1.Roles of AKR1C3 in malignancy.
Xin-Zhu XIAO ; Li-Ying LIN ; Ming-Kai ZHUANG ; Can-Mei ZHONG ; Feng-Lin CHEN
Chinese Medical Journal 2021;134(9):1052-1054
2.HPCAL1 is a novel driver of autophagy-dependent ferroptosis.
Liwen WANG ; Qin LI ; Huimei LIU ; Lanfang LI
Journal of Zhejiang University. Science. B 2023;24(11):1053-1056
自噬是细胞内一种高度保守的生理过程,可通过溶酶体系统降解过量或受损的细胞器、有毒的蛋白聚集体和病原体等。最新研究表明,海马钙素样1(HPCAL1)可作为特异性自噬受体和铁死亡的正调节因子。HPCAL1可选择性降解钙粘素2(CDH2),加速脂质过氧化,促进癌细胞铁死亡。iHPCAL1是抑制HPCAL1的小分子化合物,可抑制Erastin诱导的肿瘤细胞铁死亡。此外,它还可以抑制铁死亡诱导的急性胰腺炎。本文通过对HPCAL1在铁死亡中的具体作用机制进行概述,为HPCAL1作为铁死亡相关疾病的潜在治疗靶点提供新思路和理论依据。
Ferroptosis
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Cell Line, Tumor
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Autophagy
4.Immunologic effects of recombinant gamma-interferon on human gastrointestinal tumor cell lines.
Doo Hyun CHUNG ; Young Mi BAE ; Jae Gahb PARK ; Sung Hoe PARK ; Yong Il KIM ; Sang Kook LEE
Journal of the Korean Cancer Association 1991;23(1):10-19
No abstract available.
Cell Line, Tumor*
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Humans*
;
Interferon-gamma*
5.Compounds from a jellyfish-derived fungus Aspergillus fumigates.
Guan Yu TAO ; Juan LIU ; Jee H JUNG ; Wei GUO ; Xiao Qiong WEN ; Yonghong LIU
Natural Product Sciences 2016;22(2):82-86
Six compounds were isolated from the secondary metabolites of the jellyfish-derived fungus Aspergillus fumigates, whose structures were identified by chemical methods and spectroscopic analysis as pseurotin F1 (1), azaspirofurans B (2), (22E, 24R)-24-methyl-5α-cholesta-7,22-diene-3β,5,6β-triol (3), 5α,8α-epidioxyergosta-6,22-dien-3β-o1 (4), cyclo-(L-Pro-L-Tyr) (5), fumitremorgin C (6). The compounds 1 - 5 were isolated from the fungus Aspergillus fumigates for the first time. The isolated compounds (1 - 6) were evaluated for antibiotic activity and cytotoxicity against six bacterial strains and ten human tumor cell lines, respectively.
Aspergillus*
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Cell Line, Tumor
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Fungi*
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Humans
6.Antitumor activity of adriamycin and the analogue, THP-adriamycin and epirubicin, against human tumor cell lines.
Weon Seon HONG ; Chang Min KIM ; Myung Shick LEE ; Yoon Koo KANG ; Choon Taek LEE ; You Cheoul KIM ; Jhin Oh LEE ; Tae Woong KANG
Journal of the Korean Cancer Association 1991;23(2):259-265
No abstract available.
Cell Line, Tumor*
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Doxorubicin*
;
Epirubicin*
;
Humans*
8.Cembranoids and their bioactivities in soft coral Sarcophyton glaucum.
Min WU ; Kai-Bing ZHOU ; Hao-Fu DAI ; Yan-Bo ZENG
China Journal of Chinese Materia Medica 2023;48(3):707-714
Chemical constituents in soft coral Sarcophyton glaucum were separated and purified by various chromatographic methods. Based on the spectral data, physicochemical properties, and comparison with the data reported in the literature, nine cembranoids, including a new cembranoid named sefsarcophinolide(1) together with eight known cembranoids, namely(+)-isosarcophine(2), sarcomilitatin D(3), sarcophytonolide J(4),(1S,3E,7E,13S)-11,12-epoxycembra-3,7,15-triene-13-ol(5), sarcophytonin B(6),(-)-eunicenone(7), lobophytin B(8), and arbolide C(9), were identified. As revealed by biological activity experiment results, compounds 2-6 had weak acetylcholinesterase inhibitory activity, and compound 5 displayed weak cytotoxicity against K562 tumor cell line.
Animals
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Anthozoa
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Acetylcholinesterase
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Cell Line, Tumor
9.Establishment of subseries cell lines from tongue cancer single cell and detection of cancer stem cell markers.
Jinguang YAO ; Jisheng XIE ; Jun LI ; Xing WEI ; Yongrong YANG ; Haibo CHEN
West China Journal of Stomatology 2013;31(1):86-90
OBJECTIVETo establish subseries cell lines from single, cancer cell of Tca8113M1 cell line and detect the cancer stem cell markers in the different subseries cell lines.
METHODSThe subseries cell lines from single cancer cell of Tca8113M1 cell line were established by limiting dilution assay in vitro. The characteristic of tumorigenicity and CD44, CD184, extracellular soluble antigen (ESA) of the cancer stem cell markers were detected by xenotransplantation and flow cytometry respectively.
RESULTSTotal 192 single cells of Tca8113M1 cell line were cultured and were deposited as one cell per well. There were 12 subpopulations origin from 192 single cells spheroid cultures. The ratio was 6.25% (12/192). In the different subpopulations, the tumorigenicity and expression of CD44 and ESA were at high levels, but the expression of CD184 was in different level. There were three kinds morphology of colonies derived from single cancer cells, holoclone, meroclone and paraclone. Cell line could be derived from carcinoma cell holoclones by cell culture. Meroclone and paraclone did not exist in cell culture in vitro.
CONCLUSIONTongue cancer stem cell may exist in Tca8113M1 cell line, cell line can be established and holoclone is the origin of cell line. This is a novel approach to the identification and enrichment for cancer stem cell.
Biomarkers, Tumor ; Cell Line ; Cell Line, Tumor ; Flow Cytometry ; Humans ; Neoplastic Stem Cells ; Tongue Neoplasms
10.The effect of oral carcinoma-associated fibroblasts on the invasion of carcinoma cell line.
Jing-wen LIN ; Hong-mei ZHOU ; Sheng-fu LI ; Tao HU ; Qian-ming CHEN
West China Journal of Stomatology 2006;24(2):101-103
OBJECTIVETo observe the effect of oral carcinoma-associated fibroblasts (CAFs) on the invasion of a lingual carcinoma cell line, and to elucidate the role of CAFs in oral squamous cell carcinoma (OSCC) progression.
METHODSMatrigel was used to remodel the basement memberane, and the interaction model between primary oral CAFs and lingual carcinoma cell line Tca8113 was established by Transwell chamber to observe the effect of CAFs on the invasion of Tca8113.
RESULTSCompared with normal fibroblasts (NFs), CAFs promoted more Tca8113 cells to penetrate Matrigel (P < 0.05).
CONCLUSIONOral CAFs can promote the invasion of lingual carcinoma cell line Tca8113, and play a key role in OSCC progression.
Carcinoma, Squamous Cell ; Cell Line ; Cell Line, Tumor ; Cell Proliferation ; Fibroblasts ; Humans ; Mouth Neoplasms ; Tongue Neoplasms