1.Characterization and clinical application of mesenchymal stem cells from equine umbilical cord blood.
Jun Gu KANG ; Sang Bum PARK ; Min Soo SEO ; Hyung Sik KIM ; Joon Seok CHAE ; Kyung Sun KANG
Journal of Veterinary Science 2013;14(3):367-371
Tendinitis of the superficial digital flexor tendon (SDFT) is a significant cause of lameness in horses; however, recent studies have shown that stem cells could be useful in veterinary regenerative medicine. Therefore, we isolated and characterized equine umbilical cord blood mesenchymal stem cells (eUCB-MSCs) from equine umbilical cord blood obtained from thoroughbred mares during the foaling period. Horses that had tendinitis of the SDFT were treated with eUCB-MSCs to confirm the therapeutic effect. After eUCB-MSCs transplantation, the core lesion in the SDFT was found to decrease. These results suggest that transplantation using eUCB-MSCs could be another source of cell treatment.
Animals
;
*Cord Blood Stem Cell Transplantation/veterinary
;
Horse Diseases/*surgery
;
Horses
;
Male
;
Tendinopathy/surgery/*veterinary
2.Percutaneous transplantation of human umbilical cord-derived mesenchymal stem cells in a dog suspected to have fibrocartilaginous embolic myelopathy.
Wook Hun CHUNG ; Seon Ah PARK ; Jae Hoon LEE ; Dai Jung CHUNG ; Wo Jong YANG ; Eun Hee KANG ; Chi Bong CHOI ; Hwa Seok CHANG ; Dae Hyun KIM ; Soo Han HWANG ; Hoon HAN ; Hwi Yool KIM
Journal of Veterinary Science 2013;14(4):495-497
The use of human umbilical cord blood-derived mesenchymal stem cells for cell transplantation therapy holds great promise for repairing spinal cord injury. Here we report the first clinical trial transplantation of human umbilical cord (hUCB)-derived mesenchymal stem cells (MSCs) into the spinal cord of a dog suspected to have fibrocartilaginous embolic myelopathy (FCEM) and that experienced a loss of deep pain sensation. Locomotor functions improved following transplantation in a dog. Based on our findings, we suggest that transplantation of hUCB-derived MSCs will have beneficial therapeutic effects on FCEM patients lacking deep pain sensation.
Animals
;
Cartilage Diseases/etiology/therapy/*veterinary
;
*Cord Blood Stem Cell Transplantation/veterinary
;
Dog Diseases/etiology/*therapy
;
Dogs
;
Embolism/etiology/therapy/*veterinary
;
Female
;
Humans
;
Mesenchymal Stromal Cells/cytology/*metabolism
;
Spinal Cord Diseases/etiology/therapy/*veterinary
;
Treatment Outcome
3.Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs.
Ji Hey LIM ; Ye Eun BYEON ; Hak Hyun RYU ; Yun Hyeok JEONG ; Young Won LEE ; Wan Hee KIM ; Kyung Sun KANG ; Oh Kyeong KWEON
Journal of Veterinary Science 2007;8(3):275-282
This study was to determine the effects of allogenicumbilical cord blood (UCB)-derived mesenchymal stemcells (MSCs) and recombinant methionyl humangranulocyte colony-stimulating factor (rmhGCSF) on acanine spinal cord injury model after balloon compressionat the first lumbar vertebra. Twenty-five adult mongreldogs were assigned to five groups according to treatmentafter a spinal cord injury: no treatment (CN); salinetreatment (CP); rmhGCSF treatment (G); UCB-MSCstreatment (UCB-MSC); co-treatment (UCBG). The UCB-MSCs isolated from cord blood of canine fetuses wereprepared as 10(6) cells/150microl saline. The UCB-MSCs weredirectly injected into the injured site of the spinal cord andrmhGCSF was administered subcutaneously 1 week afterthe induction of spinal cord injury. The Olby score,magnetic resonance imaging, somatosensory evokedpotentials and histopathological examinations were used toevaluate the functional recovery after transplantation. TheOlby scores of all groups were zero at the 0-week evaluation.At 2 week after the transplantation, the Olby scores in thegroups with the UCB-MSC and UCBG were significantlyhigher than in the CN and CP groups. However, there wereno significant differences between the UCB-MSC andUCBG groups, and between the CN and CP groups. Thesecomparisons remained stable at 4 and 8 week aftertransplantation. There was significant improvement in thenerve conduction velocity based on the somatosensory evokedpotentials. In addition, a distinct structural consistency ofthe nerve cell bodies was noted in the lesion of the spinalcord of the UCB-MSC and UCBG groups. These resultssuggest that transplantation of the UCB-MSCs resulted inrecovery of nerve function in dogs with a spinal cord injuryand may be considered as a therapeutic modality for spinalcord injury.
Animals
;
Behavior, Animal/physiology
;
Cord Blood Stem Cell Transplantation/methods/*veterinary
;
Dog Diseases/pathology/*therapy
;
Dogs
;
Evoked Potentials, Somatosensory/physiology
;
Histocytochemistry/veterinary
;
Magnetic Resonance Imaging/veterinary
;
Random Allocation
;
Spinal Cord Injuries/pathology/therapy/*veterinary
;
Videotape Recording