1.Factors Influencing Alzheimer's Disease Risk: Whether and How They are Related to the APOE Genotype.
Rong ZHANG ; Xiaojiao XU ; Hang YU ; Xiaolan XU ; Manli WANG ; Weidong LE
Neuroscience Bulletin 2022;38(7):809-819
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease featuring progressive cognitive impairment. Although the etiology of late-onset AD remains unclear, the close association of AD with apolipoprotein E (APOE), a gene that mainly regulates lipid metabolism, has been firmly established and may shed light on the exploration of AD pathogenesis and therapy. However, various confounding factors interfere with the APOE-related AD risk, raising questions about our comprehension of the clinical findings concerning APOE. In this review, we summarize the most debated factors interacting with the APOE genotype and AD pathogenesis, depict the extent to which these factors relate to APOE-dependent AD risk, and discuss the possible underlying mechanisms.
Alzheimer Disease/pathology*
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Apolipoprotein E4/genetics*
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Apolipoproteins E/genetics*
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Genotype
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Humans
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Lipid Metabolism
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Neurodegenerative Diseases
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Risk Factors
2.Disease-specific induced pluripotent stem cells: a platform for human disease modeling and drug discovery.
Jiho JANG ; Jeong Eun YOO ; Jeong Ah LEE ; Dongjin R LEE ; Ji Young KIM ; Yong Jun HUH ; Dae Sung KIM ; Chul Yong PARK ; Dong Youn HWANG ; Han Soo KIM ; Hoon Chul KANG ; Dong Wook KIM
Experimental & Molecular Medicine 2012;44(3):202-213
The generation of disease-specific induced pluripotent stem cell (iPSC) lines from patients with incurable diseases is a promising approach for studying disease mechanisms and drug screening. Such innovation enables to obtain autologous cell sources in regenerative medicine. Herein, we report the generation and characterization of iPSCs from fibroblasts of patients with sporadic or familial diseases, including Parkinson's disease (PD), Alzheimer's disease (AD), juvenile-onset, type I diabetes mellitus (JDM), and Duchenne type muscular dystrophy (DMD), as well as from normal human fibroblasts (WT). As an example to modeling disease using disease-specific iPSCs, we also discuss the previously established childhood cerebral adrenoleukodystrophy (CCALD)- and adrenomyeloneuropathy (AMN)-iPSCs by our group. Through DNA fingerprinting analysis, the origins of generated disease-specific iPSC lines were identified. Each iPSC line exhibited an intense alkaline phosphatase activity, expression of pluripotent markers, and the potential to differentiate into all three embryonic germ layers: the ectoderm, endoderm, and mesoderm. Expression of endogenous pluripotent markers and downregulation of retrovirus-delivered transgenes [OCT4 (POU5F1), SOX2, KLF4, and c-MYC] were observed in the generated iPSCs. Collectively, our results demonstrated that disease-specific iPSC lines characteristically resembled hESC lines. Furthermore, we were able to differentiate PD-iPSCs, one of the disease-specific-iPSC lines we generated, into dopaminergic (DA) neurons, the cell type mostly affected by PD. These PD-specific DA neurons along with other examples of cell models derived from disease-specific iPSCs would provide a powerful platform for examining the pathophysiology of relevant diseases at the cellular and molecular levels and for developing new drugs and therapeutic regimens.
Alzheimer Disease/genetics/*pathology
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Cell Differentiation
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Cells, Cultured
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Diabetes Mellitus, Type 1/genetics/*pathology
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Drug Discovery/*methods
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Fibroblasts/cytology/metabolism/pathology
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Gene Expression
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Humans
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Induced Pluripotent Stem Cells/cytology/metabolism/*pathology
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Muscular Dystrophy, Duchenne/genetics/*pathology
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Parkinson Disease/genetics/*pathology
3.Cholesterol metabolism and homeostasis in the brain.
Protein & Cell 2015;6(4):254-264
Cholesterol is an essential component for neuronal physiology not only during development stage but also in the adult life. Cholesterol metabolism in brain is independent from that in peripheral tissues due to blood-brain barrier. The content of cholesterol in brain must be accurately maintained in order to keep brain function well. Defects in brain cholesterol metabolism has been shown to be implicated in neurodegenerative diseases, such as Alzheimer's disease (AD), Huntington's disease (HD), Parkinson's disease (PD), and some cognitive deficits typical of the old age. The brain contains large amount of cholesterol, but the cholesterol metabolism and its complex homeostasis regulation are currently poorly understood. This review will seek to integrate current knowledge about the brain cholesterol metabolism with molecular mechanisms.
