The propose of this study was to evaluate the difference of cellular activity dependent on intermittent compressive force by determining the alkaline phosphatase activity. Alkaline phosphatase activity was measured on control and experimental groups every 24, 48, 72hours. Experimental groups consisted of continous and intermittent compressive group which were compressed by 300g/cm2 of diaphram pump. The intermittent compressive group was connected by timer which was worked on 10 minutes an off 10 minutes. The results were as follows; 1. The alkaline phosphatase activity between control and experimental groups showed not significant difference at compressed 24 hours. 2. The alkaline phosphatase activity of experimental groups were more increased than control group at compressed 48 hours. 3. The alkaline phosphatase activity of intermittent compressive group showed significant increased to control group. Whereby continuous compressive group showed not significant difference to control at 72 hours. 4. The alkaline phosphatase activity of intermittent compressive group were stringly increased than continuous compressive groups. 5. Between experimental groups and control group no other morphologic changes were detected by microscopic findings.
Alkaline Phosphatase*

The propose of this study was to evaluate the effect of cellular activity of PDL cells dependent on intermittent and continuous compressive force by determining the alkaline phosphatase activity. An intermittent and continuous compressive forces were applied on PDL cells at the confluent stage. The alkaline phosphatase activity was measured on control and experimental groups every 24, 48, 72hours. The experimental group were consist of continous and intermittent compressive group which were compressed by 300g/cm2 of diaphragm pump. The intermittent compressive group was connected by timer which was worked on 10 minutes and off 10minutes. The results were as follow; 1. The alkaline phosphatase activity of intermittent compressive group was lower than control group at 24 hours(P<0.05). 2. The alkaline phosphatase activity between each groups showed no significant difference at 48hours. 3. The alkaline phosphatase activity of continuous compressive group was significantly higher than control group at 72 hours(P<0.01).
Alkaline Phosphatase* ; Diaphragm ; Periodontal Ligament*

No abstract available.
Alkaline Phosphatase* ; Child* ; Humans ; Leukocytes* ; Leukocytosis*

This study was designed to examine the effect of polyP on the periodontal ligament fibroblasts. The cells were incubated for 24 and 48 hours with various concentrations (0.001%, 0.002%, 0.005%, 0.010%, 0.020%, 0.040%) of polyP. Cell activity, alkaline phosphatase activity, and protein synthesis activity of the cells were examined. The results were as follows: 1. Twenty four hours after incubation with polyP, the cell activity of the periodontal ligament fibroblasts was increased. Forty eight hours after polyP application, the activity of the cells was similar to that of control group. 2. Twenty four or forty eight hours after incubation with polyP, the alkaline phosphatase activity of the periodontal ligament fibroblasts was increased. 3. The protein synthesis of the periodontal ligament fibroblasts was not changed after polyP application. These results suggest that, polyP may increase the cell activity and alkaline phosphatase activity of periodotal ligament fibroblasts and polyP may not affect the protein synthesis of the cells.
Alkaline Phosphatase ; Fibroblasts ; Ligaments ; Periodontal Ligament* ; Polyps

No abstract available.
Alkaline Phosphatase* ; Calcium* ; Female ; Humans ; Osteocalcin*

Methods, data, and discussions of experiments concerning agglutination of human leukemic leukocytes with guinea pig serum and the alkaline phosphatase staining technique by the Azo-dye method are given. An important fact evident from studies of alkaline phosphatase is that there can be chemical differences between cells whose morphology is identical. Even though the results obtained here in the small group of leukemic cases studied by the guinea pig serum agglutination method are not very reproducible, the idea that an antigen-antibody reaction for the determination of leukemia exists, very appealing.
Agglutination ; *Alkaline Phosphatase ; Leukemia/*diagnosis ; *Leukocytes

80 adult rats were divided into 2 groups of 60 rats before and 20 after kidney transplantion. Study showed that the changes of alkaline phosphatase in kidney before transplation trended to vary paralelly with warm ischemic time (WIT) and cold ischemic time (CIT) and the 5 times higher rate of perfusion than normal did not make the enzyme changed. Post transplantion kidney tissue alkaline phosphatase level varied in correlation with the active capacity of transplanted kidney. Blood creatinine level of the rats within a week after the transplantation has a negative variation with enzyme level
Kidney ; Rats ; Kidney Transplantation ; Alkaline Phosphatase ; Enzymes

No abstract available.
Alkaline Phosphatase ; Cell Adhesion ; Fibronectins* ; Humans* ; Osteoblasts

To evaluate the effect of a static magnetic field on the bone producing potential of MC3T3 El cells, the alkaline phosphatase activity was measured after the cells having been cultured under 76.4mT static magnetic field using a SmCos magnets for 5days, 7days, lldays, 15days and 2ldays for each cell culture group. Also, the amount of bone nodule stained with Alizarin red S was observed. The results were as follows. · The alkaline phosphatase activity of the 7, 11, and 15 days group among the experimental groups was decreased as compared with the control groups, and the decrease of alkaline phosphatase activity in the 11 days group was the most evident among them. · Any stained bone nodules of both groups had not been observed until the 11th day. The stained bone nodules in the control groups were found on the 15th day, but not in the experimental groups. The stained bone nodules were observed in both groups on the 21st day, but the control groups have more bone nodules than the experimental groups.
Alkaline Phosphatase ; Cell Culture Techniques ; Magnetic Fields*

ABSTRACT The goal of this study was to investigate differences in alkaline phosphatase (ALP) levels in young and old rabbits after administering the soybean isoflavone genistein during orthodontic tooth movement. Twelve rabbits were used and assigned to four groups (n = 3); OG (old rabbits), OGS (old rabbits + soybean), YG (young rabbits), and YGS (young rabbits + soybean). The rabbit mandibulary incisors were distalised using a nickel-titanium open coil spring (50 g force). Genistein was administered from the initial orthodontic force delivery until day 21, at a dose of 1.2 mg/kg BW once a day. ALP levels (U/mg) were measured on days 1, 7, 14, 21 after orthodontic force delivery using a UV-Vis 6300 spectrophotometer at a 405 nm wavelength. The results were analysed by one-way analysis of variance followed by Tukey’s Honest Significant Difference (HSD) test (p < 0.05). The ALP levels between the young and old age groups were significantly different. ALP levels were highest in the YGS group, and significantly lowest in the OG group (p < 0.05). Moreover, the ALP level of the OGS group was significantly higher than that in the OG group (p < 0.05). In conclusion, daily consumption of soybean isoflavone genistein could enhance ALP levels during orthodontic tooth movement, particularly in older rabbits.
Alkaline Phosphatase ; Tooth Movement Techniques ; Rabbits