1.Human Cloning, Is It a Victory of Science?.
Journal of the Korean Medical Association 2003;46(4):266-267
No abstract available.
Cloning, Organism*
2.Human Cloning: Hope or Disaster.
Journal of the Korean Medical Association 2003;46(4):264-265
No abstract available.
Cloning, Organism*
;
Disasters*
;
Hope*
3.Human Cloning.
Journal of the Korean Medical Association 1999;42(4):369-374
No abstract available.
Cloning, Organism*
;
Humans*
4.Cloning of HEV recombinant protein expressed in Pichia pastoris
Journal of Preventive Medicine 2000;10(4):46-51
The fragment in ORF2 of HEV genome, which is coded for structural protein was transformed into Pichia pastoris GS115 strain (Mut+) after confirmation of positives clones by PCR and DNA sequencing. After induction by methanol for 4 days, shaking at 30oC, the cells were collected and disrupted. The purification of recombinant protein was checked by SDS-PAGE and the presence of HEV recombinant antigen was identified by Western Blot. High antigenicity of HEV recombinant protein was constructed
Cloning, Organism
;
Proteins
5.Location and cloning of the polyhedrin gene of hyphantria cunea nuclear polyhedrosis virus.
Hyung Hoan LEE ; Mi Kyung LEE ; Il Hwan CHO ; Kwan Hee YOO
Journal of the Korean Society of Virology 1991;21(1):25-34
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Nucleopolyhedrovirus*
6.Cloning and map location of thymidine kinase(TK) gene of Korean isolate bovine herpesvirus PQ strain.
Chang Hee KWEON ; Young Jin KEE ; Byung Joon KWON ; Soo Hwan AN
Journal of the Korean Society of Virology 1993;23(2):165-169
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Thymidine*
7.Cloning and protein expression of Actinobacillus actinomycetemcomitans cytolethal distending toxin subunit CdtA.
Sun Young KO ; Dong Keun JEONG ; So Hyun RYU ; Hyung Seop KIM
The Journal of the Korean Academy of Periodontology 2007;37(Suppl):339-351
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Clone Cells*
;
Cloning, Organism*
8.Cloning and Expression of Low Molecule Protein Antigens from Mycobacterium tuberculosis H37Rv.
Jae Hyun LIM ; Hwa Jung KIM ; Eun Kueong JO ; Chang Hwa SONG ; Un Ok KIM ; Jeong Kyu PARK
Journal of the Korean Society for Microbiology 2000;35(5):375-375
No Abstract Available.
Clone Cells*
;
Cloning, Organism*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
9.Cloning of the Form I-antigen Genes from Shigella sonnei KNIH104.
Hyoung Kyun OH ; Young Chang KIM ; Jai Yun LIM ; Na Gyong LEE ; Yong Soo BAE ; Kwang Jun LEE
Journal of the Korean Society for Microbiology 1997;32(2):161-166
Shigella sonnei KNIH104S, which was selected by Korean National Institute of Health, expresses form I-antigen as a somatic antigen. In this study, we cloned the genes responsible for form I-antigen synthesis from S. sonnei KNIH104S. A Sau3AI-generated cosmid library of S. sonnei KNIH104S plasmids were transfected into E. coli LE392 and transfectants were tested for agglutination with antiserum against S. sonnei form I-antigen. A clone, JH222, showing the strongest agglutination activity was chosen for further analysis. A recombinant cosmid, pJH222, was isolated from the strain JH222 and retransfected into E. coli LE392. All of the transfectants agglutinated with antiserum against form I-antigen, indicating that pJH222 carried the genes required for S. sonnei form I-antigen synthesis. Restriction analysis of pJH222 revealed a 38 kb insert, which was confirmed by Southern hybridization analysis to be present on a large plasmid of S. sonnei KNIH104S.
Agglutination
;
Clone Cells*
;
Cloning, Organism*
;
Cosmids
;
Plasmids
;
Shigella sonnei*
;
Shigella*
10.Cloning of Mouse Pleckstrin 1 from Mast Cell Subtraction Library.
Young Baik HAM ; Hyun Jong AHN ; Youn Mun HA ; Jeong Je CHO
Korean Journal of Immunology 2000;22(4):265-273
No abstract available.
Animals
;
Clone Cells*
;
Cloning, Organism*
;
Mast Cells*
;
Mice*
Result Analysis
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