ATP-Binding Cassette Transporters
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genetics
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metabolism
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Alzheimer Disease
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genetics
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metabolism
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pathology
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Blood-Brain Barrier
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Brain
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metabolism
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pathology
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Cholesterol
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metabolism
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Gene Expression Regulation
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Homeostasis
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Humans
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Huntington Disease
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genetics
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metabolism
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pathology
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Hydroxycholesterols
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metabolism
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Lipid Metabolism
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genetics
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Neurons
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metabolism
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pathology
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Parkinson Disease
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genetics
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metabolism
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pathology
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Receptors, Lipoprotein
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genetics
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metabolism
4.Analysis of differential plaque depositions in the brains of Tg2576 and Tg-APPswe/PS1dE9 transgenic mouse models of Alzheimer disease.
Tae Kyung KIM ; Jung Eun LEE ; Sun Kyu PARK ; Kang Woo LEE ; Ji Seon SEO ; Joo Young IM ; Sang Tae KIM ; Joo Yong LEE ; Yang Hee KIM ; Ja Kyeong LEE ; Pyung Lim HAN
Experimental & Molecular Medicine 2012;44(8):492-502
Adequate assessment of plaque deposition levels in the brain of mouse models of Alzheimer disease (AD) is required in many core issues of studies on AD, including studies on the mechanisms underlying plaque pathogenesis, identification of cellular factors modifying plaque pathology, and developments of anti-AD drugs. The present study was undertaken to quantitatively evaluate plaque deposition patterns in the brains of the two popular AD models, Tg2576 and Tg-APPswe/PS1dE9 mice. Coronally-cut brain sections of Tg2576 and Tg-APPswe/PS1dE9 mice were prepared and plaque depositions were visualized by staining with anti-amyloid beta peptides antibody. Microscopic images of plaque depositions in the prefrontal cortex, parietal cortex, piriform cortex and hippocampus were obtained and the number of plaques in each region was determined by a computer-aided image analysis method. A series of optical images representing a gradual increase of plaque deposition levels were selected in the four different brain regions and were assigned in each with a numerical grade of 1-6, where +1 was lowest and +6, highest, so that plaques per unit in mm2 increased "sigmoidally" over the grading scales. Analyzing plaque depositions using the photographic plaque reference panels and a computer-aid image analysis method, it was demonstrated that the brains of Tg2576 mice started to accumulate predominantly small plaques, while the brains of Tg-APPswe/PS1dE9 mice deposited relatively large plaques.
Alzheimer Disease/genetics/*pathology
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Amyloid beta-Protein Precursor/genetics/metabolism
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Animals
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Disease Models, Animal
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Humans
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Mice
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Mice, Transgenic
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Plaque, Amyloid/*pathology
5.Review of neuropathology in the past 10 years in China.
Chinese Journal of Pathology 2005;34(9):550-552
6.Increased expression of the receptor for advanced glycation end products in neurons and astrocytes in a triple transgenic mouse model of Alzheimer's disease.
Bo Ryoung CHOI ; Woo Hyun CHO ; Jiyoung KIM ; Hyong Joo LEE ; Chihye CHUNG ; Won Kyung JEON ; Jung Soo HAN
Experimental & Molecular Medicine 2014;46(2):e75-
The receptor for advanced glycation end products (RAGE) has been reported to have a pivotal role in the pathogenesis of Alzheimer's disease (AD). This study investigated RAGE levels in the hippocampus and cortex of a triple transgenic mouse model of AD (3xTg-AD) using western blotting and immunohistochemical double-labeling to assess cellular localization. Analysis of western blots showed that there were no differences in the hippocampal and cortical RAGE levels in 10-month-old adult 3xTg-AD mice, but significant increases in RAGE expression were found in the 22- to 24-month-old aged 3xTg-AD mice compared with those of age-matched controls. RAGE-positive immunoreactivity was observed primarily in neurons of aged 3xTg-AD mice with very little labeling in non-neuronal cells, with the notable exception of RAGE presence in astrocytes in the hippocampal area CA1. In addition, RAGE signals were co-localized with the intracellular amyloid precursor protein (APP)/amyloid beta (Abeta) but not with the extracellular APP/Abeta. In aged 3xTg-AD mice, expression of human tau was observed in the hippocampal area CA1 and co-localized with RAGE signals. The increased presence of RAGE in the 3xTg-AD animal model showing critical aspects of AD neuropathology indicates that RAGE may contribute to cellular dysfunction in the AD brain.
