
Cordycepin (3'-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappa B activity, which was determined by I kappa B alpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappa B binding activity. However, UVB-induced NF-kappa B activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappa B activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.
Aging/physiology
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Cells, Cultured
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Deoxyadenosines/*pharmacology
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*Dermis/cytology/drug effects/physiology/radiation effects
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Dose-Response Relationship, Drug
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Enzyme Induction/drug effects
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Fibroblasts/drug effects/metabolism/radiation effects
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Gene Expression Regulation, Enzymologic
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Humans
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Infant, Newborn
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Male
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*Matrix Metalloproteinase 1/antagonists & inhibitors/biosynthesis/genetics/radiation effects
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Matrix Metalloproteinase 3/antagonists & inhibitors/*biosynthesis/genetics/radiation effects
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NF-kappa B/*antagonists & inhibitors/genetics/metabolism
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Skin/physiopathology/radiation effects
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*Ultraviolet Rays