Advanced Glycosylation End Product-Specific Receptor
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Alzheimer Disease/genetics/*metabolism
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Amyloid beta-Peptides/metabolism
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Animals
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Astrocytes/*metabolism
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CA1 Region, Hippocampal/growth & development/metabolism/pathology
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Humans
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Mice
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Mice, Transgenic
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Neurons/*metabolism
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Receptors, Immunologic/genetics/*metabolism
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tau Proteins/genetics/metabolism
7.Expression of apolipoprotein E in Alzheimer's disease and its significance.
Shu-rong HE ; Dong-ge LIU ; Shu WANG ; Yong-jing XIA
Chinese Journal of Pathology 2005;34(9):556-560
OBJECTIVETo study the association between Alzheimer' s disease (AD) and apolipoprotein E (apoE) polymorphism and apoE epsilon4 allele; and to investigate the role of apoE in senile plaque formation.
METHODSDuring the period from 1982 to 2003, 27 portmortem cases of AD from the archival files of Department of Pathology of Beijing Hospital, diagnosed according to the consortium to establish a registry for Alzheimer's disease (CERAD) criteria, were enrolled into this study. Among the 27 cases studied, there were 23 cases of definite AD and 4 cases of probable AD. Postmortem brain tissues from 67 neurologically unremarkable deceased were used as age-matched controls. Immunohistochemical study for beta-amyloid (Abeta) and Tau protein, as well as immunohistochemical study for Abeta/apoE, were performed in all AD cases using streptavidin-peroxidase (SP) and double immunostaining ( SP/ABC) methods, respectively. Senile plaques and neurofibrillary tangles in the 23 cases of definite AD were further quantified. The apoE genotypes in all cases were analyzed by polymerase chain reaction and restriction fragment length polymorphism technologies.
RESULTSImmunohistochemical study for Abeta distinguished 4 different types of senile plaques: diffuse non-neuritic plaques, diffuse neuritic plaques, dense-core neuritic plaques and dense-core non-neuritic plaques. Double immunohistochemistry for Abeta/apoE showed that some senile plaques were positive for both Abeta and apoE. The expression rates for Abeta and apoE in these 4 different types of senile plaques were 4. 28%, 84. 71%, 8.50% and 2.51%, respectively. The positivity rate for Abeta/apoE in diffuse neuritic plaques were significantly higher than those in other 3 types (P < 0.01). The frequency of occurrence of apoE epsilon4 allele in AD was significantly higher than that in the control group (P < 0.01). The numbers of senile plaques and neurofibrillary tangles in AD cases with apoE epsilon4 allele were also significantly higher than those in AD cases without apoE epsilon4 allele (P < 0.01).
CONCLUSIONSApoE polymorphism is associated with AD. The presence of apoE epsilon4 allele carries a higher risk for the development of AD. ApoE may also play an important role in the transformation of diffuse non-neuritic plaques to diffuse neuritic plaques.
Aged ; Aged, 80 and over ; Alleles ; Alzheimer Disease ; metabolism ; pathology ; Amyloid beta-Peptides ; metabolism ; Apolipoproteins E ; genetics ; metabolism ; Brain ; metabolism ; pathology ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Neurofibrillary Tangles ; pathology ; Plaque, Amyloid ; pathology ; tau Proteins ; metabolism
8.Early Activation of Astrocytes does not Affect Amyloid Plaque Load in an Animal Model of Alzheimer's Disease.
Dongpi WANG ; Xiaoqin ZHANG ; Mingkai WANG ; Dongming ZHOU ; Hongyu PAN ; Qiang SHU ; Binggui SUN
Neuroscience Bulletin 2018;34(6):912-920
Astrocytes are closely associated with Alzheimer's disease (AD). However, their precise roles in AD pathogenesis remain controversial. One of the reasons behind the different results reported by different groups might be that astrocytes were targeted at different stages of disease progression. In this study, by crossing hAPP (human amyloid precursor protein)-J20 mice with a line of GFAP-TK mice, we found that astrocytes were activated specifically at an early stage of AD before the occurrence of amyloid plaques, while microglia were not affected by this crossing. Activation of astrocytes at the age of 3-5 months did not affect the proteolytic processing of hAPP and amyloid plaque loads in the brains of hAPP-J20 mice. Our data suggest that early activation of astrocytes does not affect the deposition of amyloid β in an animal model of AD.
Aldehyde Dehydrogenase
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metabolism
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Alzheimer Disease
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genetics
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metabolism
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pathology
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Amyloid beta-Peptides
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metabolism
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Amyloid beta-Protein Precursor
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genetics
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metabolism
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Animals
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Astrocytes
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metabolism
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Brain
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pathology
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Calcium-Binding Proteins
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metabolism
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Cell Proliferation
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Disease Models, Animal
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Gene Expression Regulation
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genetics
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Glial Fibrillary Acidic Protein
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Glutamine
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metabolism
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Green Fluorescent Proteins
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genetics
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metabolism
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Humans
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Ki-67 Antigen
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metabolism
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Mice
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Mice, Transgenic
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Microfilament Proteins
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metabolism
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Mutation
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genetics
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Nerve Tissue Proteins
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metabolism
9.Effects of TYROBP Deficiency on Neuroinflammation of a Alzheimer's Disease Mouse Model Carrying a PSEN1 p.G378E Mutation.
Li RAN ; Lv ZHAN-YUN ; Li YAN-XIN ; Li WEI ; Hao YAN-LEI
Chinese Medical Sciences Journal 2022;37(4):320-330
Objective To study the effects of TYRO protein kinase-binding protein (TYROBP) deficiency on learning behavior, glia activation and pro-inflammatory cycokines, and Tau phosphorylation of a new Alzheimer's disease (AD) mouse model carrying a PSEN1 p.G378E mutation.Methods A new AD mouse model carrying PSEN1 p.G378E mutation was built based on our previously found AD family which might be ascribed to the PSEN1 mutation, and then crossed with TYROBP deficient mice to produce the heterozygous hybrid mice (PSEN1G378E/WT; Tyrobp+/-) and the homozygous hybrid mice (PSEN1G378E/G378E; Tyrobp-/-). Water maze test was used to detect spatial learning and memory ability of mice. After the mice were sacrificed, the hippocampus was excised for further analysis. Immunofluorescence was used to identify the cell that expresses TYROBP and the number of microglia and astrocyte. Western blot was used to detect the expression levels of Tau and phosphorylated Tau (p-Tau), and ELISA to measure the levels of pro-inflammatory cytokines. Results Our results showed that TYROBP specifically expressed in the microglia of mouse hippocampus. Absence of TYROBP in PSEN1G378E mutation mouse model prevented the deterioration of learning behavior, decreased the numbers of microglia and astrocytes, and the levels of interleukin-6, interleukin-1β and tumor necrosis factor-α in the hippocampus (all P < 0.05). The ratios of AT8/Tau5, PHF1/Tau5, pT181/Tau5, pT231/Tau5 and p-ERK/ERK were all higher in homozygous hybrid mice (PSEN1G378E/G378E; Tyrobp-/- mice) compared with PSEN1G378E/G378E mice (all P < 0.05). Conclusions TYROBP deficiency might play a protective role in the modulation of neuroinflammation of AD. However, the relationship between neuroinflammation processes involving microglia and astrocyte activation, and release of pro-inflammatory cytokines, and p-Tau pathology needs further study.
Mice
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Animals
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Alzheimer Disease/genetics*
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Neuroinflammatory Diseases
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Hippocampus/pathology*
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Mutation
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Cytokines/pharmacology*
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Disease Models, Animal
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tau Proteins/pharmacology*
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Amyloid beta-Peptides/metabolism*
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Adaptor Proteins, Signal Transducing/pharmacology*
10.Estrogen receptor alpha and colocalization of Bcl-2 in hippocampus of Alzheimer's disease.
Mei ZENG ; Ya-ping LU ; Hao XU ; Jiang-ning ZHOU
Acta Academiae Medicinae Sinicae 2004;26(2):108-111
OBJECTIVETo investigate the possible mechanism by which estrogen regulates apoptosis through the estrogen receptor.
METHODSBy means of fluorescence immunocytochemistry, the present study investigated the distribution of Bcl-2 and the colocolization of Bcl-2 and ERalpha immunoreactivity in the hippocampus of 10 Alzheimer's disease (AD) patients and 10 aged controls.
RESULTSBcl-2 immunoreactivity was widely distributed in neurons, concentrating predominantly on the subfields CA3 and CA4 in the stratum pyramidale of hippocampus both in controls and in AD patients. Bcl-2 staining in the labeled neuron was observed mainly in the cytoplasm and neuritic processes, but a few nuclei were also positive. Bcl-2 labeling was also detected in the astrocytes mainly in AD, but sparsely in controls. Double-labeled fluorescence immunocytochemistry showed that most Bcl-2-immunolabeled neurons also exhibited positive staining for ERalpha.
CONCLUSIONSEstrogen may function as a regulator of apoptosis to modulate the expression of Bcl-2 in neurons and astrocytes in hippocampus of AD through ERalpha.
Alzheimer Disease ; genetics ; metabolism ; Apoptosis ; Astrocytes ; metabolism ; Estrogen Receptor alpha ; Female ; Hippocampus ; metabolism ; pathology ; Humans ; Neurons ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; biosynthesis ; genetics ; Receptors, Estrogen ; genetics ; metabolism ; physiology